产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7706, RRID:AB_2841176.
展开/折叠
DCR; DCR1; Dicer 1 ribonuclease type III; Dicer; DICER_HUMAN; DICER1; Double-strand-specific ribonuclease; Endoribonuclease Dicer; Helicase MOI; Helicase with RNase motif; HERNA; KIAA0928; MNG1; RMSE2;
抗原和靶标
- Q9UPY3 DICER_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UPY3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S82 | Phosphorylation | Uniprot | |
| T238 | Phosphorylation | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| Y452 | Phosphorylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| S643 | Phosphorylation | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| Y664 | Phosphorylation | Uniprot | |
| Y668 | Phosphorylation | Uniprot | |
| Y819 | Phosphorylation | Uniprot | |
| T820 | Phosphorylation | Uniprot | |
| S828 | Phosphorylation | Uniprot | |
| S962 | Phosphorylation | Uniprot | |
| T967 | Phosphorylation | Uniprot | |
| T1001 | Phosphorylation | Uniprot | |
| S1016 | Phosphorylation | Uniprot | |
| K1023 | Ubiquitination | Uniprot | |
| Y1091 | Phosphorylation | Uniprot | |
| Y1121 | Phosphorylation | Uniprot | |
| C1122 | S-Nitrosylation | Uniprot | |
| S1142 | Phosphorylation | Uniprot | |
| S1160 | Phosphorylation | Uniprot | |
| Y1204 | Phosphorylation | Uniprot | |
| S1252 | Phosphorylation | Uniprot | |
| S1255 | Phosphorylation | Uniprot | |
| S1280 | Phosphorylation | Uniprot | |
| T1329 | Phosphorylation | Uniprot | |
| Y1330 | Phosphorylation | Uniprot | |
| K1350 | Ubiquitination | Uniprot | |
| K1391 | Acetylation | Uniprot | |
| K1433 | Ubiquitination | Uniprot | |
| Y1438 | Phosphorylation | Uniprot | |
| Y1445 | Phosphorylation | Uniprot | |
| S1460 | Phosphorylation | Uniprot | |
| K1465 | Ubiquitination | Uniprot | |
| K1466 | Ubiquitination | Uniprot | |
| S1468 | Phosphorylation | Uniprot | |
| S1470 | Phosphorylation | Uniprot | |
| Y1479 | Phosphorylation | Uniprot | |
| K1595 | Ubiquitination | Uniprot | |
| S1612 | Phosphorylation | Uniprot | |
| K1615 | Ubiquitination | Uniprot | |
| S1728 | Phosphorylation | Uniprot | |
| S1852 | Phosphorylation | Uniprot | |
| S1868 | Phosphorylation | Uniprot | |
| S1922 | Phosphorylation | Uniprot |
研究背景
Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
Cytoplasm.
Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Associates with the 60S ribosome. Interacts with BCDIN3D. Interacts with AGO2, TARBP2, EIF6, MOV10 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC).
Belongs to the helicase family. Dicer subfamily.
研究领域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
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