(1)
(7)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7607, RRID:AB_2841098.
展开/折叠
cell proliferation-inducing protein 59; GLNA; GLNA_HUMAN; GLNS; GLUL; Glutamate ammonia ligase; Glutamate decarboxylase; Glutamate--ammonia ligase; glutamine synthase; Glutamine synthetase; GS; PIG 43; PIG 59; PIG43; PIG59; Proliferation inducing protein 43;
抗原和靶标
- P15104 GLNA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15104 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| K11 | Acetylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| K14 | Acetylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K103 | Acetylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| C183 | S-Nitrosylation | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| C359 | S-Nitrosylation | Uniprot | |
| Y371 | Phosphorylation | Uniprot | |
| K372 | Acetylation | Uniprot | |
| K372 | Ubiquitination | Uniprot |
研究背景
Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis.
Palmitoylated; undergoes autopalmitoylation.
Ubiquitinated by ZNRF1.
Cytoplasm>Cytosol. Microsome. Mitochondrion. Cell membrane>Lipid-anchor.
Note: Mainly localizes in the cytosol, with a fraction associated with the cell membrane.
Expressed in endothelial cells.
Decamer; composed of two pentamers. Interacts with PALMD (By similarity). Interacts with RHOJ.
Belongs to the glutamine synthetase family.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Metabolism > Amino acid metabolism > Arginine biosynthesis.
· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.
· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.
· Metabolism > Energy metabolism > Nitrogen metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Nervous system > GABAergic synapse.
文献引用
Application: IF/ICC Species: Human Sample: CRC tissues
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