产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0183, RRID:AB_2833376.
展开/折叠
10 kDa chaperonin; 10 kDa heat shock protein mitochondrial; 10 kDa heat shock protein, mitochondrial; CH10_HUMAN; Chaperonin 10; Chaperonin 10 homolog; CPN10; cpn10 homolog; Early pregnancy factor; Early-pregnancy factor; EPF; GROES; GroES homolog; Heat shock 10kD protein 1 chaperonin 10; Heat shock 10kDa protein 1; Heat shock 10kDa protein 1 chaperonin 10; Heat-shock 10-kD protein; Hsp10; Hspe1;
抗原和靶标
- P61604 CH10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61604 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K8 | Acetylation | Uniprot | |
| K8 | Methylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| R15 | Methylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| K36 | Methylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| K40 | Acetylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| T45 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K56 | Acetylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K70 | Acetylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| Y76 | Phosphorylation | Uniprot | |
| T79 | Phosphorylation | Uniprot | |
| K80 | Acetylation | Uniprot | |
| K80 | Sumoylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K86 | Acetylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| Y88 | Phosphorylation | Uniprot | |
| R92 | Methylation | Uniprot | |
| K99 | Acetylation | Uniprot | |
| K99 | Ubiquitination | Uniprot |
研究背景
Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Mitochondrion matrix.
Homoheptamer arranged in a ring structure. 2 heptameric Hsp10 rings interact with a Hsp60 tetradecamer in the structure of a back-to-back double heptameric ring to form the symmetrical football complex.
Belongs to the GroES chaperonin family.
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