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产品:	USP18 抗体
货号:	DF7968
描述:	Rabbit polyclonal antibody to USP18
应用:	WB
反应:	Human
分子量:	43 kDa.; 43kD(Calculated).
蛋白号:	Q9UMW8
RRID:	AB_2841360

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二抗
封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human

克隆:

Polyclonal

特异性:

USP18 Antibody detects endogenous levels of total USP18.

RRID:

AB_2841360
引用格式: Affinity Biosciences Cat# DF7968, RRID:AB_2841360.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

43 kDa ISG15 specific protease; 43 kDa ISG15-specific protease; EC 3.1.2.; hUBP43; Interferon Stimulated Gene 43 kD; ISG15 Specific Processing Protease; ISG15-specific-processing protease; ISG43; Ubiquitin Specific Peptidase 18; Ubiquitin Specific Protease 18 43 kD; Ubiquitin Specific Protease 18; Ubl carboxyl terminal hydrolase 18; Ubl carboxyl-terminal hydrolase 18; Ubl thioesterase 18; Ubl thiolesterase 18; Ubp15; UBP18_HUMAN; USP18;

抗原和靶标

免疫原:

Uniprot:

Q9UMW8(UBP18_HUMAN) >>Visit HPA database.

基因/基因ID:

USP18(11274)

序列:

Q9UMW8 UBP18_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNIGQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVPFQMLLLLEKMQDSRQKAVRPLELAYCLQKCNVPLFVQHDAAQLYLKLWNLIKDQITDVHLVERLQALYTIRVKDSLICVDCAMESSRNSSMLTLPLSLFDVDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGKQVLKLTHLPQTLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGGQYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQETAYLLVYMKMEC

翻译修饰 - Q9UMW8 作为底物

Q9UMW8

Site	PTM Type	Source
K3	Ubiquitination	Uniprot
Y125	Phosphorylation	Uniprot
K173	Ubiquitination	Uniprot
K268	Ubiquitination	Uniprot
K288	Ubiquitination	Uniprot

研究背景

Q9UMW8_UBP18_HUMAN

功能:

Involved in the regulation of inflammatory response to interferon type 1. Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.

Has enzymatic activity similar to isoform 1 and interferes with type I interferon signaling. Major deISGylation enzyme for nuclear proteins.

细胞定位:

Cytoplasm.

Nucleus. Cytoplasm.

蛋白家族:

Belongs to the peptidase C19 family.

文献引用

1). USP18‑deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal‑dependent manner. Oncology Letters, 2021 (PubMed: 33850562) [IF=2.9]

Application: IHC Species: Human Sample: tumor tissues

Figure 1. Downregulated USP18 expression in tumor tissues of a cervix tissue microarray. UCC tissues with grade (A) 1 and (B) 2 exhibited moderate staining, and grade (C) 3 exhibited weak staining of USP18. (D) Non-cancer tissues exhibited super intensive staining of USP18. Magnification, ×200. USP18, ubiquitin-specific peptidase 18.

Application: WB Species: Human Sample: HeLa cells

Figure 2. USP18 expression in HeLa cells. (A) Representative images of USP18 protein expression from western blot assay. (B) Fluorescence images of mock- and USP18-siRNA-transfected HeLa cells. Magnification, ×200. (C) Statistical analysis of USP18 protein expression. **P<0.01 vs. si-USP18. USP18, ubiquitin-specific peptidase 18; siRNA, small interfering RNA.

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USP18 Antibody - #DF7968

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