XDH Antibody - #DF8111

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产品: XDH 抗体
货号: DF8111
描述: Rabbit polyclonal antibody to XDH
应用: WB IHC
反应: Human, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 146 kDa; 146kD(Calculated).
蛋白号: P47989
RRID: AB_2841454

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Rat
预测:
Pig(88%), Bovine(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(88%)
克隆:
Polyclonal
特异性:
XDH Antibody detects endogenous levels of total XDH.
RRID:
AB_2841454
引用格式: Affinity Biosciences Cat# DF8111, RRID:AB_2841454.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Xanthine dehydrogenase; Xanthine dehydrogenase/oxidase; Xanthine oxidase; Xanthine oxidoreductase; XD; XDH; XDH_HUMAN; xdha; XO; xor;

抗原和靶标

免疫原:
Uniprot:

P47989(XDH_HUMAN) >>Visit HPA database.

基因/基因ID:
XDH(7498)
表达:
P47989 XDH_HUMAN:

Detected in milk (at protein level).

序列:
MTADKLVFFVNGRKVVEKNADPETTLLAYLRRKLGLSGTKLGCGEGGCGACTVMLSKYDRLQNKIVHFSANACLAPICSLHHVAVTTVEGIGSTKTRLHPVQERIAKSHGSQCGFCTPGIVMSMYTLLRNQPEPTMEEIENAFQGNLCRCTGYRPILQGFRTFARDGGCCGGDGNNPNCCMNQKKDHSVSLSPSLFKPEEFTPLDPTQEPIFPPELLRLKDTPRKQLRFEGERVTWIQASTLKELLDLKAQHPDAKLVVGNTEIGIEMKFKNMLFPMIVCPAWIPELNSVEHGPDGISFGAACPLSIVEKTLVDAVAKLPAQKTEVFRGVLEQLRWFAGKQVKSVASVGGNIITASPISDLNPVFMASGAKLTLVSRGTRRTVQMDHTFFPGYRKTLLSPEEILLSIEIPYSREGEYFSAFKQASRREDDIAKVTSGMRVLFKPGTTEVQELALCYGGMANRTISALKTTQRQLSKLWKEELLQDVCAGLAEELHLPPDAPGGMVDFRCTLTLSFFFKFYLTVLQKLGQENLEDKCGKLDPTFASATLLFQKDPPADVQLFQEVPKGQSEEDMVGRPLPHLAADMQASGEAVYCDDIPRYENELSLRLVTSTRAHAKIKSIDTSEAKKVPGFVCFISADDVPGSNITGICNDETVFAKDKVTCVGHIIGAVVADTPEHTQRAAQGVKITYEELPAIITIEDAIKNNSFYGPELKIEKGDLKKGFSEADNVVSGEIYIGGQEHFYLETHCTIAVPKGEAGEMELFVSTQNTMKTQSFVAKMLGVPANRIVVRVKRMGGGFGGKETRSTVVSTAVALAAYKTGRPVRCMLDRDEDMLITGGRHPFLARYKVGFMKTGTVVALEVDHFSNVGNTQDLSQSIMERALFHMDNCYKIPNIRGTGRLCKTNLPSNTAFRGFGGPQGMLIAECWMSEVAVTCGMPAEEVRRKNLYKEGDLTHFNQKLEGFTLPRCWEECLASSQYHARKSEVDKFNKENCWKKRGLCIIPTKFGISFTVPFLNQAGALLHVYTDGSVLLTHGGTEMGQGLHTKMVQVASRALKIPTSKIYISETSTNTVPNTSPTAASVSADLNGQAVYAACQTILKRLEPYKKKNPSGSWEDWVTAAYMDTVSLSATGFYRTPNLGYSFETNSGNPFHYFSYGVACSEVEIDCLTGDHKNLRTDIVMDVGSSLNPAIDIGQVEGAFVQGLGLFTLEELHYSPEGSLHTRGPSTYKIPAFGSIPIEFRVSLLRDCPNKKAIYASKAVGEPPLFLAASIFFAIKDAIRAARAQHTGNNVKELFRLDSPATPEKIRNACVDKFTTLCVTGVPENCKPWSVRV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Pig
88
Sheep
88
Chicken
88
Zebrafish
75
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P47989 作为底物

Site PTM Type Enzyme
T39 Phosphorylation
T222 Phosphorylation Q00535 (CDK5)
T311 Phosphorylation
T324 Phosphorylation
K443 Methylation
K552 Acetylation
S810 Phosphorylation
Y818 Phosphorylation
T856 Phosphorylation
S1052 Phosphorylation
S1111 Phosphorylation
S1113 Phosphorylation
T1320 Phosphorylation

研究背景

功能:

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).

翻译修饰:

Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).

Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).

细胞定位:

Cytoplasm. Peroxisome. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in milk (at protein level).

亚基结构:

Homodimer. Interacts with BTN1A1 (By similarity).

蛋白家族:

Belongs to the xanthine dehydrogenase family.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Biosynthesis of other secondary metabolites > Caffeine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Sesamol supplementation alleviates nonalcoholic steatohepatitis and atherosclerosis in high-fat, high carbohydrate and high-cholesterol diet-fed rats. Food & Function, 2021 (PubMed: 34606548) [IF=6.1]

Application: WB    Species: Rat    Sample: liver tissue

Fig. 6 Sesamol decreased uric acid levels through the inhibition of XO activity and/or its expression in HF-HCC diet-fed rats. (A–D) The analyses of serum and hepatic XO activities and uric acid levels (samples from 6 rats in each group were analyzed). (E) Representative images of the western blot analysis from hepatic XO expression, and (F) representative images of the IHC analyses from hepatic XO expression (samples from 3 rats in each group were analyzed). Values are expressed as means ± SEM (n = 6); #P < 0.05, # #P < 0.01 and ###P < 0.001, versus control; *P < 0.05 and **P < 0.01, versus HF-HCC.

Application: IHC    Species: Rat    Sample: liver tissue

Fig. 6 Sesamol decreased uric acid levels through the inhibition of XO activity and/or its expression in HF-HCC diet-fed rats. (A–D) The analyses of serum and hepatic XO activities and uric acid levels (samples from 6 rats in each group were analyzed). (E) Representative images of the western blot analysis from hepatic XO expression, and (F) representative images of the IHC analyses from hepatic XO expression (samples from 3 rats in each group were analyzed). Values are expressed as means ± SEM (n = 6); #P < 0.05, # #P < 0.01 and ###P < 0.001, versus control; *P < 0.05 and **P < 0.01, versus HF-HCC.

Application: WB    Species: Rat    Sample:

Fig. 6 Sesamol decreased uric acid levels through the inhibition of XO activity and/or its expression in HF-HCC diet-fed rats. (A–D) The analyses of serum and hepatic XO activities and uric acid levels (samples from 6 rats in each group were analyzed). (E) Representative images of the western blot analysis from hepatic XO expression, and (F) representative images of the IHC analyses from hepatic XO expression (samples from 3 rats in each group were analyzed). Values are expressed as means ± SEM (n = 6); # P < 0.05, # # P < 0.01 and # # # P < 0.001, versus control; *P < 0.05 and **P < 0.01, versus HF-HCC.
2). C1QBP regulates apoptosis of renal cell carcinoma via modulating xanthine dehydrogenase (XDH) mediated ROS generation. International Journal of Medical Sciences, 2022 (PubMed: 35693733) [IF=3.6]

Application: WB    Species: Human    Sample: ACHN and 786-O cells

Figure 2 C1QBP regulates the expression of XDH in RCC. (A) Western blot detected the expression of XDH in ACHN and 786-O cells with stable C1QBP knockdown (left panel) and C1QBP overexpression (right panel). (B) The XDH mRNA was examined by qRT-PCR in ACHN (upper panel) and 786-O (lower panel) cells with stable C1QBP knockdown (left panel) and C1QBP overexpression (right panel). The expression of C1QBP and XDH was detected by western blot both in tumors (T) and adjacent normal tissues (N) of 30 primary RCC patients. (C) Representative 8 pairs of western blot images were shown. (D) The expression of C1QBP (left panel) and XDH (right panel) was normalized with β-actin and quantified by Image J software in 30 primary RCC patients. The analysis was used paired t-test, P = 0.015, P < 0.0001, respectively, n = 30. (E) The expression of C1QBP and XDH was examined by immunohistochemistry staining in a study cohort with 57 pairs of RCC (Tumor) and adjacent normal kidney tissues (Para-tumor). Representative pictures were shown, and brown signals indicated positive staining. Scale bar = 50μm. Statistically significant differences were indicated: *, P < 0.05, **, P < 0.01 and ***, P < 0.001.

Application: IHC    Species: Human    Sample: kidney tissues

Figure 2 C1QBP regulates the expression of XDH in RCC. (A) Western blot detected the expression of XDH in ACHN and 786-O cells with stable C1QBP knockdown (left panel) and C1QBP overexpression (right panel). (B) The XDH mRNA was examined by qRT-PCR in ACHN (upper panel) and 786-O (lower panel) cells with stable C1QBP knockdown (left panel) and C1QBP overexpression (right panel). The expression of C1QBP and XDH was detected by western blot both in tumors (T) and adjacent normal tissues (N) of 30 primary RCC patients. (C) Representative 8 pairs of western blot images were shown. (D) The expression of C1QBP (left panel) and XDH (right panel) was normalized with β-actin and quantified by Image J software in 30 primary RCC patients. The analysis was used paired t-test, P = 0.015, P < 0.0001, respectively, n = 30. (E) The expression of C1QBP and XDH was examined by immunohistochemistry staining in a study cohort with 57 pairs of RCC (Tumor) and adjacent normal kidney tissues (Para-tumor). Representative pictures were shown, and brown signals indicated positive staining. Scale bar = 50μm. Statistically significant differences were indicated: *, P < 0.05, **, P < 0.01 and ***, P < 0.001.

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