PBX3 Antibody - #DF8080

Fig. 7 MiR-224-5p binds to PBX3 to regulate AML cell activity. a Binding sequences between miR-224-5p and PBX3 were shown, and their binding effects were evaluated using dual luciferase reporter assay. b, c Western blotting was used to show relative protein levels of PBX3 in HL-60 and KG-1 cells infected with LV-miR-224-5p sponge and LV-miR-224-5p. HL-60 and KG-1 were then co-infected with the LV-miR-224-5p and LV-PBX3. d, e CCK-8 and flow cytometry was performed to detect the cell viability and cell apoptosis, respectively. f Relative protein levels of PBX3, Ki67, PCNA, BCL-2, cleaved caspase-3, ERK, and p-ERK in two co-infected AML cells were evaluated using western blotting. g Quantified figures of above protein levels from western blotting. h PBX3 protein expression was assessed in HL-60 and KG-1 cells after co-infection of LV-CD27-AS1 and LV-miR-224-5p by western blotting, with their quantification. N = 3. Data were shown as means ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001, and ns = no significance.