| 产品: | EVL 抗体 |
| 货号: | DF8091 |
| 描述: | Rabbit polyclonal antibody to EVL |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Dog |
| 分子量: | 50 kDa; 45kD(Calculated). |
| 蛋白号: | Q9UI08 |
| RRID: | AB_2841440 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8091, RRID:AB_2841440.
展开/折叠
AI528774; Ena-vasodilator stimulated phosphoprotein; Ena/vasodilator stimulated phosphoprotein like; Ena/VASP like protein; EVL; RNB 6; RNB6;
抗原和靶标
- Q9UI08 EVL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTASNTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNIMNSQEGGPSSQRQVQNGPSPDEMDIQRRQVMEQHQQQRQESLERRTSATGPILPPGHPSSAASAPVSCSGPPPPPPPPVPPPPTGATPPPPPPLPAGGAQGSSHDESSMSGLAAAIAGAKLRRVQRPEDASGGSSPSGTSKSDANRASSGGGGGGLMEEMNKLLAKRRKAASQSDKPAEKKEDESQMEDPSTSPSPGTRAASQPPNSSEAGRKPWERSNSVEKPVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALDAFDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UI08 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y16 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| T343 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| K399 | Ubiquitination | Uniprot |
研究背景
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.
Phosphorylated by PKA; phosphorylation abolishes binding to SH3 domains of ABL and SRC.
Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Stress fiber. Cell projection>Lamellipodium.
Note: Targeted to the leading edge of lamellipodia and the distal tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F-actin collar and the distal tip of microspikes.
Homotetramer (By similarity). Binds to the SH3 domains of ABL1, LYN and SRC. Also binds to profilin, with preference for isoform IIa of PFN2, and the WW domain of APBB1/FE65. Binds to SEMA6A. Interacts, via the Pro-rich region, with the C-terminal SH3 domain of DNMBP. Interacts with RAPH1. Binds, via the EVH1 domain, the Pro-rich domain of Listeria monocytogenes actA (By similarity). Binds, via the EVH1 domain, the Pro-rich domain of ZYX. Interacts with FYB1. Interacts with ZDHHC17.
The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
Belongs to the Ena/VASP family.
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