HE4 Antibody - #DF8160

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产品: HE4 抗体
货号: DF8160
描述: Rabbit polyclonal antibody to HE4
应用: WB IF/ICC
反应: Human, Mouse
预测: Dog
分子量: 23 kDa; 13kD(Calculated).
蛋白号: Q14508
RRID: AB_2841484

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:200-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Dog(86%)
克隆:
Polyclonal
特异性:
HE4 Antibody detects endogenous levels of total HE4.
RRID:
AB_2841484
引用格式: Affinity Biosciences Cat# DF8160, RRID:AB_2841484.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

dJ461P17.6; EDDM4; epididymal protein 4; Epididymal secretory protein E4; Epididymis specific whey acidic protein type four disulfide core; HE 4; Major epididymis specific protein E4; Major epididymis-specific protein E4; MGC57529; Putative protease inhibitor WAP5; WAP 5; WAP domain containing protein HE4; WAP domain containing protein HE4 V4; WAP four disulfide core domain 2; WAP four disulfide core domain protein 2; WAP four-disulfide core domain protein 2; WAP5; WFDC 2; WFDC2; WFDC2_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q14508(WFDC2_HUMAN) >>Visit HPA database.

基因/基因ID:
WFDC2(10406)
表达:
Q14508 WFDC2_HUMAN:

Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.

序列:
MPACRLGPLAAALLLSLLLFGFTLVSGTGAEKTGVCPELQADQNCTQECVSDSECADNLKCCSAGCATFCSLPNDKEGSCPQVNINFPQLGLCRDQCQVDSQCPGQMKCCRNGCGKVSCVTPNF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
86
Rabbit
75
Pig
71
Horse
71
Sheep
57
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14508 作为底物

Site PTM Type Enzyme
N44 N-Glycosylation

研究背景

功能:

Broad range protease inhibitor.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.

亚基结构:

Homotrimer; disulfide-linked.

文献引用

1). ZNF703 promotes tumor progression in ovarian cancer by interacting with HE4 and epigenetically regulating PEA15. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2020 (PubMed: 33246486) [IF=11.3]

Application: WB    Species: human    Sample: ovarian cancer cells

Fig. 4 ZNF703 interacted with HE4 and HE4 promoted the nuclear translocation of the ZNF703. a The co-immunoprecipitation analysis of ZNF703 and HE4 was performed in ovarian cancer cells (The left two were performed in CAOV3 cell line and the right two were performed in OVCAR3 cell line. The corresponding molecular weight was added to the right side).

Application: IF/ICC    Species: human    Sample: CAOV3 and OVCAR3 cells

Fig.4 The immunofluorescence co-localization of ZNF703 and HE4 was performed in CAOV3 and OVCAR3 cells (The green color was for ZNF703 and the red color was for HE4, DAPI was blue, the colocalization was yellow) (×800). The arrow indicated the co-localization in the nucleus.

Application: WB    Species: human    Sample: CAOV3 and OVCAR3 cells

Fig.4 e, f Western blot determined nuclear and cytoplasmic distribution of ZNF703 after nuclear cytoplasmic fractionation in CAOV3 and OVCAR3 cells transfected with the overexpressed HE4 plasmid or control.

Application: WB    Species: human    Sample: CAOV3 and OVCAR3 cells

Fig.4 e, f Western blot determined nuclear and cytoplasmic distribution of ZNF703 after nuclear cytoplasmic fractionation in CAOV3 and OVCAR3 cells transfected with the overexpressed HE4 plasmid or control.

Application: WB    Species: human    Sample: CAOV3 and OVCAR3 cells

Fig.4 g, h Nuclear and cytoplasmic distribution of ZNF703 tested by western blot after nuclear cytoplasmic fractionation in CAOV3 and OVCAR3 cells transfected with HE4 siRNA or control.

Application: WB    Species: human    Sample: CAOV3 and OVCAR3 cells

Fig.4 g, h Nuclear and cytoplasmic distribution of ZNF703 tested by western blot after nuclear cytoplasmic fractionation in CAOV3 and OVCAR3 cells transfected with HE4 siRNA or control.
2). YWHAE as an HE4 interacting protein can influence the malignant behaviour of ovarian cancer by regulating the PI3K/AKT and MAPK pathways. Cancer Cell International, 2021 (PubMed: 34107979) [IF=5.8]

Application: IHC    Species: Human    Sample: CAOV3 and ES2 cells

Fig. 1 YWHAE and HE4 are interacting proteins in ovarian cancer. a Expression of YWHAE and HE4 in CAOV3 and ES2 cells. b Immunofluorescence assay to detect the co-localisation of YWHAE and HE4. c Co-IP detection of interactions between YWHAE and HE4 in CAOV3 (left) and ES2 (right) cell lines. The first channel shows the Co-IP, the second shows IgG, and the third channel shows total protein. d, e Western blotting was used to detect the relationship between YWHAE and HE4. f Bioinformatics analysis showed that HE4 acts as an interaction factor for YWHAE

Application: IF/ICC    Species: Human    Sample: CAOV3 and ES2 cells

Fig. 1 YWHAE and HE4 are interacting proteins in ovarian cancer. a Expression of YWHAE and HE4 in CAOV3 and ES2 cells. b Immunofluorescence assay to detect the co-localisation of YWHAE and HE4. c Co-IP detection of interactions between YWHAE and HE4 in CAOV3 (left) and ES2 (right) cell lines. The first channel shows the Co-IP, the second shows IgG, and the third channel shows total protein. d, e Western blotting was used to detect the relationship between YWHAE and HE4. f Bioinformatics analysis showed that HE4 acts as an interaction factor for YWHAE

Application: WB    Species: Human    Sample: CAOV3 and ES2 cells

Fig. 1 YWHAE and HE4 are interacting proteins in ovarian cancer. a Expression of YWHAE and HE4 in CAOV3 and ES2 cells. b Immunofluorescence assay to detect the co-localisation of YWHAE and HE4. c Co-IP detection of interactions between YWHAE and HE4 in CAOV3 (left) and ES2 (right) cell lines. The first channel shows the Co-IP, the second shows IgG, and the third channel shows total protein. d, e Western blotting was used to detect the relationship between YWHAE and HE4. f Bioinformatics analysis showed that HE4 acts as an interaction factor for YWHAE

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