(1)
(3)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0180, RRID:AB_2833373.
展开/折叠
DKFZP586J0917; FLJ99588; HD 7a; HD7; HD7a; HDAC 7; HDAC 7A; Hdac7; HDAC7_HUMAN; HDAC7A; Histone deacetylase 7; Histone deacetylase 7A; OTTHUMP00000202813; OTTHUMP00000202814;
抗原和靶标
- Q8WUI4 HDAC7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDLRVGQRPPVEPPPEPTLLALQRPQRLHHHLFLAGLQQQRSVEPMRLSMDTPMPELQVGPQEQELRQLLHKDKSKRSAVASSVVKQKLAEVILKKQQAALERTVHPNSPGIPYRTLEPLETEGATRSMLSSFLPPVPSLPSDPPEHFPLRKTVSEPNLKLRYKPKKSLERRKNPLLRKESAPPSLRRRPAETLGDSSPSSSSTPASGCSSPNDSEHGPNPILGSEALLGQRLRLQETSVAPFALPTVSLLPAITLGLPAPARADSDRRTHPTLGPRGPILGSPHTPLFLPHGLEPEAGGTLPSRLQPILLLDPSGSHAPLLTVPGLGPLPFHFAQSLMTTERLSGSGLHWPLSRTRSEPLPPSATAPPPPGPMQPRLEQLKTHVQVIKRSAKPSEKPRLRQIPSAEDLETDGGGPGQVVDDGLEHRELGHGQPEARGPAPLQQHPQVLLWEQQRLAGRLPRGSTGDTVLLPLAQGGHRPLSRAQSSPAAPASLSAPEPASQARVLSSSETPARTLPFTTGLIYDSVMLKHQCSCGDNSRHPEHAGRIQSIWSRLQERGLRSQCECLRGRKASLEELQSVHSERHVLLYGTNPLSRLKLDNGKLAGLLAQRMFVMLPCGGVGVDTDTIWNELHSSNAARWAAGSVTDLAFKVASRELKNGFAVVRPPGHHADHSTAMGFCFFNSVAIACRQLQQQSKASKILIVDWDVHHGNGTQQTFYQDPSVLYISLHRHDDGNFFPGSGAVDEVGAGSGEGFNVNVAWAGGLDPPMGDPEYLAAFRIVVMPIAREFSPDLVLVSAGFDAAEGHPAPLGGYHVSAKCFGYMTQQLMNLAGGAVVLALEGGHDLTAICDASEACVAALLGNRVDPLSEEGWKQKPNLNAIRSLEAVIRVHSKYWGCMQRLASCPDSWVPRVPGADKEEVEAVTALASLSVGILAEDRPSEQLVEEEEPMNL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8WUI4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S42 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| T104 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| Y114 | Phosphorylation | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Q7KZI7 (MARK2) , Q15139 (PRKD1) , P27448 (MARK3) , Q13131 (PRKAA1) , O43318 (MAP3K7) , P98161 (PKD1) | Uniprot |
| K160 | Sumoylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| T247 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| R277 | Methylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Q13131 (PRKAA1) , Q15139 (PRKD1) | Uniprot |
| S364 | Phosphorylation | Uniprot | |
| T366 | Phosphorylation | Uniprot | |
| K382 | Sumoylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S482 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| S487 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| S508 | Phosphorylation | Uniprot | |
| T511 | Phosphorylation | Uniprot | |
| T515 | Phosphorylation | Uniprot | |
| Y524 | Phosphorylation | Uniprot | |
| S526 | Phosphorylation | Uniprot | |
| K530 | Methylation | Uniprot | |
| K530 | Ubiquitination | Uniprot | |
| S534 | Phosphorylation | Uniprot | |
| K571 | Ubiquitination | Uniprot | |
| S573 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| K603 | Acetylation | Uniprot | |
| K603 | Ubiquitination | Uniprot | |
| K893 | Ubiquitination | Uniprot | |
| S903 | Phosphorylation | Uniprot | |
| S907 | Phosphorylation | Uniprot |
研究背景
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. Positively regulates the transcriptional repressor activity of FOXP3. Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.
May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylation at Ser-155 by MARK2, MARK3 and PRKD1 promotes interaction with 14-3-3 proteins and export from the nucleus. Phosphorylation at Ser-155 is a prerequisite for phosphorylation at Ser-181.
Nucleus. Cytoplasm.
Note: In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and is due to its phosphorylation.
Interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3. Interacts with the 14-3-3 protein YWHAE, MEF2A, MEF2B and MEF2C (By similarity). Interacts with KAT5 and EDNRA. Interacts with KDM5B. Interacts with ZMYND15 (By similarity). Interacts with PML (isoform PML-4). Interacts with FOXP3. Interacts with RARA.
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Belongs to the histone deacetylase family. HD type 2 subfamily.
研究领域
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
文献引用
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