(7)
(8)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7601, RRID:AB_2841092.
展开/折叠
AI608257; bHLHd2; Class D basic helix-loop-helix protein 2; OTTHUMP00000028740; Processed sterol regulatory element-binding protein 2; SRBP2_HUMAN; SREBF 2; Srebf2; SREBF2 protein; SREBP 2; SREBP-2; SREBP2; SREBP2gc; sterol regulatory element binding factor 2; Sterol regulatory element binding protein 2; Sterol regulatory element binding transcription factor 2; Sterol regulatory element-binding transcription factor 2;
抗原和靶标
- Q12772 SRBP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDSGELGGLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGSGGSGSSSGSSGSSSSSSNGRGSSSGAVDPSVQRSFTQVTLPSFSPSAASPQAPTLQVKVSPTSVPTTPRATPILQPRPQPQPQPQTQLQQQTVMITPTFSTTPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSSGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNEVDLKIEDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTSLLQWGGAHDSDQHPHSGSGRSVLSFESGSGGWFDWMMPTLLLWLVNGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAGNLQTCLAVLGRALPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQCRRATPATEAGFEDEAKTSARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTRCGGKLGFLASYFLSRAQSLCGPEHSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAIESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSVGVMSPPLSRSSVLKSALGPDIICRWWTSAITVAISWLQGDDAAVRSHFTKVERIPKALEVTESPLVKAIFHACRAMHASLPGKADGQQSSFCHCERASGHLWSSLNVSGATSDPALNHVVQLLTCDLLLSLRTALWQKQASASQAVGETYHASGAELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTTQSTKHGEVDAWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRRSCNDCQQMIVKLGGGTAIAAS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q12772 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S79 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| T155 | Phosphorylation | Uniprot | |
| Y171 | Phosphorylation | Uniprot | |
| K249 | Sumoylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K321 | Acetylation | Uniprot | |
| K340 | Ubiquitination | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| K363 | Acetylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| K420 | Sumoylation | Uniprot | |
| S432 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S455 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| K464 | Sumoylation | Uniprot | |
| K464 | Ubiquitination | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| K630 | Acetylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| K660 | Ubiquitination | Uniprot | |
| K731 | Ubiquitination | Uniprot | |
| S745 | Phosphorylation | Uniprot | |
| K768 | Ubiquitination | Uniprot | |
| K778 | Ubiquitination | Uniprot | |
| K782 | Ubiquitination | Uniprot | |
| K805 | Ubiquitination | Uniprot | |
| K817 | Ubiquitination | Uniprot | |
| K821 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| Y840 | Phosphorylation | Uniprot | |
| S855 | Phosphorylation | Uniprot | |
| K865 | Ubiquitination | Uniprot | |
| K901 | Ubiquitination | Uniprot | |
| K907 | Ubiquitination | Uniprot | |
| K918 | Ubiquitination | Uniprot | |
| K934 | Ubiquitination | Uniprot | |
| K989 | Ubiquitination | Uniprot | |
| K1031 | Ubiquitination | Uniprot | |
| S1046 | Phosphorylation | Uniprot | |
| S1057 | Phosphorylation | Uniprot | |
| K1067 | Ubiquitination | Uniprot | |
| S1094 | Phosphorylation | Uniprot | |
| S1098 | Phosphorylation | Uniprot | |
| K1115 | Ubiquitination | Uniprot | |
| S1121 | Phosphorylation | Uniprot |
研究背景
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.
Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.
Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Nucleus.
Ubiquitously expressed in adult and fetal tissues.
Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts (via C-terminal domain) with RNF139.
Belongs to the SREBP family.
文献引用
Application: WB Species: rat Sample: BRL-3A hepatocytes
Application: WB Species: Human Sample: VSMC
Application: WB Species: human Sample: MG63 cells
Application: IHC Species: human Sample: Migrated Saos-2 and MG63 cells
Application: WB Species: Rat Sample:
Application: WB Species: Rat Sample: liver
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