产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7813, RRID:AB_2841264.
展开/折叠
ADAP; Adhesion and degranulation promoting adaptor protein; FYB 120/130; Fyb; FYB-120/130; FYB_HUMAN; FYN binding protein; FYN T binding protein; FYN-binding protein; FYN-T-binding protein; p120/p130; PRO0823; SLAP 130; SLAP-130; SLAP130; SLP 76 associated phosphoprotein; SLP-76-associated phosphoprotein; SLP76 associated phosphoprotein;
抗原和靶标
Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.
- O15117 FYB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAKYNTGGNPTEDVSVNSRPFRVTGPNSSSGIQARKNLFNNQGNASPPAGPSNVPKFGSPKPPVAVKPSSEEKPDKEPKPPFLKPTGAGQRFGTPASLTTRDPEAKVGFLKPVGPKPINLPKEDSKPTFPWPPGNKPSLHSVNQDHDLKPLGPKSGPTPPTSENEQKQAFPKLTGVKGKFMSASQDLEPKPLFPKPAFGQKPPLSTENSHEDESPMKNVSSSKGSPAPLGVRSKSGPLKPAREDSENKDHAGEISSLPFPGVVLKPAASRGGPGLSKNGEEKKEDRKIDAAKNTFQSKINQEELASGTPPARFPKAPSKLTVGGPWGQSQEKEKGDKNSATPKQKPLPPLFTLGPPPPKPNRPPNVDLTKFHKTSSGNSTSKGQTSYSTTSLPPPPPSHPASQPPLPASHPSQPPVPSLPPRNIKPPFDLKSPVNEDNQDGVTHSDGAGNLDEEQDSEGETYEDIEASKEREKKREKEEKKRLELEKKEQKEKEKKEQEIKKKFKLTGPIQVIHLAKACCDVKGGKNELSFKQGEQIEIIRITDNPEGKWLGRTARGSYGYIKTTAVEIDYDSLKLKKDSLGAPSRPIEDDQEVYDDVAEQDDISSHSQSGSGGIFPPPPDDDIYDGIEEEDADDGFPAPPKQLDMGDEVYDDVDTSDFPVSSAEMSQGTNVGKAKTEEKDLKKLKKQEKEEKDFRKKFKYDGEIRVLYSTKVTTSITSKKWGTRDLQVKPGESLEVIQTTDDTKVLCRNEEGKYGYVLRSYLADNDGEIYDDIADGCIYDND
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15117 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K3 | Acetylation | Uniprot | |
| Y4 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| K111 | Acetylation | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K149 | Acetylation | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| T161 | Phosphorylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K179 | Acetylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| K265 | Acetylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| K334 | Acetylation | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| T374 | Phosphorylation | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Uniprot | |
| K425 | Acetylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| T461 | Phosphorylation | Uniprot | |
| Y462 | Phosphorylation | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| T507 | Phosphorylation | Uniprot | |
| K549 | Ubiquitination | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| Y559 | Phosphorylation | P06241 (FYN) | Uniprot |
| Y561 | Phosphorylation | Uniprot | |
| Y571 | Phosphorylation | P06241 (FYN) | Uniprot |
| S573 | Phosphorylation | Uniprot | |
| K575 | Ubiquitination | Uniprot | |
| Y595 | Phosphorylation | P06241 (FYN) | Uniprot |
| Y625 | Phosphorylation | P06241 (FYN) | Uniprot |
| Y651 | Phosphorylation | P06241 (FYN) | Uniprot |
| Y701 | Phosphorylation | Uniprot | |
| Y709 | Phosphorylation | Uniprot | |
| T711 | Phosphorylation | Uniprot | |
| K730 | Ubiquitination | Uniprot | |
| S734 | Phosphorylation | Uniprot | |
| K745 | Ubiquitination | Uniprot | |
| K754 | Ubiquitination | Uniprot | |
| Y755 | Phosphorylation | P06241 (FYN) | Uniprot |
| Y757 | Phosphorylation | P06241 (FYN) | Uniprot |
| Y762 | Phosphorylation | Uniprot | |
| Y771 | Phosphorylation | P06241 (FYN) | Uniprot |
| Y780 | Phosphorylation | P06241 (FYN) | Uniprot |
研究背景
Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells (By similarity). May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton. Modulates the expression of IL2 (By similarity). Involved in platelet activation (By similarity). Prevents the degradation of SKAP1 and SKAP2. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells (By similarity).
T-cell receptor ligation leads to increased tyrosine phosphorylation.
Cytoplasm. Nucleus. Cell junction.
Note: Colocalizes with TMEM47 at cell-cell contacts in podocytes.
Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.
Part of a complex consisting of SKAP2, FYB1 and PTPNS1 (By similarity). Part of a complex consisting of SKAP2, FYB1 and LILRB3 (By similarity). Part of a complex consisting of SKAP1, FYB1 and CLNK (By similarity). Interacts with CLNK (via its SH2 domain); this interaction allows SKAP1 and FYB1 to recruit FYN to the complex, thus promoting the phosphorylation of CLNK by FYN (By similarity). Interacts with FYN. Interacts with LCP2. Interacts with SKAP1. Interacts with SKAP2. Interacts with FASLG. Interacts with EVL. Interacts with TMEM47 (By similarity). Interacts with LCK.
研究领域
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
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