| 产品: | FLII 抗体 |
| 货号: | DF8003 |
| 描述: | Rabbit polyclonal antibody to FLII |
| 应用: | WB |
| 反应: | Human |
| 预测: | Pig, Zebrafish, Bovine, Horse, Dog, Chicken, Xenopus |
| 分子量: | 145 kDa; 145kD(Calculated). |
| 蛋白号: | Q13045 |
| RRID: | AB_2841382 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8003, RRID:AB_2841382.
展开/折叠
Fli 1; FLI; Fli1; Flightless 1; Flightless I (Drosophila) homolog; Flightless I homolog; Flightless I homolog (Drosophila); Flightless1; FlightlessI; fliI; FLII_HUMAN; Fliih; FLIL; MGC39265; Protein flightless 1 homolog; Protein flightless-1 homolog;
抗原和靶标
Strongest expression in skeletal muscle with high expression also in the heart and lung.
- Q13045 FLII_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEATGVLPFVRGVDLSGNDFKGGYFPENVKAMTSLRWLKLNRTGLCYLPEELAALQKLEHLSVSHNNLTTLHGELSSLPSLRAIVARANSLKNSGVPDDIFKLDDLSVLDLSHNQLTECPRELENAKNMLVLNLSHNSIDTIPNQLFINLTDLLYLDLSENRLESLPPQMRRLVHLQTLVLNGNPLLHAQLRQLPAMTALQTLHLRSTQRTQSNLPTSLEGLSNLADVDLSCNDLTRVPECLYTLPSLRRLNLSSNQITELSLCIDQWVHVETLNLSRNQLTSLPSAICKLSKLKKLYLNSNKLDFDGLPSGIGKLTNLEEFMAANNNLELVPESLCRCPKLRKLVLNKNHLVTLPEAIHFLTEIEVLDVRENPNLVMPPKPADRAAEWYNIDFSLQNQLRLAGASPATVAAAAAAGSGPKDPMARKMRLRRRKDSAQDDQAKQVLKGMSDVAQEKNKKQEESADARAPSGKVRRWDQGLEKPRLDYSEFFTEDVGQLPGLTIWQIENFVPVLVEEAFHGKFYEADCYIVLKTFLDDSGSLNWEIYYWIGGEATLDKKACSAIHAVNLRNYLGAECRTVREEMGDESEEFLQVFDNDISYIEGGTASGFYTVEDTHYVTRMYRVYGKKNIKLEPVPLKGTSLDPRFVFLLDRGLDIYVWRGAQATLSSTTKARLFAEKINKNERKGKAEITLLVQGQELPEFWEALGGEPSEIKKHVPEDFWPPQPKLYKVGLGLGYLELPQINYKLSVEHKQRPKVELMPRMRLLQSLLDTRCVYILDCWSDVFIWLGRKSPRLVRAAALKLGQELCGMLHRPRHATVSRSLEGTEAQVFKAKFKNWDDVLTVDYTRNAEAVLQSPGLSGKVKRDAEKKDQMKADLTALFLPRQPPMSLAEAEQLMEEWNEDLDGMEGFVLEGKKFARLPEEEFGHFYTQDCYVFLCRYWVPVEYEEEEKKEDKEEKAEGKEGEEATAEAEEKQPEEDFQCIVYFWQGREASNMGWLTFTFSLQKKFESLFPGKLEVVRMTQQQENPKFLSHFKRKFIIHRGKRKAVQGAQQPSLYQIRTNGSALCTRCIQINTDSSLLNSEFCFILKVPFESEDNQGIVYAWVGRASDPDEAKLAEDILNTMFDTSYSKQVINEGEEPENFFWVGIGAQKPYDDDAEYMKHTRLFRCSNEKGYFAVTEKCSDFCQDDLADDDIMLLDNGQEVYMWVGTQTSQVEIKLSLKACQVYIQHMRSKEHERPRRLRLVRKGNEQHAFTRCFHAWSAFCKALA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13045 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| K21 | Acetylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| Y390 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| S418 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| S436 | Phosphorylation | Q96BR1 (SGK3) | Uniprot |
| K447 | Ubiquitination | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| K482 | Ubiquitination | Uniprot | |
| Y523 | Phosphorylation | Uniprot | |
| Y528 | Phosphorylation | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| Y657 | Phosphorylation | Uniprot | |
| K671 | Ubiquitination | Uniprot | |
| K678 | Ubiquitination | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K730 | Ubiquitination | Uniprot | |
| Y737 | Phosphorylation | Uniprot | |
| K746 | Ubiquitination | Uniprot | |
| K752 | Ubiquitination | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| S768 | Phosphorylation | Uniprot | |
| T772 | Phosphorylation | Uniprot | |
| S792 | Phosphorylation | Uniprot | |
| K802 | Ubiquitination | Uniprot | |
| T818 | Phosphorylation | Q96BR1 (SGK3) | Uniprot |
| S822 | Phosphorylation | Uniprot | |
| K832 | Methylation | Uniprot | |
| K832 | Ubiquitination | Uniprot | |
| K836 | Ubiquitination | Uniprot | |
| S856 | Phosphorylation | Uniprot | |
| S860 | Phosphorylation | Uniprot | |
| K862 | Ubiquitination | Uniprot | |
| K874 | Ubiquitination | Uniprot | |
| Y946 | Phosphorylation | Uniprot | |
| K951 | Ubiquitination | Uniprot | |
| K962 | Ubiquitination | Uniprot | |
| T968 | Phosphorylation | Uniprot | |
| K1007 | Ubiquitination | Uniprot | |
| K1015 | Ubiquitination | Uniprot | |
| K1029 | Ubiquitination | Uniprot | |
| K1035 | Ubiquitination | Uniprot | |
| K1046 | Ubiquitination | Uniprot | |
| Y1102 | Phosphorylation | Uniprot | |
| K1115 | Ubiquitination | Uniprot | |
| K1162 | Ubiquitination | Uniprot | |
| K1173 | Ubiquitination | Uniprot | |
| Y1175 | Phosphorylation | Uniprot | |
| Y1227 | Phosphorylation | Uniprot |
研究背景
May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
Nucleus. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cell junction>Focal adhesion.
Note: Colocalizes to actin-rich structures in blastocysts and, together with HRAS, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes (By similarity).
Strongest expression in skeletal muscle with high expression also in the heart and lung.
Interacts with actin, ACTL6A, NCOA2, CARM1 and MYD88. Interacts with LRRFIP1 and LRRFIP2. Upon LPS stimulation, LRRFIP2 competes for MYD88-binding. LRRFIP1 constitutively blocks the interaction with MyD88, even in the absence of LPS. Interacts with the nuclear receptors ESR1 and THRB. Interacts with SGK3.
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