ABCG1 Antibody - #DF7693

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产品: ABCG1 抗体
货号: DF7693
描述: Rabbit polyclonal antibody to ABCG1
应用: WB
反应: Human
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
分子量: 110 kDa; 76kD(Calculated).
蛋白号: P45844
RRID: AB_2841164

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
IHC Protocol
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
ABCG1 Antibody detects endogenous levels of total ABCG1.
RRID:
AB_2841164
引用格式: Affinity Biosciences Cat# DF7693, RRID:AB_2841164.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ABC transporter 8; ABC8; ABCG1; ABCG1_HUMAN; ATP-binding cassette sub family G member 1; ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; ATP-binding cassette transporter member 1 of subfamily G; ATP-binding cassette, sub family G WHITE member 1; homolog of Drosophila white; MGC34313; White protein homolog; White protein homolog ATP binding cassette transporter 8; WHITE1; WHT1;

抗原和靶标

免疫原:
Uniprot:

P45844(ABCG1_HUMAN) >>Visit HPA database.

基因/基因ID:
ABCG1(9619)
表达:
P45844 ABCG1_HUMAN:

Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.

序列:
MACLMAAFSVGTAMNASSYSAEMTEPKSVCVSVDEVVSSNMEATETDLLNGHLKKVDNNLTEAQRFSSLPRRAAVNIEFRDLSYSVPEGPWWRKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMKGAVLINGLPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLSCANTRTGSLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKGLAQGGRSIICTIHQPSAKLFELFDQLYVLSQGQCVYRGKVCNLVPYLRDLGLNCPTYHNPADFVMEVASGEYGDQNSRLVRAVREGMCDSDHKRDLGGDAEVNPFLWHRPSEEVKQTKRLKGLRKDSSSMEGCHSFSASCLTQFCILFKRTFLSIMRDSVLTHLRITSHIGIGLLIGLLYLGIGNEAKKVLSNSGFLFFSMLFLMFAALMPTVLTFPLEMGVFLREHLNYWYSLKAYYLAKTMADVPFQIMFPVAYCSIVYWMTSQPSDAVRFVLFAALGTMTSLVAQSLGLLIGAASTSLQVATFVGPVTAIPVLLFSGFFVSFDTIPTYLQWMSYISYVRYGFEGVILSIYGLDREDLHCDIDETCHFQKSEAILRELDVENAKLYLDFIVLGIFFISLRLIAYFVLRYKIRAER

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Zebrafish
83
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P45844 作为底物

Site PTM Type Enzyme
K54 Ubiquitination
K55 Ubiquitination
K98 Ubiquitination
T99 Phosphorylation
K102 Ubiquitination
S105 Phosphorylation
K107 Ubiquitination
K140 Ubiquitination
K189 Ubiquitination
K300 Ubiquitination
K354 Ubiquitination
K376 Ubiquitination

研究背景

功能:

Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrlysis of ATP. The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol.

翻译修饰:

Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.

细胞定位:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cell membrane.
Note: Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.

亚基结构:

Homodimer; disulfide-linked. Homooligomer. May form heterodimers with several heterologous partners of the ABCG subfamily. Interacts with CAV1; this interaction regulates ABCG1-mediated cholesterol efflux.

蛋白家族:

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

研究领域

· Environmental Information Processing > Membrane transport > ABC transporters.

文献引用

1). Guang Chen Pi (the pericarp of Citrus reticulata Blanco's cultivars 'Chachi') inhibits macrophage-derived foam cell formation. JOURNAL OF ETHNOPHARMACOLOGY (PubMed: 35489660) [IF=5.4]
2). Three polymethoxyflavones from the peel of Citrus reticulata “Chachi” inhibits oxidized low-density lipoprotein-induced macrophage-derived foam cell formation. Frontiers in Cardiovascular Medicine (PubMed: 35966555) [IF=3.6]

Application: WB    Species: Mice    Sample: RAW264.7 cells

FIGURE 6 Effects of TAN, HxMF, and HpMF on ox-LDL-induced cholesterol efflux (A); effects of TAN, HxMF, and HpMF on mRNA expression of SRB1 and ABCG1 in ox-LDL-treated RAW264.7 cells (B,C); effects of TAN, HxMF, and HpMF on protein expression of SRB1 in ox-LDL-treated RAW264.7 cells (D–F); and effects of TAN, HxMF, and HpMF on protein expression of ABCG1 in ox-LDL-treated RAW264.7 cells (G–I). All experiments were run in triplicate and data showed mean ± SD. ##p < 0.01 compared with the control group, *p < 0.05 and **p < 0.01 compared with the ox-LDL group.

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