| 产品: | IHH 抗体 |
| 货号: | DF7688 |
| 描述: | Rabbit polyclonal antibody to IHH |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Zebrafish, Sheep, Rabbit, Chicken, Xenopus |
| 分子量: | 45 kDa; 45kD(Calculated). |
| 蛋白号: | Q14623 |
| RRID: | AB_2841159 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7688, RRID:AB_2841159.
展开/折叠
BDA1; HHG-2; HHG2; IHH; IHH_HUMAN; Indian Hedgehog; Indian hedgehog homolog; Indian hedgehog protein; Indian hedgehog protein C-product; Indian hedgehog protein N-product;
抗原和靶标
- Q14623 IHH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSPARLRPRLHFCLVLLLLLVVPAAWGCGPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIARSSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFDWVYYESKAHVHCSVKSEHSAAAKTGGCFPAGAQVRLESGARVALSAVRPGDRVLAMGEDGSPTFSDVLIFLDREPHRLRAFQVIETQDPPRRLALTPAHLLFTADNHTEPAARFRATFASHVQPGQYVLVAGVPGLQPARVAAVSTHVALGAYAPLTKHGTLVVEDVVASCFAAVADHHLAQLAFWPLRLFHSLAWGSWTPGEGVHWYPQLLYRLGRLLLEEGSFHPLGMSGAGS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14623 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N282 | N-Glycosylation | Uniprot | |
| T293 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| Y303 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot |
研究背景
Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) (By similarity).
The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).
Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
Palmitoylated. N-palmitoylation is required for N-product multimerization and full activity.
Cell membrane>Lipid-anchor>Extracellular side.
Note: The N-terminal peptide remains associated with the cell surface.
Secreted>Extracellular space.
Note: The C-terminal peptide diffuses from the cell.
Cell membrane.
Expressed in embryonic lung, and in adult kidney and liver.
Homooligomer (indian hedgehog protein N-product). Interacts with BOC and CDON. Interacts with PTCH1. Interacts with glypican GPC3 (By similarity).
The indian hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain.
Belongs to the hedgehog family.
研究领域
· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
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