产品: | UBA52 抗体 |
货号: | DF8021 |
描述: | Rabbit polyclonal antibody to UBA52 |
应用: | WB IF/ICC |
反应: | Human, Mouse, Monkey |
预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
分子量: | 15 kDa, 55kD(ubiquitinated); 15kD(Calculated). |
蛋白号: | P62987 |
RRID: | AB_2841392 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8021, RRID:AB_2841392.
展开/折叠
60S ribosomal protein L40; CEP52; HUBCEP52; L40; MGC126879; MGC57125; RPL40; UBA 52; Ubiquitin 52 amino acid fusion protein; Ubiquitin 60S ribosomal protein L40; Ubiquitin A 52 residue ribosomal protein fusion product 1; Ubiquitin carboxyl extension protein 52; Ubiquitin CEP52;
抗原和靶标
- P62987 RL40_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLRQLAQKYNCDKMICRKCYARLHPRAVNCRKKKCGHTNNLRPKKKVK
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P62987 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K6 | Acetylation | Uniprot | |
K6 | Methylation | Uniprot | |
K6 | Sumoylation | Uniprot | |
K6 | Ubiquitination | Uniprot | |
T7 | Phosphorylation | Uniprot | |
K11 | Acetylation | Uniprot | |
K11 | Sumoylation | Uniprot | |
K11 | Ubiquitination | Uniprot | |
T12 | Phosphorylation | Uniprot | |
T14 | Phosphorylation | Uniprot | |
S20 | Phosphorylation | Uniprot | |
T22 | Phosphorylation | Uniprot | |
K27 | Sumoylation | Uniprot | |
K27 | Ubiquitination | Uniprot | |
K29 | Sumoylation | Uniprot | |
K29 | Ubiquitination | Uniprot | |
K33 | Sumoylation | Uniprot | |
K33 | Ubiquitination | Uniprot | |
K48 | Acetylation | Uniprot | |
K48 | Sumoylation | Uniprot | |
K48 | Ubiquitination | Uniprot | |
S57 | Phosphorylation | Uniprot | |
Y59 | Phosphorylation | Uniprot | |
K63 | Methylation | Uniprot | |
K63 | Sumoylation | Uniprot | |
K63 | Ubiquitination | Uniprot | |
S65 | Phosphorylation | Uniprot | |
T66 | Phosphorylation | Uniprot | |
R72 | Methylation | Uniprot | |
S81 | Phosphorylation | Uniprot | |
K88 | Acetylation | Uniprot | |
K88 | Ubiquitination | Uniprot | |
C91 | S-Nitrosylation | Uniprot | |
K93 | Acetylation | Uniprot | |
K93 | Ubiquitination | Uniprot | |
K98 | Methylation | Uniprot | |
C115 | S-Nitrosylation | Uniprot |
研究背景
Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Component of the 60S subunit of the ribosome. Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs.
Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy. Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30.
Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.
Cytoplasm. Nucleus.
Cytoplasm.
Ribosomal protein L40 is part of the 60S ribosomal subunit. Interacts with UBQLN1 (via UBA domain).
In the N-terminal section; belongs to the ubiquitin family.
In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family.
研究领域
· Genetic Information Processing > Translation > Ribosome.
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