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| 产品: | TRIB3 抗体 |
| 货号: | DF7844 |
| 描述: | Rabbit polyclonal antibody to TRIB3 |
| 应用: | WB IHC |
| 反应: | Human |
| 预测: | Pig, Bovine, Sheep |
| 分子量: | 40 kDa; 40kD(Calculated). |
| 蛋白号: | Q96RU7 |
| RRID: | AB_2841282 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7844, RRID:AB_2841282.
展开/折叠
C20orf97; Neuronal cell death inducible putative kinase; Neuronal cell death-inducible putative kinase; NIPK; p65 interacting inhibitor of NF-kappaB; p65-interacting inhibitor of NF-kappa-B; SINK; SKIP 3; SKIP3; TRB 3; TRB-3; TRB3; TRIB 3; Trib3; TRIB3_HUMAN; Tribbles homolog 3; Tribbles pseudokinase 3; Tribbles3;
抗原和靶标
Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
- Q96RU7 TRIB3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYQALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRGAYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVVLYG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96RU7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T4 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| T64 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| K219 | Ubiquitination | Uniprot |
研究背景
Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. Can inhibit APOBEC3A editing of nuclear DNA.
Nucleus.
Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
Interacts with AKT1, AKT2, ATF4, MAP2K1 and MAP2K7. Interacts with DDIT3/CHOP and inhibits its interaction with EP300/P300. Interacts (via N-terminus) with APOBEC3A. Interacts with APOBEC3C.
The protein kinase domain is predicted to be catalytically inactive.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
文献引用
Application: IHC Species: Human Sample: HCC cell
Application: IHC Species: Human Sample:
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