| 产品: | Huntingtin 抗体 |
| 货号: | DF7663 |
| 描述: | Rabbit polyclonal antibody to Huntingtin |
| 应用: | WB |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep, Chicken |
| 分子量: | 348kDa; 348kD(Calculated). |
| 蛋白号: | P42858 |
| RRID: | AB_2841137 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7663, RRID:AB_2841137.
展开/折叠
AI256365; C430023I11Rik; HD; HD protein; HD_HUMAN; HDH; HTT; Huntingtin; HUNTINGTON CHOREA; Huntington disease protein; Huntington's disease protein homolog; IT 15; IT15; OTTMUSP00000026909; ZHD;
抗原和靶标
Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
- P42858 HD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPKKELSATKKDRVNHCLTICENIVAQSVRNSPEFQKLLGIAMELFLLCSDDAESDVRMVADECLNKVIKALMDSNLPRLQLELYKEIKKNGAPRSLRAALWRFAELAHLVRPQKCRPYLVNLLPCLTRTSKRPEESVQETLAAAVPKIMASFGNFANDNEIKVLLKAFIANLKSSSPTIRRTAAGSAVSICQHSRRTQYFYSWLLNVLLGLLVPVEDEHSTLLILGVLLTLRYLVPLLQQQVKDTSLKGSFGVTRKEMEVSPSAEQLVQVYELTLHHTQHQDHNVVTGALELLQQLFRTPPPELLQTLTAVGGIGQLTAAKEESGGRSRSGSIVELIAGGGSSCSPVLSRKQKGKVLLGEEEALEDDSESRSDVSSSALTASVKDEISGELAASSGVSTPGSAGHDIITEQPRSQHTLQADSVDLASCDLTSSATDGDEEDILSHSSSQVSAVPSDPAMDLNDGTQASSPISDSSQTTTEGPDSAVTPSDSSEIVLDGTDNQYLGLQIGQPQDEDEEATGILPDEASEAFRNSSMALQQAHLLKNMSHCRQPSDSSVDKFVLRDEATEPGDQENKPCRIKGDIGQSTDDDSAPLVHCVRLLSASFLLTGGKNVLVPDRDVRVSVKALALSCVGAAVALHPESFFSKLYKVPLDTTEYPEEQYVSDILNYIDHGDPQVRGATAILCGTLICSILSRSRFHVGDWMGTIRTLTGNTFSLADCIPLLRKTLKDESSVTCKLACTAVRNCVMSLCSSSYSELGLQLIIDVLTLRNSSYWLVRTELLETLAEIDFRLVSFLEAKAENLHRGAHHYTGLLKLQERVLNNVVIHLLGDEDPRVRHVAAASLIRLVPKLFYKCDQGQADPVVAVARDQSSVYLKLLMHETQPPSHFSVSTITRIYRGYNLLPSITDVTMENNLSRVIAAVSHELITSTTRALTFGCCEALCLLSTAFPVCIWSLGWHCGVPPLSASDESRKSCTVGMATMILTLLSSAWFPLDLSAHQDALILAGNLLAASAPKSLRSSWASEEEANPAATKQEEVWPALGDRALVPMVEQLFSHLLKVINICAHVLDDVAPGPAIKAALPSLTNPPSLSPIRRKGKEKEPGEQASVPLSPKKGSEASAASRQSDTSGPVTTSKSSSLGSFYHLPSYLKLHDVLKATHANYKVTLDLQNSTEKFGGFLRSALDVLSQILELATLQDIGKCVEEILGYLKSCFSREPMMATVCVQQLLKTLFGTNLASQFDGLSSNPSKSQGRAQRLGSSSVRPGLYHYCFMAPYTHFTQALADASLRNMVQAEQENDTSGWFDVLQKVSTQLKTNLTSVTKNRADKNAIHNHIRLFEPLVIKALKQYTTTTCVQLQKQVLDLLAQLVQLRVNYCLLDSDQVFIGFVLKQFEYIEVGQFRESEAIIPNIFFFLVLLSYERYHSKQIIGIPKIIQLCDGIMASGRKAVTHAIPALQPIVHDLFVLRGTNKADAGKELETQKEVVVSMLLRLIQYHQVLEMFILVLQQCHKENEDKWKRLSRQIADIILPMLAKQQMHIDSHEALGVLNTLFEILAPSSLRPVDMLLRSMFVTPNTMASVSTVQLWISGILAILRVLISQSTEDIVLSRIQELSFSPYLISCTVINRLRDGDSTSTLEEHSEGKQIKNLPEETFSRFLLQLVGILLEDIVTKQLKVEMSEQQHTFYCQELGTLLMCLIHIFKSGMFRRITAAATRLFRSDGCGGSFYTLDSLNLRARSMITTHPALVLLWCQILLLVNHTDYRWWAEVQQTPKRHSLSSTKLLSPQMSGEEEDSDLAAKLGMCNREIVRRGALILFCDYVCQNLHDSEHLTWLIVNHIQDLISLSHEPPVQDFISAVHRNSAASGLFIQAIQSRCENLSTPTMLKKTLQCLEGIHLSQSGAVLTLYVDRLLCTPFRVLARMVDILACRRVEMLLAANLQSSMAQLPMEELNRIQEYLQSSGLAQRHQRLYSLLDRFRLSTMQDSLSPSPPVSSHPLDGDGHVSLETVSPDKDWYVHLVKSQCWTRSDSALLEGAELVNRIPAEDMNAFMMNSEFNLSLLAPCLSLGMSEISGGQKSALFEAAREVTLARVSGTVQQLPAVHHVFQPELPAEPAAYWSKLNDLFGDAALYQSLPTLARALAQYLVVVSKLPSHLHLPPEKEKDIVKFVVATLEALSWHLIHEQIPLSLDLQAGLDCCCLALQLPGLWSVVSSTEFVTHACSLIYCVHFILEAVAVQPGEQLLSPERRTNTPKAISEEEEEVDPNTQNPKYITAACEMVAEMVESLQSVLALGHKRNSGVPAFLTPLLRNIIISLARLPLVNSYTRVPPLVWKLGWSPKPGGDFGTAFPEIPVEFLQEKEVFKEFIYRINTLGWTSRTQFEETWATLLGVLVTQPLVMEQEESPPEEDTERTQINVLAVQAITSLVLSAMTVPVAGNPAVSCLEQQPRNKPLKALDTRFGRKLSIIRGIVEQEIQAMVSKRENIATHHLYQAWDPVPSLSPATTGALISHEKLLLQINPERELGSMSYKLGQVSIHSVWLGNSITPLREEEWDEEEEEEADAPAPSSPPTSPVNSRKHRAGVDIHSCSQFLLELYSRWILPSSSARRTPAILISEVVRSLLVVSDLFTERNQFELMYVTLTELRRVHPSEDEILAQYLVPATCKAAAVLGMDKAVAEPVSRLLESTLRSSHLPSRVGALHGVLYVLECDLLDDTAKQLIPVISDYLLSNLKGIAHCVNIHSQQHVLVMCATAFYLIENYPLDVGPEFSASIIQMCGVMLSGSEESTPSIIYHCALRGLERLLLSEQLSRLDAESLVKLSVDRVNVHSPHRAMAALGLMLTCMYTGKEKVSPGRTSDPNPAAPDSESVIVAMERVSVLFDRIRKGFPCEARVVARILPQFLDDFFPPQDIMNKVIGEFLSNQQPYPQFMATVVYKVFQTLHSTGQSSMVRDWVMLSLSNFTQRAPVAMATWSLSCFFVSASTSPWVAAILPHVISRMGKLEQVDVNLFCLVATDFYRHQIEEELDRRAFQSVLEVVAAPGSPYHRLLTCLRNVHKVTTC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P42858 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T3 | Phosphorylation | Uniprot | |
| K6 | Acetylation | Uniprot | |
| K6 | Sumoylation | Uniprot | |
| K9 | Acetylation | Uniprot | |
| K9 | Sumoylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| K15 | Acetylation | Uniprot | |
| K15 | Sumoylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K96 | Acetylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K176 | Acetylation | Uniprot | |
| K234 | Acetylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| T269 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| C278 | S-Nitrosylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| K343 | Acetylation | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | O00141 (SGK1) , P31749 (AKT1) | Uniprot |
| S429 | Phosphorylation | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S436 | Phosphorylation | Uniprot | |
| K442 | Acetylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| T486 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| S534 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| S640 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S643 | Phosphorylation | Uniprot | |
| T654 | Phosphorylation | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| K667 | Ubiquitination | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| T796 | Phosphorylation | Uniprot | |
| K813 | Ubiquitination | Uniprot | |
| K902 | Ubiquitination | Uniprot | |
| S930 | Phosphorylation | Uniprot | |
| K937 | Ubiquitination | Uniprot | |
| K941 | Ubiquitination | Uniprot | |
| S1104 | Phosphorylation | Uniprot | |
| S1111 | Phosphorylation | Uniprot | |
| T1120 | Phosphorylation | Uniprot | |
| K1121 | Ubiquitination | Uniprot | |
| T1173 | Phosphorylation | Uniprot | |
| S1177 | Phosphorylation | Uniprot | |
| S1179 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S1195 | Phosphorylation | Uniprot | |
| S1199 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S1210 | Phosphorylation | Uniprot | |
| T1215 | Phosphorylation | Uniprot | |
| K1223 | Ubiquitination | Uniprot | |
| S1229 | Phosphorylation | Uniprot | |
| K1244 | Ubiquitination | Uniprot | |
| K1262 | Ubiquitination | Uniprot | |
| T1282 | Phosphorylation | Uniprot | |
| T1322 | Phosphorylation | Uniprot | |
| K1337 | Ubiquitination | Uniprot | |
| S1348 | Phosphorylation | Uniprot | |
| S1349 | Phosphorylation | Uniprot | |
| Y1355 | Phosphorylation | Uniprot | |
| K1402 | Ubiquitination | Uniprot | |
| K1410 | Ubiquitination | Uniprot | |
| K1415 | Ubiquitination | Uniprot | |
| K1431 | Ubiquitination | Uniprot | |
| S1805 | Phosphorylation | Uniprot | |
| S1811 | Phosphorylation | Uniprot | |
| Y1813 | Phosphorylation | Uniprot | |
| T1814 | Phosphorylation | Uniprot | |
| S1862 | Phosphorylation | Uniprot | |
| S1864 | Phosphorylation | Uniprot | |
| T1866 | Phosphorylation | Uniprot | |
| S1870 | Phosphorylation | Uniprot | |
| S1874 | Phosphorylation | Uniprot | |
| S1880 | Phosphorylation | Uniprot | |
| K1885 | Ubiquitination | Uniprot | |
| S2074 | Phosphorylation | Uniprot | |
| S2233 | Phosphorylation | Uniprot | |
| T2335 | Phosphorylation | Uniprot | |
| K2337 | Acetylation | Uniprot | |
| S2340 | Phosphorylation | Uniprot | |
| S2382 | Phosphorylation | Uniprot | |
| K2417 | Ubiquitination | Uniprot | |
| K2423 | Ubiquitination | Uniprot | |
| K2443 | Ubiquitination | Uniprot | |
| K2537 | Ubiquitination | Uniprot | |
| S2548 | Phosphorylation | Uniprot | |
| K2564 | Ubiquitination | Uniprot | |
| S2651 | Phosphorylation | Uniprot | |
| T2654 | Phosphorylation | Uniprot | |
| S2655 | Phosphorylation | Uniprot | |
| S2708 | Phosphorylation | Uniprot | |
| T2712 | Phosphorylation | Uniprot | |
| K2757 | Ubiquitination | Uniprot | |
| S2764 | Phosphorylation | Uniprot | |
| S2892 | Phosphorylation | Uniprot | |
| K2901 | Ubiquitination | Uniprot | |
| S2934 | Phosphorylation | Uniprot | |
| T2938 | Phosphorylation | Uniprot | |
| S2948 | Phosphorylation | Uniprot | |
| S2950 | Phosphorylation | Uniprot | |
| K2967 | Ubiquitination | Uniprot |
研究背景
May play a role in microtubule-mediated transport or vesicle function.
Promotes the formation of autophagic vesicles.
Cleaved by caspases downstream of the polyglutamine stretch. The resulting N-terminal fragments are cytotoxic and provokes apoptosis.
Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
Phosphorylation at Ser-1179 and Ser-1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity.
Myristoylated at Gly-551, following proteolytic cleavage at Asp-550.
Cytoplasm. Nucleus. Early endosome.
Note: The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington disease neurons. Shuttles between cytoplasm and nucleus in a Ran GTPase-independent manner (PubMed:15654337). Recruits onto early endosomes in a Rab5- and HAP40-dependent fashion (PubMed:16476778).
Cytoplasmic vesicle>Autophagosome.
Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
Interacts with PFN1. Interacts through its N-terminus with PRPF40A. Interacts with PQBP1. Interacts with SETD2. Interacts with SH3GLB1 (By similarity). Interacts with SYVN. Interacts with TPR; the interaction is inhibited by forms of Huntingtin with expanded polyglutamine stretch. Interacts with ZDHHC13 (via ANK repeats). Interacts with ZDHHC17 (via ANK repeats). Interacts with F8A1/F8A2/F8A3. Found in a complex with F8A1/F8A2/F8A3, HTT and RAB5A; mediates the recruitment of HTT by RAB5A.
The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
Belongs to the huntingtin family.
研究领域
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
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