B-Myb Antibody - #DF7817

Figure 2 MALAT1 regulates downstream mRNA MYBL2 and confer PCa progression. (a) QRT-PCR verified the MYBL2 levels in MALAT1 knockdown PC-3 cell line (left panel) and C4-2 cell line (right panel). (b) Western blot examines MYBL2 level in MALAT1 knockdown PC-3 and C4-2 cell lines (left panel), and quantification (right panel) of western blot result. (c) QRT-PCR displayed the knockdown efficiency in PC-3 and C4-2 by treating with MYBL2 siRNA. (d) Western blot tests MYBL2 level after treating PC-3 and C4-2 cell line with siMYBL2 (left panel) and quantification (right panel) of western blot result. (e) QRT-PCR examines the MALAT1 level in knockdown MYBL2 PC-3 cell line (left panel) and in C4-2 (right panel). (f) Representative images of EdU assay formed by the vector control PC-3 cell line and MALAT1 or MYBL2 knockdown cell lines. Scale bars: 50 μm. (h) Representative images of colonies formed by vector control PC-3 cell line and MALAT1 or MYBL2 knockdown cell lines. (g and i) Quantification of EdU assay (left panel) and colonies (right panel). All samples were normalized to GAPDH mRNA and protein levels. The error bars indicate SD (n = 3). Student t test was used. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Figure 5 MALAT1/MYBL2/mTOR axis regulates PCa progression and glucose metabolism in mouse model. (a) The tumorigenic ability of nude mouse model (n = 6) between injected knockdown MALAT1 PC-3 cell siMALAT1 group and vector control NC group. (b) Final tumor weight between siMALAT1 group and NC group. (c) Tumor volume growth between two groups. (d) Representative images of hematoxylin-eosin staining between two groups. Scale bars: 100 μm. (e–i) Representative images of immunohistochemistry of Ki67 (e), MYBL2 (f), mTOR (g), p-mTOR (h), and PKM2 (i) between siMALAT1 group and NC group. (j) Quantification of IHC results. Scale bars: 100 μm. The error bars indicate SD (n = 6). Student t test was used. ∗P < 0.05, ∗∗P < 0.01.