(4)
(4)
| 产品: | FADD 抗体 |
| 货号: | DF7674 |
| 描述: | Rabbit polyclonal antibody to FADD |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Bovine, Sheep |
| 分子量: | 28 kDa; 23kD(Calculated). |
| 蛋白号: | Q13158 |
| RRID: | AB_2841146 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7674, RRID:AB_2841146.
展开/折叠
FADD; FADD protein; FADD_HUMAN; Fas (TNFRSF6) associated via death domain; Fas associated via death domain; Fas associating death domain containing protein; Fas associating protein; Fas associating protein with death domain; Fas TNFRSF6 associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; GIG 3; GIG3; Growth inhibiting gene 3 protein; Growth-inhibiting gene 3 protein; H sapiens mRNA for mediator of receptor induced toxicity; Mediator of receptor induced toxicity; MGC8528; MORT 1; MORT1; Protein FADD;
抗原和靶标
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
- Q13158 FADD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDLFSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSDTKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVGALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSWNSDASTSEAS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13158 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| K120 | Sumoylation | Uniprot | |
| K125 | Sumoylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| R132 | Methylation | Uniprot | |
| Y133 | Phosphorylation | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| K149 | Sumoylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| T157 | Phosphorylation | Uniprot | |
| R166 | Methylation | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | P53350 (PLK1) , O14733 (MAP2K7) , Q9BV47 (DUSP26) , Q9H422 (HIPK3) , P48729 (CSNK1A1) , Q13233 (MAP3K1) | Uniprot |
| S197 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | P67870 (CSNK2B) | Uniprot |
| S203 | Phosphorylation | O14965 (AURKA) | Uniprot |
研究背景
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Can self-associate. Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and PIDD1. Interacts (via death domain) with FAS (via death domain). Interacts with CASP8. Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation (By similarity). Interacts with RIPK1, TRADD and CASP8 (By similarity). Interacts with stimulated TNFRSF10B.
(Microbial infection) Interacts with human papillomavirus 16/HPV16 protein E6.
(Microbial infection) Interacts with molluscum contagiosum virus proteins MC159L/v-CFLAR and MC160L.
Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
The interaction between the FAS and FADD death domains is crucial for the formation of the death-inducing signaling complex (DISC).
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
文献引用
Application: WB Species: human Sample: Caco2 cells
Application: IHC Species: mouse Sample: colon
Application: WB Species: rat Sample: intestinal
Application: WB Species: bovine Sample: bEECs
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.