| 产品: | MBD1 抗体 |
| 货号: | DF7981 |
| 描述: | Rabbit polyclonal antibody to MBD1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Horse, Sheep, Rabbit, Dog |
| 分子量: | 67 kDa; 67kD(Calculated). |
| 蛋白号: | Q9UIS9 |
| RRID: | AB_2841368 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7981, RRID:AB_2841368.
展开/折叠
CXXC 3; CXXC-type zinc finger protein 3; CXXC3; MBD 1; Mbd1; MBD1_HUMAN; MECP1 COMPLEX; Methyl CpG binding domain protein 1; Methyl CpG binding domain protein 1 isoform PCM1; Methyl CpG binding protein MBD1; Methyl CpG binding protein splice variant 1; Methyl CpG binding protein splice variant 2; Methyl CpG binding protein splice variant 3; Methyl CpG binding protein splice variant 4; Methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; PCM 1; PCM1; Protein containing methyl-CpG-binding domain 1; Regulator of fibroblast growth factor 2 (FGF 2) transcription; RFT; The regulator of fibroblast growth factor 2(FGF 2) transcription;
抗原和靶标
- Q9UIS9 MBD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGILCYPAPKAHPVAVASKKRKKPSRPAKTRKRQVGPQSGEVRKEAPRDETKADTDTAPASFPAPGCCENCGISFSGDGTQRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECAACQVTEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTQEDCGHCPICLRPPRPGLRRQWKCVQRRCLRGKHARRKGGCDSKMAARRRPGAQPLPPPPPSQSPEPTEPHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGACAACLRRMDCGRCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWSESEDGAGSPPPYRRRKRPSSARRHHLGPTLKPTLATRTAQPDHTQAPTKQEAGGGFVLPPPGTDLVFLREGASSPVQVPGPVAASTEALLQEAQCSGLSWVVALPQVKQEKADTQDEWTPGTAVLTSPVLVPGCPSKAVDPGLPSVKQEPPDPEEDKEENKDDSASKLAPEEEAGGAGTPVITEIFSLGGTRFRDTAVWLPRSKDLKKPGARKQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UIS9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S37 | Phosphorylation | Uniprot | |
| K46 | Sumoylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| T116 | Phosphorylation | Uniprot | |
| T120 | Phosphorylation | Uniprot | |
| K277 | Sumoylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| Y403 | Phosphorylation | Uniprot | |
| K422 | Acetylation | Uniprot | |
| K422 | Sumoylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| K499 | Sumoylation | Uniprot | |
| K502 | Sumoylation | Uniprot | |
| K502 | Ubiquitination | Uniprot | |
| T505 | Phosphorylation | Uniprot | |
| K538 | Sumoylation | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| T570 | Phosphorylation | Uniprot | |
| T574 | Phosphorylation | Uniprot | |
| T587 | Phosphorylation | Uniprot | |
| R593 | Methylation | Uniprot | |
| K595 | Methylation | Uniprot |
研究背景
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
Nucleus. Nucleus matrix. Nucleus speckle. Chromosome.
Note: Colocalizes with the Ten-1 ICD form of TENM1 in foci associated with the nuclear matrix (By similarity). Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
Widely expressed.
Interacts with the Ten-1 ICD form of TENM1 (By similarity). Interacts with OASL, AFT7IP, AFT7IP2 and BAHD1. Binds CHAF1A and the SUV39H1-CBX5 complex via the MBD domain. Binds MGP via the TRD domain. May be part of the MeCP1 complex. During DNA replication, it recruits SETDB1 to form a S phase-specific complex that facilitates methylation of H3 'Lys-9' during replication-coupled chromatin assembly and is at least composed of the CAF-1 subunit CHAF1A, MBD1 and SETDB1.
The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
The third CXXC-type zinc finger mediates binding to DNA containing unmethylated CpG dinucleotides.
The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
限制条款
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