产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0682, RRID:AB_2834390.
展开/折叠
EHB21; EPN 2; Epn2; EPN2_HUMAN; EPS 15 interacting protein 2; EPS-15-interacting protein 2; Eps15 binding protein; Epsin 2; Epsin-2; Epsin2; KIAA1065; OTTHUMP00000065808; OTTHUMP00000065809; OTTHUMP00000065810; OTTHUMP00000065811; OTTHUMP00000065889;
抗原和靶标
- O95208 EPN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKNWRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERLKAERAQALKTKERMAQVATGMGSNQITFGRGSSQPNLSTSHSEQEYGKAGGSPASYHGSPEASLCPQHRTGAPLGQSEELQPLSQRHPFLPHLGLASRPNGDWSQPCLTCDRAARATSPRVSSELEQARPQTSGEEELQLQLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKIPKKKEHGSLPQQTTLLDLMDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATVQSVPKNSDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSLPSQNNGTTSPDPFESQPLTVASSKPSSARKTPESFLGPNAALVNLDSLVTRPAPPAQSLNPFLAPGAPATSAPVNPFQVNQPQPLTLNQLRGSPVLGTSTSFGPGPGVESMAVASMTSAAPQPALGATGSSLTPLGPAMMNMVGSVGIPPSAAQATGTTNPFLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95208 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Phosphorylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| R170 | Methylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| Y186 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| T272 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| T316 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| K423 | Acetylation | Uniprot | |
| K423 | Methylation | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| S426 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| S492 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| T508 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S622 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot |
研究背景
Plays a role in the formation of clathrin-coated invaginations and endocytosis.
Ubiquitinated.
Cytoplasm. Cytoplasmic vesicle>Clathrin-coated vesicle.
Note: In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex.
Highest expression is found in brain. Detected at lower levels in lung and liver.
Binds EPS15 (By similarity). Interacts with ITSN1 (By similarity). Binds AP-2 and clathrin. Interacts with UBQLN2.
The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding.
Belongs to the epsin family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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