| 产品: | Ras-GRF1 抗体 |
| 货号: | DF8026 |
| 描述: | Rabbit polyclonal antibody to Ras-GRF1 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog, Xenopus |
| 分子量: | 145 kDa; 145kD(Calculated). |
| 蛋白号: | Q13972 |
| RRID: | AB_2841395 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8026, RRID:AB_2841395.
展开/折叠
CDC25; CDC25L; GNRP; GRF1; GRF55; Guanine nucleotide exchange factor; Guanine nucleotide releasing factor 55 kD; Guanine nucleotide releasing protein; guanine nucleotide-releasing factor 1; Guanine nucleotide-releasing protein; H GRF55; HGRF55; PP13187; Ras protein specific guanine nucleotide releasing factor 1; Ras specific guanine nucleotide releasing factor; Ras specific guanine nucleotide releasing factor CDC25 homolog; Ras specific nucleotide exchange factor CDC25; Ras-GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-specific nucleotide exchange factor CDC25; RASGRF1; RGRF1_HUMAN;
抗原和靶标
- Q13972 RGRF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEITSLLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKRTREGTREAEMSRSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTETSPTKSPTTPKSVKNKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13972 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S14 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| S481 | Phosphorylation | Uniprot | |
| S709 | Phosphorylation | Uniprot | |
| S730 | Phosphorylation | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| S752 | Phosphorylation | Uniprot | |
| S758 | Phosphorylation | Uniprot | |
| S787 | Phosphorylation | Uniprot | |
| S927 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T1023 | Phosphorylation | Uniprot | |
| S1090 | Phosphorylation | Uniprot | |
| S1095 | Phosphorylation | Uniprot | |
| S1252 | Phosphorylation | Uniprot |
研究背景
Promotes the exchange of Ras-bound GDP by GTP.
Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.
Ubiquitinated and degraded following phosphorylation by PLK2.
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.
Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability (By similarity).
The DH (DBL-homology) domain mediates interaction with RASGRF2.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
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