产品: HSPA14 抗体
货号: DF8060
描述: Rabbit polyclonal antibody to HSPA14
应用: WB IF/ICC
反应: Human, Monkey
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 55 kDa; 55kD(Calculated).
蛋白号: Q0VDF9
RRID: AB_2841416

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Monkey
预测:
Pig(100%), Bovine(80%), Horse(80%), Rabbit(91%), Dog(90%)
克隆:
Polyclonal
特异性:
HSPA14 Antibody detects endogenous levels of total HSPA14.
RRID:
AB_2841416
引用格式: Affinity Biosciences Cat# DF8060, RRID:AB_2841416.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Heat shock 70 kDa protein 14; Heat shock 70kDa protein 14; Heat shock protein HSP60; Heat shock protein hsp70 related protein; HSP60; HSP70 4; HSP70 like protein 1; HSP70-like protein 1; HSP70L1; HSP7E_HUMAN; HSPA 14; HSPA14; MGC131990;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MAAIGVHLGCTSACVAVYKDGRAGVVANDAGDRVTPAVVAYSENEEIVGLAAKQSRIRNISNTVMKVKQILGRSSSDPQAQKYIAESKCLVIEKNGKLRYEIDTGEETKFVNPEDVARLIFSKMKETAHSVLGSDANDVVITVPFDFGEKQKNALGEAARAAGFNVLRLIHEPSAALLAYGIGQDSPTGKSNILVFKLGGTSLSLSVMEVNSGIYRVLSTNTDDNIGGAHFTETLAQYLASEFQRSFKHDVRGNARAMMKLTNSAEVAKHSLSTLGSANCFLDSLYEGQDFDCNVSRARFELLCSPLFNKCIEAIRGLLDQNGFTADDINKVVLCGGSSRIPKLQQLIKDLFPAVELLNSIPPDEVIPIGAAIEAGILIGKENLLVEDSLMIECSARDILVKGVDESGASRFTVLFPSGTPLPARRQHTLQAPGSISSVCLELYESDGKNSAKEETKFAQVVLQDLDKKENGLRDILAVLTMKRDGSLHVTCTDQETGKCEAISIEIAS

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Rabbit
91
Dog
90
Horse
80
Bovine
80
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q0VDF9 作为底物

Site PTM Type Enzyme
K53 Ubiquitination
K66 Ubiquitination
K68 Ubiquitination
S75 Phosphorylation
S76 Phosphorylation
K82 Ubiquitination
S87 Phosphorylation
K88 Ubiquitination
Y100 Phosphorylation
K109 Ubiquitination
K123 Ubiquitination
K125 Ubiquitination
K150 Ubiquitination
S186 Phosphorylation
S219 Phosphorylation
K260 Acetylation
Y286 Phosphorylation
S305 Phosphorylation
K310 Ubiquitination
K331 Ubiquitination
K343 Ubiquitination
K402 Ubiquitination
T420 Phosphorylation
T456 Phosphorylation
K457 Ubiquitination
K468 Ubiquitination
K469 Acetylation
T481 Phosphorylation
S487 Phosphorylation

研究背景

功能:

Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.

细胞定位:

Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2.

蛋白家族:

Belongs to the heat shock protein 70 family.

限制条款

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