UCP1 Antibody - #DF7720

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产品: UCP1 抗体
货号: DF7720
描述: Rabbit polyclonal antibody to UCP1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 34 kDa; 33kD(Calculated).
蛋白号: P25874
RRID: AB_2841189

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%)
克隆:
Polyclonal
特异性:
UCP1 Antibody detects endogenous levels of total UCP1.
RRID:
AB_2841189
引用格式: Affinity Biosciences Cat# DF7720, RRID:AB_2841189.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

mitochondrial brown fat uncoupling protein; Mitochondrial brown fat uncoupling protein 1; SLC25A7; Solute carrier family 25 member 7; Thermogenin; UCP 1; UCP; UCP1; UCP1_HUMAN; uncoupling protein 1 (mitochondrial, proton carrier); Uncoupling protein 1;

抗原和靶标

免疫原:
Uniprot:

P25874(UCP1_HUMAN) >>Visit HPA database.

基因/基因ID:
UCP1(7350)
表达:
P25874 UCP1_HUMAN:

Brown adipose tissue.

序列:
MGGLTASDVHPTLGVQLFSAGIAACLADVITFPLDTAKVRLQVQGECPTSSVIRYKGVLGTITAVVKTEGRMKLYSGLPAGLQRQISSASLRIGLYDTVQEFLTAGKETAPSLGSKILAGLTTGGVAVFIGQPTEVVKVRLQAQSHLHGIKPRYTGTYNAYRIIATTEGLTGLWKGTTPNLMRSVIINCTELVTYDLMKEAFVKNNILADDVPCHLVSALIAGFCATAMSSPVDVVKTRFINSPPGQYKSVPNCAMKVFTNEGPTAFFKGLVPSFLRLGSWNVIMFVCFEQLKRELSKSRQTMDCAT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Xenopus
70
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P25874 作为底物

Site PTM Type Enzyme
S51 Phosphorylation
Y55 Phosphorylation
T61 Phosphorylation
S250 Phosphorylation
T260 Phosphorylation
T265 Phosphorylation

研究背景

功能:

Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates to non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).

翻译修饰:

May undergo sulfenylation upon cold exposure. May increase the sensitivity of UCP1 thermogenic function to the activation by noradrenaline probably through structural effects.

May undergo ubiquitin-mediated proteasomal degradation.

细胞定位:

Mitochondrion inner membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Brown adipose tissue.

亚基结构:

Most probably functions as a monomer. Binds one purine nucleotide per monomer (By similarity). However, has also been suggested to function as a homodimer or a homotetramer. Tightly associates with cardiolipin in the mitochondrion inner membrane; may stabilize and regulate its activity.

蛋白家族:

Belongs to the mitochondrial carrier (TC 2.A.29) family.

研究领域

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

文献引用

1). Atomically precise gold nanoclusters as ROS-scavenging clusterzymes to treat high-fat diet-induced obesity. Chemical Engineering Journal, 2024 [IF=13.3]
2). Pancreastatin mediated regulation of UCP-1 and energy expenditure in high fructose fed perimenopausal rats. Life Sciences, 2021 (PubMed: 34081990) [IF=6.1]

Application: WB    Species: Rat    Sample:

Fig. 4. Potential evidence of UCP-1 enhancement by PST in BAT and WAT of perimenopausal rats. RT-PCR analysis in (A) white and (B) brown adipose representing increased expression of UCP-1. In addition we performed immunoblotting of UCP-1 in (C) WAT and (D) BAT (n = 3). Expression analysis and immunohistochemical staining displayed increased UCP-1 in (E–F) WAT and (G–H) BAT of high fructose fed perimenopausal rats (n = 3). Results are means ± S.E.M. Significance values are #, *p < 0.05; ##, **p < 0.01; ###, ***p < 0.001. Comparative analysis among groups performed as #, Control vs HFrD-VCD and *
3). Co-activating the AMPK signaling axis by low molecular weight fucoidan LF2 and fucoxanthin improves the HFD-induced metabolic syndrome in mice. Journal of Functional Foods, 2022 [IF=5.6]
4). Ndufa8 promotes white fat Browning by improving mitochondrial respiratory chain complex I function to ameliorate obesity by in vitro and in vivo. Cellular signalling, 2024 (PubMed: 39127135) [IF=4.8]
5). Liraglutide promotes UCP1 expression and lipolysis of adipocytes by promoting the secretion of irisin from skeletal muscle cells. Molecular and cellular endocrinology, 2024 (PubMed: 38570133) [IF=4.1]
6). Lactobacillus coryniformis subsp. torquens T3 alleviates non-alcoholic fatty liver disease via reconstruction of the gut microbiota and redox system. Journal of the Science of Food and Agriculture, 2023 (PubMed: 37300818) [IF=4.1]
7). Green tea aqueous extract (GTAE) prevents high-fat diet-induced obesity by activating fat browning. Food Science & Nutrition, 2021 (PubMed: 34925784) [IF=3.9]
8). The mechanisms underlying olanzapine-induced insulin resistance via the brown adipose tissue and the therapy in rats. Adipocyte, 2022 (PubMed: 35067163) [IF=3.3]

Application: WB    Species: Rat    Sample:

Figure 6. The effect of olanzapine and Gegen Qinlian Decoction on the expression of UCP1 in iBAT and scWAT in rat models after 8 weeks gavage. (a) UCP1 expression in scWAT. (b) Representative western blots of UCP1 expression in scWAT. (c) UCP1 expression in iBAT. (d) Representative western blots of UCP1 expression in iBAT. The corresponding control levels are arbitrarily assigned at a value of 1. The values are expressed as mean±SD (n = 6). For statistical significance, *p<0.05 shows a comparison to the BL group; #p<0.05 shows a comparison to the OLZ group.
9). Prdm16-Mediated Browning is Involved in Resistance to Diet-Induced and Monosodium Glutamate-Induced Obesity. Diabetes Metabolic Syndrome and Obesity-Target & Therapy, 2021 (PubMed: 34737591) [IF=3.3]

Application: WB    Species: Mice    Sample: adipose tissue

Figure 5 Prdm16 and Ucp-1 expression in subcutaneous WAT and comparison of serum leptin levels in each group. (A and B) H&E staining in the inguinal subcutaneous WAT of mice induced by DIO and MSG (scale bar = 110 μm). (C and D) Prdm16 expression in the adipose tissue of mice induced by DIO and MSG according to Western blot. (E and F) Ucp-1 expression in the adipose tissue of mice induced by DIO and MSG according to Western blot. (G) Immunoreactivity of Prdm16 in the adipose tissue of mice induced by a high-fat diet and presented as the mean ± SD (n = 3). #P < 0.05 vs the DIO group. (H) Immunoreactivity of Prdm16 in the adipose tissue of mice induced by MSG and presented as the mean ± SD (n = 3). #P < 0.05 vs the MSG group. (I) Immunoreactivity of Ucp-1 in the adipose tissue of mice induced by a high-fat diet and presented as the mean ± SD (n = 3). **P < 0.01 vs the NC group. ##P < 0.01 vs the DIO group. (J) Immunoreactivity of Ucp-1 in the adipose tissue of mice induced by MSG and presented as the mean ± SD (n = 3). **P < 0.01 vs the NC group. ###P < 0.001 vs the MSG group. Abbreviations: DIO, diet-induced obesity; MSG, mice with MSG-induced obesity; NC, normal control; Prdm16, PR domain containing 16; Ucp-1, uncoupling protein-1.
10). Oct-B: A derivative of L-BAIBA significantly alleviating high-fat diet-induced obesity in mice. Biochemical and biophysical research communications, 2024 (PubMed: 39357338) [IF=3.1]

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