产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7883, RRID:AB_2841307.
展开/折叠
Adapter-related protein complex 2 sigma subunit; Adaptor protein complex AP 2 subunit sigma; Adaptor protein complex AP-2 subunit sigma; Adaptor protein complex AP2 subunit sigma; Adaptor related protein complex 2 sigma 1 subunit; AI043088; AP 17; AP 2 complex subunit sigma 1; AP-2 complex subunit sigma; AP17; AP17 delta; AP2 complex subunit sigma 1; Ap2s1; AP2S1_HUMAN; CLAPS 2; CLAPS2; Clathrin adaptor complex AP2, sigma subunit; Clathrin adaptor protein AP17; Clathrin assembly protein 2 small chain; Clathrin associated/assembly/adaptor protein small 2; Clathrin associated/assembly/adaptor protein small 2, 17-KD; Clathrin associated/assembly/adaptor protein, small 2 (17kD); Clathrin coat assembly protein AP17; Clathrin coat associated protein AP17; Clathrin coat-associated protein AP17; HA2 17 kDa subunit; MGC62945; Plasma membrane adaptor AP 2 17 kDa protein; Plasma membrane adaptor AP-2 17 kDa protein; Plasma membrane adaptor AP2 17 kDa protein; Sigma adaptin 3b; Sigma2 adaptin; Sigma2-adaptin;
抗原和靶标
- P53680 AP2S1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFCICVDVNDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQSLE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P53680 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K18 | Ubiquitination | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| S140 | Phosphorylation | Uniprot |
研究背景
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). May also play a role in extracellular calcium homeostasis.
Cell membrane. Membrane>Coated pit>Peripheral membrane protein>Cytoplasmic side.
Note: AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).
Belongs to the adaptor complexes small subunit family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.