(1)
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产品描述
来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
TRIM29 Antibody detects endogenous levels of total TRIM29.
RRID:
AB_2841408
引用格式: Affinity Biosciences Cat# DF8050, RRID:AB_2841408.
引用格式: Affinity Biosciences Cat# DF8050, RRID:AB_2841408.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
Ataxia telangiectasia group D associated protein; Ataxia telangiectasia group D-associated protein; ATDC; FLJ36085; TRI29_HUMAN; TRIM 29; TRIM29; Tripartite motif containing 29; Tripartite motif containing protein 29; Tripartite motif protein 29; Tripartite motif protein TRIM29; Tripartite motif-containing protein 29;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
表达:
序列:
- Q14134 TRI29_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAADASRSNGSSPEARDARSPSGPSGSLENGTKADGKDAKTTNGHGGEAAEGKSLGSALKPGEGRSALFAGNEWRRPIIQFVESGDDKNSNYFSMDSMEGKRSPYAGLQLGAAKKPPVTFAEKGELRKSIFSESRKPTVSIMEPGETRRNSYPRADTGLFSRSKSGSEEVLCDSCIGNKQKAVKSCLVCQASFCELHLKPHLEGAAFRDHQLLEPIRDFEARKCPVHGKTMELFCQTDQTCICYLCMFQEHKNHSTVTVEEAKAEKETELSLQKEQLQLKIIEIEDEAEKWQKEKDRIKSFTTNEKAILEQNFRDLVRDLEKQKEEVRAALEQREQDAVDQVKVIMDALDERAKVLHEDKQTREQLHSISDSVLFLQEFGALMSNYSLPPPLPTYHVLLEGEGLGQSLGNFKDDLLNVCMRHVEKMCKADLSRNFIERNHMENGGDHRYVNNYTNSFGGEWSAPDTMKRYSMYLTPKGGVRTSYQPSSPGRFTKETTQKNFNNLYGTKGNYTSRVWEYSSSIQNSDNDLPVVQGSSSFSLKGYPSLMRSQSPKAQPQTWKSGKQTMLSHYRPFYVNKGNGIGSNEAP
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
90
Xenopus
70
Chicken
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14134 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| T33 | Phosphorylation | Uniprot | |
| K34 | Acetylation | Uniprot | |
| K38 | Acetylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| K102 | Acetylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| Y106 | Phosphorylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K116 | Acetylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| T139 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K275 | Acetylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| K291 | Acetylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K300 | Acetylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K361 | Acetylation | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| Y454 | Phosphorylation | Uniprot | |
| T467 | Phosphorylation | Uniprot | |
| K469 | Methylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| Y471 | Phosphorylation | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| Y474 | Phosphorylation | Uniprot | |
| T476 | Phosphorylation | Uniprot | |
| K478 | Acetylation | Uniprot | |
| Y485 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| K495 | Acetylation | Uniprot | |
| K495 | Ubiquitination | Uniprot | |
| K500 | Ubiquitination | Uniprot | |
| Y506 | Phosphorylation | Uniprot | |
| T508 | Phosphorylation | Uniprot | |
| K509 | Methylation | Uniprot | |
| K509 | Ubiquitination | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| Y544 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| S550 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| S552 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| K554 | Acetylation | Uniprot | |
| K554 | Ubiquitination | Uniprot | |
| K561 | Methylation | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| K564 | Ubiquitination | Uniprot | |
| Y575 | Phosphorylation | Uniprot |
研究背景
功能:
Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of proinflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation.
翻译修饰:
Constitutively phosphorylated by PKC on serine/threonine in A431 cells.
细胞定位:
Cytoplasm. Lysosome.
Note: Colocalizes with intermediate filaments.
组织特异性:
Expressed in placenta, prostate and thymus.
亚基结构:
Interacts with VIM and HINT1. Interacts with IKBKG/NEMO. Interacts with STING1.
文献引用
1). HCP5 prevents ubiquitination-mediated UTP3 degradation to inhibit apoptosis by activating c-Myc transcriptional activity. Molecular Therapy, 2022
(PubMed: 36245126)
[IF=12.1]
Application: WB Species: Human Sample: KYSE150 and KYSE510 cells
Figure 4 HCP5 stabilizes UTP3 by blocking the interaction between UTP3 and TRIM29 (A) List of UTP3-interacting E3 ligases identified using MS analysis in KYSE150 and KYSE510 cells. (B) Western blot analyses of TRIM29 and UTP3 expression in control and TRIM29-silenced (shT29#1 and shT29#2) KYSE150 and KYSE510 cells. Representative results of at least three biological replicates are shown. (C) Western blot analyses of TRIM29 and UTP3 expression in 293T cells transfected with increasing amounts of TRIM29 (0.5, 1.0, and 2.0 μg). Representative results of at least three biological replicates are shown. (D) Confocal microscopy assessment of UTP3 and TRIM29 colocalization in the indicated cells. Nuclei were stained with Hoechst 33342. Scale bars, 30 μm. (E and F) Western blot analyses of TRIM29 and UTP3 distribution after coIP assays with the indicated cells (C, cytoplasm; N, nucleus). Representative results of at least three biological replicates are shown. (G and H) Western blot analyses to assess UTP3 ubiquitination in control and TRIM29-silenced KYSE150 (G) and KYSE510 (H) cells. Representative results of at least three biological replicates are shown. (I) Western blot analyses of TRIM29 and UTP3 in coIP assays performed with control and HCP5-silenced KYSE150 and KYSE510 cells. Representative results of at least three biological replicates are shown. (J and K) Confocal microscopy assessment of PLA spots (red) in the indicated cells. Each spot indicates a single interaction between UTP3 and TRIM29 proteins. Nuclei were stained with DAPI (blue). Scale bars, 10 μm. The data are presented as the means ± SD; two-tailed t test, ∗∗∗p < 0.001, ∗∗p < 0.01; n = 3. (L) Western blot analyses to assess UTP3 ubiquitination in the indicated cells. Representative results of at least three biological replicates are shown.
Application: IF/ICC Species: Human Sample: KYSE150 and KYSE510 cells
Figure 4 HCP5 stabilizes UTP3 by blocking the interaction between UTP3 and TRIM29 (A) List of UTP3-interacting E3 ligases identified using MS analysis in KYSE150 and KYSE510 cells. (B) Western blot analyses of TRIM29 and UTP3 expression in control and TRIM29-silenced (shT29#1 and shT29#2) KYSE150 and KYSE510 cells. Representative results of at least three biological replicates are shown. (C) Western blot analyses of TRIM29 and UTP3 expression in 293T cells transfected with increasing amounts of TRIM29 (0.5, 1.0, and 2.0 μg). Representative results of at least three biological replicates are shown. (D) Confocal microscopy assessment of UTP3 and TRIM29 colocalization in the indicated cells. Nuclei were stained with Hoechst 33342. Scale bars, 30 μm. (E and F) Western blot analyses of TRIM29 and UTP3 distribution after coIP assays with the indicated cells (C, cytoplasm; N, nucleus). Representative results of at least three biological replicates are shown. (G and H) Western blot analyses to assess UTP3 ubiquitination in control and TRIM29-silenced KYSE150 (G) and KYSE510 (H) cells. Representative results of at least three biological replicates are shown. (I) Western blot analyses of TRIM29 and UTP3 in coIP assays performed with control and HCP5-silenced KYSE150 and KYSE510 cells. Representative results of at least three biological replicates are shown. (J and K) Confocal microscopy assessment of PLA spots (red) in the indicated cells. Each spot indicates a single interaction between UTP3 and TRIM29 proteins. Nuclei were stained with DAPI (blue). Scale bars, 10 μm. The data are presented as the means ± SD; two-tailed t test, ∗∗∗p < 0.001, ∗∗p < 0.01; n = 3. (L) Western blot analyses to assess UTP3 ubiquitination in the indicated cells. Representative results of at least three biological replicates are shown.
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