产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8034, RRID:AB_2841402.
展开/折叠
APG9 autophagy 9-like 1; APG9 like 1; APG9-like 1; APG9L1; ATG9; ATG9 autophagy related 9 homolog A; ATG9 autophagy related 9 homolog A (S. cerevisiae); ATG9A; ATG9A_HUMAN; Autophagy 9-like 1 protein; Autophagy related 9A; Autophagy related protein 9A; Autophagy-related protein 9A; mATG9; MGD3208; OTTHUMP00000206046; OTTHUMP00000206048; OTTHUMP00000206049; OTTHUMP00000206062;
抗原和靶标
- Q7Z3C6 ATG9A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQFDTEYQRLEASYSDSPPGEEDLLVHVAEGSKSPWHHIENLDLFFSRVYNLHQKNGFTCMLIGEIFELMQFLFVVAFTTFLVSCVDYDILFANKMVNHSLHPTEPVKVTLPDAFLPAQVCSARIQENGSLITILVIAGVFWIHRLIKFIYNICCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHKRELTELDIYHRILRFQNYMVALVNKSLLPLRFRLPGLGEAVFFTRGLKYNFELILFWGPGSLFLNEWSLKAEYKRGGQRLELAQRLSNRILWIGIANFLLCPLILIWQILYAFFSYAEVLKREPGALGARCWSLYGRCYLRHFNELEHELQSRLNRGYKPASKYMNCFLSPLLTLLAKNGAFFAGSILAVLIALTIYDEDVLAVEHVLTTVTLLGVTVTVCRSFIPDQHMVFCPEQLLRVILAHIHYMPDHWQGNAHRSQTRDEFAQLFQYKAVFILEELLSPIVTPLILIFCLRPRALEIIDFFRNFTVEVVGVGDTCSFAQMDVRQHGHPQWLSAGQTEASVYQQAEDGKTELSLMHFAITNPGWQPPRESTAFLGFLKEQVQRDGAAASLAQGGLLPENALFTSIQSLQSESEPLSLIANVVAGSSCRGPPLPRDLQGSRHRAEVASALRSFSPLQPGQAPTGRAHSTMTGSGVDARTASSGSSVWEGQLQSLVLSEYASTEMSLHALYMHQLHKQQAQAEPERHVWHRRESDESGESAPDEGGEGARAPQSIPRSASYPCAAPRPGAPETTALHGGFQRRYGGITDPGTVPRVPSHFSRLPLGGWAEDGQSASRHPEPVPEEGSEDELPPQVHKV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q7Z3C6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| Y8 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| Y15 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| N99 | N-Glycosylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y209 | Phosphorylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| Y545 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| R645 | Methylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S654 | Phosphorylation | Uniprot | |
| S656 | Phosphorylation | Uniprot | |
| S675 | Phosphorylation | Uniprot | |
| S735 | Phosphorylation | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| S741 | Phosphorylation | Uniprot | |
| S755 | Phosphorylation | Uniprot | |
| S759 | Phosphorylation | Uniprot | |
| S761 | Phosphorylation | Q13131 (PRKAA1) , O75385 (ULK1) | Uniprot |
| R784 | Methylation | Uniprot | |
| Y785 | Phosphorylation | Uniprot | |
| S799 | Phosphorylation | Uniprot | |
| S828 | Phosphorylation | Uniprot |
研究背景
Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.
Cytoplasmic vesicle>Autophagosome membrane>Multi-pass membrane protein. Golgi apparatus>trans-Golgi network membrane>Multi-pass membrane protein. Late endosome membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation-induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.
Interacts with SUPT20H.
Belongs to the ATG9 family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
限制条款
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