| 产品: | Band 3/AE 1 抗体 |
| 货号: | DF7843 |
| 描述: | Rabbit polyclonal antibody to Band 3/AE 1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Horse, Rabbit, Dog |
| 分子量: | 102 kDa; 102kD(Calculated). |
| 蛋白号: | P02730 |
| RRID: | AB_2841281 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7843, RRID:AB_2841281.
展开/折叠
AE 1; AE1; Anion exchange protein 1; Anion exchanger 1; B3AT_HUMAN; Band 3 anion transport protein; BND3; CD233; DI; Diego blood group; EMPB3; EPB3; Erythrocyte membrane protein band 3; Erythroid anion exchange protein; FR; Froese blood group; RTA1A; SLC4A1; Solute carrier family 4 anion exchanger member 1; Solute carrier family 4 member 1; SW; Swann blood group; Waldner blood group; WD; WD1; WR; Wright blood group;
抗原和靶标
Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
- P02730 B3AT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEELQDDYEDMMEENLEQEEYEDPDIPESQMEEPAAHDTEATATDYHTTSHPGTHKVYVELQELVMDEKNQELRWMEAARWVQLEENLGENGAWGRPHLSHLTFWSLLELRRVFTKGTVLLDLQETSLAGVANQLLDRFIFEDQIRPQDREELLRALLLKHSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLFCEQGDGGTEGHSPSGILEKIPPDSEATLVLVGRADFLEQPVLGFVRLQEAAELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRIDAYMAQSRGELLHSLEGFLDCSLVLPPTDAPSEQALLSLVPVQRELLRRRYQSSPAKPDSSFYKGLDLNGGPDDPLQQTGQLFGGLVRDIRRRYPYYLSDITDAFSPQVLAAVIFIYFAALSPAITFGGLLGEKTRNQMGVSELLISTAVQGILFALLGAQPLLVVGFSGPLLVFEEAFFSFCETNGLEYIVGRVWIGFWLILLVVLVVAFEGSFLVRFISRYTQEIFSFLISLIFIYETFSKLIKIFQDHPLQKTYNYNVLMVPKPQGPLPNTALLSLVLMAGTFFFAMMLRKFKNSSYFPGKLRRVIGDFGVPISILIMVLVDFFIQDTYTQKLSVPDGFKVSNSSARGWVIHPLGLRSEFPIWMMFASALPALLVFILIFLESQITTLIVSKPERKMVKGSGFHLDLLLVVGMGGVAALFGMPWLSATTVRSVTHANALTVMGKASTPGAAAQIQEVKEQRISGLLVAVLVGLSILMEPILSRIPLAVLFGIFLYMGVTSLSGIQLFDRILLLFKPPKYHPDVPYVKRVKTWRMHLFTGIQIICLAVLWVVKSTPASLALPFVLILTVPLRRVLLPLIFRNVELQCLDADDAKATFDEEEGRDEYDEVAMPV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P02730 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| Y8 | Phosphorylation | P43405 (SYK) , P07948 (LYN) | Uniprot |
| Y21 | Phosphorylation | P43405 (SYK) , P07948 (LYN) | Uniprot |
| S29 | Phosphorylation | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| T42 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| T44 | Phosphorylation | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| S162 | O-Glycosylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| C201 | S-Nitrosylation | Uniprot | |
| S224 | O-Glycosylation | Uniprot | |
| S303 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| C317 | S-Nitrosylation | Uniprot | |
| Y347 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| Y359 | Phosphorylation | P07948 (LYN) , P43405 (SYK) | Uniprot |
| S525 | Phosphorylation | Uniprot | |
| N642 | N-Glycosylation | Uniprot | |
| S745 | O-Glycosylation | Uniprot | |
| K757 | Ubiquitination | Uniprot | |
| S781 | Phosphorylation | Uniprot | |
| Y818 | Phosphorylation | Uniprot | |
| T894 | Phosphorylation | Uniprot | |
| Y904 | Phosphorylation | P43405 (SYK) , P07948 (LYN) | Uniprot |
研究背景
Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
N-glycosylated.
Cell membrane>Multi-pass membrane protein. Basolateral cell membrane>Multi-pass membrane protein.
Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
Detected in erythrocytes (at protein level). Isoform 2 is expressed in kidney (at protein level).
A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association. Interacts with STOM. Isoform 2 interacts with TM139.
Belongs to the anion exchanger (TC 2.A.31) family.
研究领域
· Organismal Systems > Excretory system > Collecting duct acid secretion.
限制条款
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