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| 产品: | PLK4 抗体 |
| 货号: | DF8120 |
| 描述: | Rabbit polyclonal antibody to PLK4 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 95 kDa; 109kD(Calculated). |
| 蛋白号: | O00444 |
| RRID: | AB_2841460 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8120, RRID:AB_2841460.
展开/折叠
PLK 4; PLK-4; PLK4; PLK4_HUMAN; Polo like Kinase 4; Polo-like kinase 4; Protein serine/threonine kinase; SAK; Serine/Threonine Kinase 18; Serine/threonine kinase; Serine/threonine protein kinase 18; Serine/threonine protein kinase PLK4; Serine/threonine protein kinase Sak; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase PLK4; Serine/threonine-protein kinase SAK; Snk Akin Kinase; STK 18; STK18;
抗原和靶标
- O00444 PLK4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVEDSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERYSPTDNNANIFNFFKEKTSSSSGSFERPDNNQALSNHLCPGKTPFPFADPTPQTETVQQWFGNLQINAHLRKTTEYDSISPNRDFQGHPDLQKDTSKNAWTDTKVKKNSDASDNAHSVKQQNTMKYMTALHSKPEIIQQECVFGSDPLSEQSKTRGMEPPWGYQNRTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELVKEYASQEYVKEVLQISSDGNTITIYYPNGGRGFPLADRPPSPTDNISRYSFDNLPEKYWRKYQYASRFVQLVRSKSPKITYFTRYAKCILMENSPGADFEVWFYDGVKIHKTEDFIQVIEKTGKSYTLKSESEVNSLKEEIKMYMDHANEGHRICLALESIISEEERKTRSAPFFPIIIGRKPGSTSSPKALSPPPSVDSNYPTRERASFNRMVMHSAASPTQAPILNPSMVTNEGLGLTTTASGTDISSNSLKDCLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPNGQTTRYGENEKLPDYIKQKLQCLSSILLMFSNPTPNFH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O00444 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| S22 | Phosphorylation | O00444 (PLK4) | Uniprot |
| K45 | Acetylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | O00444 (PLK4) | Uniprot |
| T174 | Phosphorylation | Uniprot | |
| Y177 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S255 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S285 | Phosphorylation | O00444 (PLK4) | Uniprot |
| T289 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S291 | Phosphorylation | O00444 (PLK4) | Uniprot |
| T292 | Phosphorylation | O00444 (PLK4) | Uniprot |
| T295 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S297 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S298 | Phosphorylation | O00444 (PLK4) | Uniprot |
| T300 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S301 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S303 | Phosphorylation | O00444 (PLK4) | Uniprot |
| S305 | Phosphorylation | O00444 (PLK4) | Uniprot |
| K321 | Ubiquitination | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| R357 | Methylation | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| Y394 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| Y420 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| Y495 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| K538 | Acetylation | Uniprot | |
| T547 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| Y582 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| T591 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| S671 | Phosphorylation | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| T751 | Phosphorylation | Uniprot | |
| K753 | Ubiquitination | Uniprot | |
| S817 | Phosphorylation | Uniprot | |
| S821 | Phosphorylation | Uniprot |
翻译修饰 - O00444 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O00444 (PLK4) | S22 | Uniprot |
| O00444 (PLK4) | T170 | Uniprot |
| O00444 (PLK4) | Y177 | Uniprot |
| O00444 (PLK4) | S282 | Uniprot |
| O00444 (PLK4) | S285 | Uniprot |
| O00444 (PLK4) | T289 | Uniprot |
| O00444 (PLK4) | S291 | Uniprot |
| O00444 (PLK4) | T292 | Uniprot |
| O00444 (PLK4) | T295 | Uniprot |
| O00444 (PLK4) | S297 | Uniprot |
| O00444 (PLK4) | S298 | Uniprot |
| O00444 (PLK4) | T300 | Uniprot |
| O00444 (PLK4) | S301 | Uniprot |
| O00444 (PLK4) | S303 | Uniprot |
| O00444 (PLK4) | S305 | Uniprot |
| O96004 (HAND1) | T107 | Uniprot |
| O96004 (HAND1) | S109 | Uniprot |
| Q15154 (PCM1) | S372 | Uniprot |
| Q969U6 (FBXW5) | S151 | Uniprot |
| Q96RT7 (TUBGCP6) | S392 | Uniprot |
| Q96RT7 (TUBGCP6) | S1041 | Uniprot |
| Q96RT7 (TUBGCP6) | S1060 | Uniprot |
| Q96RT7 (TUBGCP6) | S1068 | Uniprot |
| Q96RT7 (TUBGCP6) | S1087 | Uniprot |
| Q96RT7 (TUBGCP6) | S1095 | Uniprot |
| Q96RT7 (TUBGCP6) | S1114 | Uniprot |
| Q96RT7 (TUBGCP6) | S1122 | Uniprot |
| Q96RT7 (TUBGCP6) | S1141 | Uniprot |
| Q96RT7 (TUBGCP6) | S1149 | Uniprot |
| Q96RT7 (TUBGCP6) | S1168 | Uniprot |
| Q96RT7 (TUBGCP6) | S1176 | Uniprot |
| Q96RT7 (TUBGCP6) | S1195 | Uniprot |
| Q96RT7 (TUBGCP6) | S1203 | Uniprot |
| Q96RT7 (TUBGCP6) | S1222 | Uniprot |
| Q96RT7 (TUBGCP6) | S1230 | Uniprot |
| Q96RT7 (TUBGCP6) | S1249 | Uniprot |
| Q96RT7 (TUBGCP6) | S1257 | Uniprot |
| Q96RT7 (TUBGCP6) | S1437 | Uniprot |
| Q96RT7 (TUBGCP6) | S1465 | Uniprot |
| Q9HC77 (CENPJ) | S595 | Uniprot |
研究背景
Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification.
Acetylation by KAT2A and KAT2B impairs kinase activity by shifting the kinase to an inactive conformation.
Ubiquitinated; leading to its degradation by the proteasome.
Tyrosine-phosphorylated by TEC.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Nucleus>Nucleolus. Cleavage furrow.
Note: Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
Homodimer (By similarity). Interacts with CEP152 (via N-terminus). Interacts with CEP78; this interaction may be important for proper PLK4 localization to the centriole and PLK4-induced overduplication of centrioles.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
研究领域
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
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