产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7913, RRID:AB_2841329.
展开/折叠
Gamma 2; GAMMA-2; Gamma2; hWRS; IFI 53; IFI53; IFP 53; IFP53; Interferon induced protein 53; Interferon-induced protein 53; SYWC_HUMAN; T2-TrpRS; TrpRS; Tryptophan tRNA ligase, cytoplasmic; Tryptophan tRNA ligase 1 cytoplasmic; Tryptophan tRNA ligase; Tryptophan--tRNA ligase; Tryptophanyl tRNA synthetase; Tryptophanyl tRNA synthetase cytoplasmic; WARS; WARS protein; WRS;
抗原和靶标
- P23381 SYWC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKEVTDEIVKEFMTPRKLSFDFQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P23381 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K200 | Acetylation | Uniprot | |
| K204 | Acetylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| Y316 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| K366 | Acetylation | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| R381 | Methylation | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| K431 | Acetylation | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K465 | Ubiquitination | Uniprot | |
| S467 | Phosphorylation | Uniprot |
研究背景
Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
Cytoplasm.
Homodimer. Isoform 1 and isoform 2 interact with an oxidized form of GAPDH. GAPDH stimulates the aminoacylation activity of isoform 2.
Belongs to the class-I aminoacyl-tRNA synthetase family.
研究领域
· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.
限制条款
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