(1)
(8)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7956, RRID:AB_2841354.
展开/折叠
Calcium release activated calcium channel protein 1; Calcium release activated calcium modulator 1; Calcium release-activated calcium channel protein 1; CRACM 1; CRACM1; CRCM1; CRCM1_HUMAN; FLJ14466; Orai 1; ORAI calcium release activated calcium modulator 1; orai1; ORAT1; Protein orai 1; Protein orai-1; TMEM 142A; TMEM142A; Transmembrane protein 142A;
抗原和靶标
- Q96D31 CRCM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96D31 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S19 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | P05771 (PRKCB) | Uniprot |
| S30 | Phosphorylation | P05771 (PRKCB) | Uniprot |
| S34 | Phosphorylation | Q13976 (PRKG1) | Uniprot |
| S65 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| T295 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| Y300 | Phosphorylation | Uniprot |
研究背景
Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
N-glycosylated.
Ubiquitinated.
Cys-195 is oxidated, leading to inactivate channel activity.
Cell membrane>Multi-pass membrane protein.
Note: Isoform beta is more mobile in the plasma membrane (PubMed:23307288). Colocalizes with STIM1 at the cell membrane (PubMed:27185316).
Interacts with STIM1 and STIM2; this regulates channel activity. Interacts with CALM; this may displace STIM1 and STIM2 and might thereby modulate channel activity. Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with CRACR2A/EFCAB4B and STIM1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Interacts with ASPH (isoform 8). Interacts with SLC35G1. Interacts with UBQLN1. Interacts with ADCY8; interaction is calcium store depletion independent; interaction occurs in membrane raft; interaction increases markedly after store depletion; positively regulates SOCE-induced adenylate cyclase activity; contributes to the targeting of ADCY8 to discrete regions of the plasma membrane that are shielded from other calcium events. Interacts with EFHB; the interaction takes place upon Ca(2+)-store depletion.
Belongs to the Orai family.
研究领域
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Endocrine system > Renin secretion.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
文献引用
Application: WB Species: Human Sample: HUVECs
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