产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8000, RRID:AB_2841381.
展开/折叠
actinin 4; Actinin alpha 4; actinin4; ACTN 4; ACTN4; ACTN4_HUMAN; Alpha-actinin-4; DKFZp686K23158; F actin cross linking protein; F-actin cross-linking protein; Focal segmental glomerulosclerosis 1; FSGS 1; FSGS; FSGS1; Non muscle alpha actinin 4; Non-muscle alpha-actinin 4;
抗原和靶标
- O43707 ACTN4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43707 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y4 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| Y11 | Phosphorylation | Uniprot | |
| Y13 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| K50 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K114 | Acetylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| T163 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| Y212 | Phosphorylation | Uniprot | |
| K214 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K217 | Acetylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K233 | Acetylation | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| Y260 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| Y265 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| S269 | Phosphorylation | Uniprot | |
| R280 | Methylation | Uniprot | |
| K283 | Acetylation | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| R310 | Methylation | Uniprot | |
| T312 | Phosphorylation | Uniprot | |
| R319 | Methylation | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| K331 | Acetylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| Y338 | Phosphorylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| C351 | S-Nitrosylation | Uniprot | |
| T359 | Phosphorylation | Uniprot | |
| T362 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| Y397 | Phosphorylation | Uniprot | |
| K417 | Acetylation | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| K432 | Acetylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| K437 | Acetylation | Uniprot | |
| Y441 | Phosphorylation | Uniprot | |
| T445 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| K455 | Acetylation | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| R469 | Methylation | Uniprot | |
| Y485 | Phosphorylation | Uniprot | |
| Y486 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| T493 | Phosphorylation | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| C499 | S-Nitrosylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| Y533 | Phosphorylation | Uniprot | |
| K592 | Acetylation | Uniprot | |
| K592 | Ubiquitination | Uniprot | |
| K604 | Ubiquitination | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| Y611 | Phosphorylation | Uniprot | |
| T612 | Phosphorylation | Uniprot | |
| T615 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| K622 | Acetylation | Uniprot | |
| K622 | Ubiquitination | Uniprot | |
| K625 | Acetylation | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K632 | Ubiquitination | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| S656 | Phosphorylation | Uniprot | |
| T667 | Phosphorylation | Uniprot | |
| T682 | Phosphorylation | Uniprot | |
| Y693 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| Y700 | Phosphorylation | Uniprot | |
| K701 | Ubiquitination | Uniprot | |
| Y727 | Phosphorylation | Uniprot | |
| T741 | Phosphorylation | Uniprot | |
| T746 | Phosphorylation | Uniprot | |
| T756 | Phosphorylation | Uniprot | |
| K760 | Ubiquitination | Uniprot | |
| S763 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | Uniprot | |
| K779 | Acetylation | Uniprot | |
| K779 | Methylation | Uniprot | |
| K779 | Ubiquitination | Uniprot | |
| C793 | S-Nitrosylation | Uniprot | |
| S796 | Phosphorylation | Uniprot | |
| R805 | Methylation | Uniprot | |
| T839 | Phosphorylation | Uniprot | |
| T840 | Phosphorylation | Uniprot | |
| T842 | Phosphorylation | Uniprot | |
| T844 | Phosphorylation | Uniprot | |
| K853 | Acetylation | Uniprot | |
| K859 | Acetylation | Uniprot | |
| K859 | Ubiquitination | Uniprot | |
| R868 | Methylation | Uniprot | |
| Y878 | Phosphorylation | Uniprot | |
| Y898 | Phosphorylation | Uniprot | |
| K899 | Acetylation | Uniprot | |
| Y906 | Phosphorylation | Uniprot | |
| S909 | Phosphorylation | Uniprot |
研究背景
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.
Nucleus. Cytoplasm. Cell junction. Cytoplasm>Cytoskeleton>Stress fiber.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC-2, and bladder cancer KU-7).
Widely expressed.
Homodimer; antiparallel (By similarity). Binds TRIM3 at the N-terminus (By similarity). Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation (By similarity). Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 (By similarity). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with BAIAP1 and PDLIM2. Interacts with PPARG and RARA. Binds to VCL; this interaction triggers VCL conformational changes.
Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif that mediates interaction with nuclear receptors.
Belongs to the alpha-actinin family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Immune diseases > Systemic lupus erythematosus.
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.