产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7699, RRID:AB_2841170.
展开/折叠
Actin related protein 2/3 complex subunit 5 (16 kD); Actin related protein 2/3 complex subunit 5; Actin related protein 2/3 complex, subunit 5 16kDa; Actin-related protein 2/3 complex subunit 5; ARC16; Arp2/3 complex 16 kDa subunit; Arp2/3 protein complex subunit p16; ARPC 5; Arpc5; ARPC5_HUMAN; dJ127C7.3; MGC88523; p16 Arc; p16-ARC; RP1 127C7.3;
抗原和靶标
- O15511 ARPC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRQGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAMLLQWHEKALAAGGVGSIVRVLTARKTV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15511 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| K3 | Sumoylation | Uniprot | |
| K3 | Ubiquitination | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| Y19 | Phosphorylation | Uniprot | |
| K23 | Sumoylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| R74 | Methylation | Uniprot | |
| S77 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| K87 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| T146 | Phosphorylation | Uniprot |
研究背景
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Polyubiquitinated by RNF128 with 'Lys-63'-linked chains, leading to proteasomal degradation.
Cytoplasm>Cytoskeleton. Cell projection. Nucleus.
Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.
Belongs to the ARPC5 family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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