产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7755, RRID:AB_2841221.
展开/折叠
[Myelin basic protein]-arginine N-methyltransferase PRMT7; ANM7_HUMAN; FLJ10640; Histone-arginine N-methyltransferase PRMT7; KIAA1933; Myelin basic protein arginine N methyltransferase; OTTHUMP00000174863; PRMT7; Protein arginine methyltransferase 7; Protein arginine N-methyltransferase 7;
抗原和靶标
- Q9NVM4 ANM7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKIFCSRANPTTGSVEWLEEDEHYDYHQEIARSSYADMLHDKDRNVKYYQGIRAAVSRVKDRGQKALVLDIGTGTGLLSMMAVTAGADFCYAIEVFKPMADAAVKIVEKNGFSDKIKVINKHSTEVTVGPEGDMPCRANILVTELFDTELIGEGALPSYEHAHRHLVEENCEAVPHRATVYAQLVESGRMWSWNKLFPIHVQTSLGEQVIVPPVDVESCPGAPSVCDIQLNQVSPADFTVLSDVLPMFSIDFSKQVSSSAACHSRRFEPLTSGRAQVVLSWWDIEMDPEGKIKCTMAPFWAHSDPEEMQWRDHWMQCVYFLPQEEPVVQGSALYLVAHHDDYCVWYSLQRTSPEKNERVRQMRPVCDCQAHLLWNRPRFGEINDQDRTDRYVQALRTVLKPDSVCLCVSDGSLLSVLAHHLGVEQVFTVESSAASHKLLRKIFKANHLEDKINIIEKRPELLTNEDLQGRKVSLLLGEPFFTTSLLPWHNLYFWYVRTAVDQHLGPGAMVMPQAASLHAVVVEFRDLWRIRSPCGDCEGFDVHIMDDMIKRALDFRESREAEPHPLWEYPCRSLSEPWQILTFDFQQPVPLQPLCAEGTVELRRPGQSHAAVLWMEYHLTPECTLSTGLLEPADPEGGCCWNPHCKQAVYFFSPAPDPRALLGGPRTVSYAVEFHPDTGDIIMEFRHADTPD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NVM4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K2 | Ubiquitination | Uniprot | |
| R7 | Methylation | Uniprot | |
| Y24 | Phosphorylation | Uniprot | |
| Y26 | Phosphorylation | Uniprot | |
| R32 | Methylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| T73 | Phosphorylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| C262 | S-Nitrosylation | Uniprot | |
| K444 | Methylation | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| R531 | Methylation | Uniprot | |
| K550 | Ubiquitination | Uniprot |
研究背景
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.
Cytoplasm>Cytosol. Nucleus.
Homodimer and heterodimer (By similarity). Interacts with CTCFL (By similarity). Interacts with PRMT5 and SNRPD3.
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.
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