产品: PAX7 抗体
货号: DF7915
描述: Rabbit polyclonal antibody to PAX7
应用: WB
反应: Human
预测: Pig, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 57 kDa; 55kD(Calculated).
蛋白号: P23759
RRID: AB_2841330

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
PAX7 Antibody detects endogenous levels of total PAX7.
RRID:
AB_2841330
引用格式: Affinity Biosciences Cat# DF7915, RRID:AB_2841330.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ37460; HUP1; OTTHUMP00000002534; Paired box 7; Paired box gene 7; Paired box homeotic gene 7; Paired box protein Pax-7; Paired domain gene 7; Paired domain gene HuP1; Pax7; PAX7 transcriptional factor; PAX7/FKHR fusion gene, included; PAX7_HUMAN; PAX7B; RGD1564360; RMS2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MAALPGTVPRMMRPAPGQNYPRTGFPLEVSTPLGQGRVNQLGGVFINGRPLPNHIRHKIVEMAHHGIRPCVISRQLRVSHGCVSKILCRYQETGSIRPGAIGGSKPRQVATPDVEKKIEEYKRENPGMFSWEIRDRLLKDGHCDRSTVPSGLVSSISRVLRIKFGKKEEEDEADKKEDDGEKKAKHSIDGILGDKGNRLDEGSDVESEPDLPLKRKQRRSRTTFTAEQLEELEKAFERTHYPDIYTREELAQRTKLTEARVQVWFSNRRARWRKQAGANQLAAFNHLLPGGFPPTGMPTLPPYQLPDSTYPTTTISQDGGSTVHRPQPLPPSTMHQGGLAAAAAAADTSSAYGARHSFSSYSDSFMNPAAPSNHMNPVSNGLSPQVMSILGNPSAVPPQPQADFSISPLHGGLDSATSISASCSQRADSIKPGDSLPTSQAYCPPTYSTTGYSVDPVAGYQYGQYGQTAVDYLAKNVSLSTQRRMKLGEHSAVLGLLPVETGQAY

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Horse
0
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23759 作为底物

Site PTM Type Enzyme
Y121 Phosphorylation
T147 Phosphorylation
K176 Acetylation
S203 Phosphorylation
S207 Phosphorylation

研究背景

功能:

Transcription factor playing a role in myogenesis through regulation of muscle precursor cells proliferation.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Can bind to DNA as a heterodimer with PAX3. Interacts with PAXBP1; the interaction links PAX7 to a WDR5-containing histone methyltransferase complex (By similarity). Interacts with DAXX.

蛋白家族:

Belongs to the paired homeobox family.

研究领域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

文献引用

1). Sympathetic activity is correlated with satellite cell aging and myogenesis via β2-adrenoceptor. Stem Cell Research & Therapy, 2021 (PubMed: 34530910) [IF=7.5]

Application: IF/ICC    Species: Mice    Sample: SC cells

Fig. 2 The distributions of β-ADR and β2-ADR were decreased in aged SCs. A, B WB analysis showed low expression of β1-ADR, high expression of β2-ADR, and almost no expression of β3-ADR (n = 5) (F = 18.95, P < 0.001). C, D The β2-ADR expression in the SCs of aged mice was significantly lower than that in the SCs of young mice (n = 5) (t = 6.321, P < 0.001). E, F Immunofluorescence staining showed that the expression of β2-ADR in the TA muscles of aged mice was lower than that in the TA muscles of young mice (n = 8) (t = 1.31, P = 0.014). G–J Immunofluorescence staining showed that the expression of PAX7 was not significantly decreased in the SCs of aged mice in vitro (n = 8) (t = 0.18, P = 0.25) but that the number of PAX7− SCs (white arrow) in the proliferative phase was decreased significantly (n = 8) (t = 2.08, P = 0.007). The expression of β2-ADR was lower in aged mice than that in young mice (n = 8) (t = 1.67, P = 0.011). K The tissue concentration of NE was higher in young mice than in aged mice in both the noninjury and injury states (t = 3.07 and 2.97, P = 0.009 and 0.012). Independent samples t tests and one-way ANOVA with Tukey’s test, ns, not significant, *P < 0.05; **P < 0.001

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