SLC7A5 Antibody - #DF8065

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产品: SLC7A5 抗体
货号: DF8065
描述: Rabbit polyclonal antibody to SLC7A5
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Sheep, Rabbit, Chicken, Xenopus
分子量: 55 kDa; 55kD(Calculated).
蛋白号: Q01650
RRID: AB_2841420

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(91%), Zebrafish(82%), Bovine(82%), Sheep(82%), Rabbit(82%), Chicken(82%), Xenopus(82%)
克隆:
Polyclonal
特异性:
SLC7A5 Antibody detects endogenous levels of total SLC7A5.
RRID:
AB_2841420
引用格式: Affinity Biosciences Cat# DF8065, RRID:AB_2841420.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

4F2 LC; 4F2 light chain; 4F2LC; CD98; CD98 light chain; CD98LC; D16S469E; DC49; E16; hLAT1; Integral membrane protein E16; L type amino acid transporter; L type amino acid transporter 1; L-type amino acid transporter 1; Large neutral amino acids transporter 1; Large neutral amino acids transporter; Large neutral amino acids transporter small subunit 1; LAT1; LAT1_HUMAN; Membrane protein E16; MPE16; Slc7a5; Sodium independent neutral amino acid transporter; Sodium independent neutral amino acid transporter LAT1; Solute carrier family 7 (cationic amino acid transporter y+ system) member 5; Solute carrier family 7 member 5; TA1; Tumor associated protein 1; y+ system cationic amino acid transporter;

抗原和靶标

免疫原:
Uniprot:

Q01650(LAT1_HUMAN) >>Visit HPA database.

基因/基因ID:
SLC7A5(8140)
表达:
Q01650 LAT1_HUMAN:

Detected in placenta, in the syncytiotrophoblast layer (at protein level) (PubMed:11389679). Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.

序列:
MAGAGPKRRALAAPAAEEKEEAREKMLAAKSADGSAPAGEGEGVTLQRNITLLNGVAIIVGTIIGSGIFVTPTGVLKEAGSPGLALVVWAACGVFSIVGALCYAELGTTISKSGGDYAYMLEVYGSLPAFLKLWIELLIIRPSSQYIVALVFATYLLKPLFPTCPVPEEAAKLVACLCVLLLTAVNCYSVKAATRVQDAFAAAKLLALALIILLGFVQIGKGDVSNLDPNFSFEGTKLDVGNIVLALYSGLFAYGGWNYLNFVTEEMINPYRNLPLAIIISLPIVTLVYVLTNLAYFTTLSTEQMLSSEAVAVDFGNYHLGVMSWIIPVFVGLSCFGSVNGSLFTSSRLFFVGSREGHLPSILSMIHPQLLTPVPSLVFTCVMTLLYAFSKDIFSVINFFSFFNWLCVALAIIGMIWLRHRKPELERPIKVNLALPVFFILACLFLIAVSFWKTPVECGIGFTIILSGLPVYFFGVWWKNKPKWLLQGIFSTTVLCQKLMQVVPQET

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
91
Bovine
82
Sheep
82
Xenopus
82
Zebrafish
82
Chicken
82
Rabbit
82
Horse
73
Dog
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q01650 作为底物

Site PTM Type Enzyme
K7 Ubiquitination
K19 Ubiquitination
K25 Ubiquitination
K30 Ubiquitination
S31 Phosphorylation
S35 Phosphorylation
T45 Phosphorylation
S225 Phosphorylation
S354 Phosphorylation
T507 Phosphorylation

研究背景

功能:

The heterodimer with SLC3A2 functions as sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, L-DOPA, leucine, histidine, methionine and tryptophan. Functions as an amino acid exchanger. May play a role in the transport of L-DOPA across the blood-brain barrier (By similarity). May act as the major transporter of tyrosine in fibroblasts (Probable). May mediate blood-to-retina L-leucine transport across the inner blood-retinal barrier (By similarity). Can mediate the transport of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. When associated with LAPTM4B, the heterodimer formed by SLC3A2 and SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation. Involved in the uptake of toxic methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the membrane.

细胞定位:

Apical cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Lysosome membrane>Multi-pass membrane protein.
Note: Located to the plasma membrane by SLC3A2/4F2hc (PubMed:9751058). Localized to the apical membrane of placental syncytiotrophoblastic cells (PubMed:11742812). Recruited to lysosomes by LAPTM4B (PubMed:25998567). Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in placenta, in the syncytiotrophoblast layer (at protein level). Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2.

亚基结构:

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Interacts with LAPTM4B; this recruits the heterodimer formed by SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation.

蛋白家族:

Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

研究领域

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

文献引用

1). Resetting amino acid metabolism of cancer cells by ATB0,+-targeted nanoparticles for enhanced anticancer therapy. Bioactive Materials, 2022 (PubMed: 34820552) [IF=18.9]
2). Aurka deficiency in the intestinal epithelium promotes age-induced obesity via propionate-mediated AKT activation. International Journal of Biological Sciences, 2021 (PubMed: 33867847) [IF=9.2]

Application: WB    Species: Mice    Sample: intestines

Figure 4 p-AKT promoted lipid metabolism and nutrient absorption in the intestine of Aurkaf/+;VillinCre/+ mice. (A, B) Lipid metabolism- and nutrient absorption-related mRNA and protein levels of were measured in the intestines of Aurkaf/+;VillinCre/+ mice. The graphs show the mean ± SEM of one of three independent experiments. (n=3 mice per group). *, P < 0.05. **, P <0.01. (C, D, E, F) The relative length and width of crypts and villi were measured by ImageJ software. The histogram shows the mean ± SEM. (G) The ratio of villi to crypt length in the intestine. (H, I) The concentrations of IL-6 and IL-1β in the serum were measured by ELISA. The bar graphs show the mean ± SEM of one of two independent experiments that included five groups of mice. *P <0.05. (J, K) H&E staining and CD3 immunostaining of the intestines of Aurkaf/+and Aurkaf/+;VillinCre/+ mice. The quantitative data are shown on the right.
3). The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers. Poultry Science, 2023 (PubMed: 36680861) [IF=4.4]

Application: WB    Species: broilers    Sample: jejunum

Figure 1 Representative immunoblots of ZnT1, ZnT4, ZnT5, ZnT7, ZnT9, ZIP3, ZIP5, B0AT1, LAT1, y+LAT2, rBAT and PepT1 in the jejunum of broilers at 28 (A) and 39 d (B) of age. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y+LAT2, y+L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; ZnS, Zn sulfate; Zn-Prot M, Zn proteinate with moderate chelation strength (Qf = 51.6).
4). Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers. Frontiers in Physiology, 2022 (PubMed: 35936908) [IF=4.0]

Application: WB    Species: broilers    Sample:

FIGURE 3 Effect of dietary Zn source on the protein expression levels of Zn, amino acid, and small peptide transporters in the duodenum of broilers at 28 days of age. Values are means ± SE, (n = 7/8). Lacking the same letters (a, b, and c) means significant differences, p < 0.05. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B0AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y + LAT2, y + L-type amino acid transporter 2; rBAT, b0,+-type amino acid transporter; PepT1, peptide-transporter 1; Zn-Prot M, Zn proteinate with moderate chelation strength (Qf = 51.6). The protein expression levels were calculated as the relative quantities (RQs) of the target gene protein to the β-tubulin protein.
5). Zinc proteinate with moderate chelation strength enhances zinc absorption by up-regulating the expression of zinc and amino acid transporters in primary cultured duodenal epithelial cells of broiler embryos. Journal of animal science, 2024 (PubMed: 39031082) [IF=3.3]

Application: WB    Species: broiler    Sample:

Figure 2. Effect of Zn source (added Zn level of 400 μmol/L) on protein expression levels of ZnT, ZIP, amino acid, and small peptide transporters in primary cultured duodenal epithelial cells at 120 min of incubation (experiment 3). Values are means ± SE (n = 5 to 6). Lacking the same letters (a, b, c) means significant differences (P 
6). Metabolic Evaluation of MYCN-Amplified Neuroblastoma by 4-[18F]FGln PET Imaging. Molecular Imaging and Biology, 2019 (PubMed: 30850970) [IF=3.1]

Application: WB    Species: mouse    Sample: shASCT2 and shControl cells and tumor tissues

Fig. 4. |The uptake of 4-[18F]FGln reduced in ASCT2 knockdown tumors and cells and analysis of ASCT2 and LAT1 proteins after shRNA knockdown. a 4-[18F]FGln uptake in shASCT2-IMR-32 showed lower uptake than that in shControl-IMR-32, while[18F]FDG uptake showed no significant difference during the 120 min. b Relative mRNA expression of ASCT2 was calculated by 2−△△Ct method, and a reduced expression was observed after shASCT2 knockdown; ASCT2 expression showed a significant difference between in shASCT2 and shControl cells and tumor tissues. cASCT2 knockdown had no significant effect on the expression of LAT1 protein.
7). Self-assembled nanoparticles with bilirubin/JPH203 alleviate imiquimod-induced psoriasis by reducing oxidative stress and suppressing Th17 expansion. Chemical Engineering Journal, 2022

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