产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7798, RRID:AB_2841253.
展开/折叠
ML12A_HUMAN; MLC 2B; MLC-2B; MLCB; MYL12A; Myosin regulatory light chain 12A; Myosin regulatory light chain 2; Myosin regulatory light chain 2, nonsarcomeric; Myosin regulatory light chain MRCL3; Myosin regulatory light chain MRLC3; Myosin RLC; Myosin, light polypeptide, regulatory, non sarcomeric (20kD); nonsarcomeric; RLC;
抗原和靶标
A synthesized peptide derived from human MYL12A, corresponding to a region within C-terminal amino acids.
- P19105 ML12A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD
翻译修饰 - P19105 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T10 | Phosphorylation | Uniprot | |
| T18 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| S19 | Phosphorylation | Q13464 (ROCK1) , P53355-3 (DAPK1) , Q13177 (PAK2) | Uniprot |
| S28 | Phosphorylation | Uniprot | |
| K50 | Acetylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| T127 | Phosphorylation | Uniprot | |
| R132 | Methylation | Uniprot | |
| T134 | Phosphorylation | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K150 | Sumoylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| Y155 | Phosphorylation | Uniprot | |
| K163 | Ubiquitination | Uniprot |
研究背景
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge (By similarity).
Myosin is a hexamer of 2 heavy chains and 4 light chains.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
限制条款
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