产品: Choline Acetyltransferase 抗体
货号: DF7744
描述: Rabbit polyclonal antibody to Choline Acetyltransferase
应用: WB
反应: Human
分子量: 83 kDa.; 83kD(Calculated).
蛋白号: P28329
RRID: AB_2841211

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
Choline Acetyltransferase Antibody detects endogenous levels of total Choline Acetyltransferase.
RRID:
AB_2841211
引用格式: Affinity Biosciences Cat# DF7744, RRID:AB_2841211.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Acetyl CoA choline O acetyltransferase; Acetyl CoA:choline O acetyltransferase; ChAT; CHOACTase; Choline acetylase; choline acetyltransferase; Choline O acetyltransferase; Choline O-acetyltransferase; CLAT_HUMAN; CMS1A; CMS1A2; EC 2.3.1.6; OTTHUMP00000019583; OTTHUMP00000019584;

抗原和靶标

免疫原:

A synthesized peptide derived from human Choline Acetyltransferase, corresponding to a region within C-terminal amino acids.

Uniprot:
基因/基因ID:
序列:
MGLRTAKKRGLGGGGKWKREEGGGTRGRREVRPACFLQSGGRGDPGDVGGPAGNPGCSPHPRAATRPPPLPAHTPAHTPEWCGAASAEAAEPRRAGPHLCIPAPGLTKTPILEKVPRKMAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHVTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

翻译修饰 - P28329 作为底物

Site PTM Type Enzyme
K7 Acetylation
K16 Acetylation
K18 Acetylation
T25 Phosphorylation
T123 Phosphorylation
S125 Phosphorylation
S237 Phosphorylation
S244 Phosphorylation
T373 Phosphorylation P17252 (PRKCA) , P05771 (PRKCB) , P05129 (PRKCG)
S377 Phosphorylation
T462 Phosphorylation
S464 Phosphorylation Q02156 (PRKCE) , P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB)
S465 Phosphorylation P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB) , Q02156 (PRKCE)
S473 Phosphorylation
S475 Phosphorylation
T553 Phosphorylation
Y554 Phosphorylation
S558 Phosphorylation Q05513 (PRKCZ) , Q05655 (PRKCD) , P05771 (PRKCB) , Q02156 (PRKCE) , P17252 (PRKCA) , P05129 (PRKCG)
T574 Phosphorylation Q9UQM7 (CAMK2A) , Q13555 (CAMK2G)
S594 Phosphorylation Q02156 (PRKCE) , P05129 (PRKCG) , Q05513 (PRKCZ) , P17252 (PRKCA) , Q05655 (PRKCD) , P05771 (PRKCB)

研究背景

功能:

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

蛋白家族:

Belongs to the carnitine/choline acetyltransferase family.

研究领域

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Organismal Systems > Nervous system > Cholinergic synapse.

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