产品: NODAL 抗体
货号: DF7791
描述: Rabbit polyclonal antibody to NODAL
应用: WB
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 40 kDa, 30 kDa; 40kD(Calculated).
蛋白号: Q96S42
RRID: AB_2841248

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
NODAL Antibody detects endogenous levels of total NODAL.
RRID:
AB_2841248
引用格式: Affinity Biosciences Cat# DF7791, RRID:AB_2841248.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

HTX5; MGC138230; Nodal; Nodal Growth Differentiation Factor; nodal homolog (mouse); Nodal homolog; Nodal, Mouse, Homolog; NODAL_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MHAHCLPFLLHAWWALLQAGAATVATALLRTRGQPSSPSPLAYMLSLYRDPLPRADIIRSLQAEDVAVDGQNWTFAFDFSFLSQQEDLAWAELRLQLSSPVDLPTEGSLAIEIFHQPKPDTEQASDSCLERFQMDLFTVTLSQVTFSLGSMVLEVTRPLSKWLKHPGALEKQMSRVAGECWPRPPTPPATNVLLMLYSNLSQEQRQLGGSTLLWEAESSWRAQEGQLSWEWGKRHRRHHLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGCL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Essential for mesoderm formation and axial patterning during embryonic development.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer; disulfide-linked.

蛋白家族:

Belongs to the TGF-beta family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

文献引用

1). The Photoperiod Regulates Granulosa Cell Apoptosis through the FSH-Nodal/ALK7 Signaling Pathway in Phodopus sungorus. Animals, 2022 (PubMed: 36552491) [IF=3.0]

Application: WB    Species: Mouse    Sample: Phodopus sungorus

Figure 7. Levels of the Nodal and ACVR1C protein expression in the ovaries of Phodopus sungorus under three photoperiod conditions. (A,B) Representative immunoblots of the key Nodal and ACVR1C proteins and internal reference proteins (α-tubulin and β-actin) in three different photoperiod groups. (C,D) Relative protein expression of Nodal and ACVR1C. Values are means ± SEM. n = 8. SD, short daylight; MD, moderate daylight; LD, long daylight.

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