(1)
(7)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7850, RRID:AB_2841286.
展开/折叠
Early response protein NAK1; GFRP 1; GFRP; GFRP1; Growth factor inducible nuclear protein N10; Growth Factor Inducible Nuclear Protein NP10; Growth Factor Response Protein 1; Hbr1; HMR; Hormone Receptor; MGC9485; N10; N10 nuclear protein; NAK 1; NAK1; Nerve growth factor IB nuclear receptor variant 1; NGFIB; NP 10; NP10; NR4A1; NR4A1_HUMAN; Nuclear hormone receptor NUR/77; Nuclear Hormone Receptor TR3; Nuclear receptor subfamily 4 group A member 1; NUR77; NUR77, mouse, homolog of; Orphan nuclear receptor HMR; Orphan nuclear receptor NR4A1; Orphan nuclear receptor TR3; Orphan receptor tr3; Receptor NGFIB; ST 59; ST-59; ST59; Steroid receptor TR3; Testicular receptor 3; TR 3; TR3; TR3 orphan receptor;
抗原和靶标
- P22736 NR4A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPCIQAQYGTPAPSPGPRDHLASDPLTPEFIKPTMDLASPEAAPAAPTALPSFSTFMDGYTGEFDTFLYQLPGTVQPCSSASSSASSTSSSSATSPASASFKFEDFQVYGCYPGPLSGPVDEALSSSGSDYYGSPCSAPSPSTPSFQPPQLSPWDGSFGHFSPSQTYEGLRAWTEQLPKASGPPQPPAFFSFSPPTGPSPSLAQSPLKLFPSQATHQLGEGESYSMPTAFPGLAPTSPHLEGSGILDTPVTSTKARSGAPGGSEGRCAVCGDNASCQHYGVRTCEGCKGFFKRTVQKNAKYICLANKDCPVDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKQPPDASPANLLTSLVRAHLDSGPSTAKLDYSKFQELVLPHFGKEDAGDVQQFYDLLSGSLEVIRKWAEKIPGFAELSPADQDLLLESAFLELFILRLAYRSKPGEGKLIFCSGLVLHRLQCARGFGDWIDSILAFSRSLHSLLVDVPAFACLSALVLITDRHGLQEPRRVEELQNRIASCLKEHVAAVAGEPQPASCLSRLLGKLPELRTLCTQGLQRIFYLKLEDLVPPPPIIDKIFMDTLPF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P22736 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T10 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| T55 | Phosphorylation | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| K102 | Sumoylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S193 | Phosphorylation | Uniprot | |
| T196 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| K300 | Sumoylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| T339 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Q15418 (RPS6KA1) , P51812 (RPS6KA3) , O75582 (RPS6KA5) , P31749 (AKT1) | Uniprot |
| S360 | Phosphorylation | Uniprot | |
| K381 | Sumoylation | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| S431 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K558 | Ubiquitination | Uniprot |
研究背景
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Plays a role in the vascular response to injury (By similarity).
Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.
Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1.
Nucleus. Cytoplasm. Mitochondrion.
Note: Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27 (PubMed:22427340). Translocation to the mitochondrion upon interaction with RXRA and upon the presence of 9-cis retinoic acid (PubMed:17761950).
Fetal muscle and adult liver, brain and thyroid.
Binds DNA as a monomer (By similarity). Interacts with GADD45GIP1. Interacts with STK11. Interacts with IFI27. Heterodimer (via DNA-binding domain) with RXRA (via C-terminus); DNA-binding of the heterodimer is enhanced by 9-cis retinoic acid. Competes for the RXRA interaction with EP300 and thereby attenuates EP300 mediated acetylation of RXRA.
Belongs to the nuclear hormone receptor family. NR4 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
文献引用
Application: WB Species: Mouse Sample: lung tissues
限制条款
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