MLC2 Antibody - #DF7911

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产品: MLC2 抗体
货号: DF7911
描述: Rabbit polyclonal antibody to MLC2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 19 kDa; 19kD(Calculated).
蛋白号: P10916
RRID: AB_2841328

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(92%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
MLC2 Antibody detects endogenous levels of total MLC2.
RRID:
AB_2841328
引用格式: Affinity Biosciences Cat# DF7911, RRID:AB_2841328.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cardiac myosin light chain-2; Cardiac ventricular myosin light chain 2; CMH10; MLC 2v; MLC-2; MLC-2v; MLC2; MLRV_HUMAN; MYL 2; MYL2; Myosin light chain 2 regulatory cardiac slow; Myosin light polypeptide 2 regulatory cardiac slow; Myosin regulatory light chain 2; Myosin regulatory light chain 2 ventricular/cardiac muscle isoform; Regulatory light chain of myosin; RLC of myosin; Slow cardiac myosin regulatory light chain 2; ventricular/cardiac muscle isoform;

抗原和靶标

免疫原:
Uniprot:

P10916(MLRV_HUMAN) >>Visit HPA database.

基因/基因ID:
MYL2(4633)
序列:
MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Bovine
92
Xenopus
92
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P10916 作为底物

Site PTM Type Enzyme
S15 Phosphorylation Q15746 (MYLK)
S19 Phosphorylation Q5VT25 (CDC42BPA)
T80 Phosphorylation
T98 Phosphorylation
K104 Acetylation
K111 Acetylation
Y118 Phosphorylation
T124 Phosphorylation
Y152 Phosphorylation
K153 Acetylation
K165 Acetylation

研究背景

功能:

Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).

翻译修饰:

N-terminus is methylated by METTL11A/NTM1.

Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity). Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity).

细胞定位:

Cytoplasm>Myofibril>Sarcomere>A band.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with MYOC.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes. Bioactive Materials, 2022 (PubMed: 35387166) [IF=18.9]

Application: WB    Species: rat    Sample: RAW 264.7 cells

Fig. S16. | Verification of transcriptome analysis of RAW 264.7 cells cultured on various scaffolds. The mRNA expression levels of (a) glycolytic and (b) RhoA/ROCK pathways-related genes in each group. (c) Western blot (WB) analysis of glycolytic and RhoA/ROCK pathways-related proteins HIF-1α, Pdk1, Pgk1, Cdc42, Rac1, and p-MLC/MLC in each group.
2). Protective effect of oxyberberine against acute lung injury in mice via inhibiting RhoA/ROCK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 35753262) [IF=7.5]
3). Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm-Induced Sudden Cardiac Death. International journal of molecular sciences, 2024 (PubMed: 38474189) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 4 Effects of CAS on the expression of phosphorylated MLC2 in rat coronary arteries. (A,B) Representative photomicrographs and semi-quantitative data of p-MLC2 protein levels detected via IHC staining. Lower enlarged images are from upper images. n = 6 per group. Scale bar: 100 µm (upper images) or 50 µm (lower images). (C) Representative immunofluores-cent images of p-MLC2 (red) and DAPI (blue) in the coronary arteries of rats. Scale bar: 50 µm. (D) The mean fluorescence intensity of p-MLC2 per section was quantified. n = 6 per group. (E,F) Western blot and quantitative analysis of p-MLC2 protein levels of rat coronary arteries. n = 6 per group. Data presented as mean ± SEM. ** p < 0.01 and *** p < 0.001 vs. control group.
4). An Inulin-Type Fructan CP-A from Codonopsis pilosula Alleviated 5-Fluorouracil-Induced Intestinal Mucositis via the ERK/MLCK/MLC2 Pathway and Regulation of Gut Microbiota. Pharmaceuticals, 2024 [IF=4.6]

Application: WB    Species: Rat    Sample: IEC-6 cells

Figure 4. Effects of CP-A on ERK/MLCK/MLC2 signaling pathway proteins in 5-FU-induced rats and IEC-6 cells: (a–d) Expression of MLC2, p-MLC2, MLCK, ERK1/2 and p-ERK1/2 in IEC-6 cells. (e) MLCK protein in IEC-6 cells (magnification 100×). (f–i) Expression of MLC2, p-MLC2, MLCK, ERK1/2 and p-ERK1/2 in intestinal. (j) The p-MLC2 protein in IEC-6 cells (magnification 100×). Values are represented as mean ± SEM (n = 3); ## p < 0.01 versus control group, * p < 0.05 and ** p < 0.01 versus 5-FU group.
5). High Glucose Aggravates Retinal Endothelial Cell Dysfunction by Activating the RhoA/ROCK1/pMLC/Connexin43 Signaling Pathway. Investigative Ophthalmology & Visual Science, 2022 (PubMed: 35881407) [IF=4.4]

Application: WB    Species: Mice    Sample: mRMVECs

Figure 6.RhoA/ROCK1 signaling regulates Cx43 through the directly interaction between pMLC and Cx43. (A, C) Co-immunoprecipitation of pMLC and Cx43 in mRMVECs with or without high glucose. (B, D, E) The protein expression of pMLC, MLC, and Cx43 in mRMVECs treated with different conditions. ns, no significance. EV, empty vector. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with the control group; ##P < 0.01 compared with the high glucose (30 mM) group. N = 3. The bars are the mean ± SD.
6). Somatostatin receptor 5 is critical for protecting intestinal barrier function in vivo and in vitro. Molecular and Cellular Endocrinology, 2021 (PubMed: 34224803) [IF=4.1]

Application: WB    Species: Mice    Sample: Colonic tissues

Fig. 8. L817,818 suppresses NF-κB-MLCK-MLC signaling in mice with DSS-induced colitis.
7). Inhibition of Peripheral ERK Signaling Ameliorates Persistent Muscle Pain Around Trigger Points in Rats. Cell Transplantation, 2020 (PubMed: 33081508) [IF=3.3]

Application: WB    Species: rat    Sample: muscle

Figure 6. |MLCK might be the target of the ERK inhibitor involved in ameliorating muscular mechanical hyperalgesia. (C) The expression of p-MLC protein. p-MLC expression was increased in the MPS group compared with that in the control group. p-MLC expression was decreased in the ERK inhibitor group compared with that in the MPS group.
8). Atractylenolide III suppresses senescence-associated secretome via inhibiting cGAS/NF-κB pathway in hepatic stellate cells. Clinical and experimental pharmacology & physiology, 2023 (PubMed: 36648378) [IF=2.9]
9). Dexmedetomidine Inhibited Peripheral Platelet-Derived Growth Factor Receptor Α Regulated Myofascial Trigger Points Pain and Muscle Fibers Contraction Via JAK2/STAT3 Signaling Pathway. , 2022
10). Calycosin-7-O-β-D-Glucoside Treatment Promotes Axonal Regeneration via Rho/ROCK Pathway After Ischemia/Reperfusion Injury. Research Square, 2020
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