BNIP3L Antibody - #DF8163

Figure 6 BA attenuates mitophagy and reduces ROS accumulation after SCI. (A) ELISA of 8-OHdG, AOPP, and MDA in spinal cord lesions from Sham, SCI, BA and BA+3MA groups as indicated. (B) Immunofluorescence staining for Nix and NeuN co-localization in the spinal cords of the Sham, SCI, BA and BA+3MA groups (scale bar = 25 µm). (C) The quantitative mean optical density of the Nix in motor neurons of spinal cord lesion in each group. (D) Western blotting for Bnip3, Nix and Parkin expression levels in the Sham, SCI and BA groups. The gels were run under the same experimental conditions, and the cropped blots are shown here. (E) The optical density values of the Bnip3, Nix and Parkin expression levels were quantified and analyzed in the three groups. (F) Western blotting for Bnip3, Nix and Parkin expression levels in the BA and BA+3MA groups. The gels were run under the same experimental conditions, and the cropped blots are shown here. (G) The optical density values of the Bnip3, Nix and Parkin expression levels were quantified and analyzed in the both groups. The values are expressed as the means ± SEM, n=5 per group. *p< 0.05 and **p< 0.01, vs. Sham group. #p< 0.05 and ##p< 0.01, vs. SCI group. &p< 0.05 and &&p< 0.01, vs. BA group.

Fig. 9. Effects of SG formula on predicted targets expression of HFD induced - NASH model. (A-F) Representative western blot images and bar graphs of the relative expressions of BNIP3, BNIP3L, LC3BI, LC3BII and p62 in each group. * , * *, and * ** represent P 

Figure 6 Bexarotene promotes mitophagy and decreases ROS levels after SCI. (A) Immunofluorescence staining for GSDMD (pyroptosis-related marker, green), C-CASP1 (pyroptosis-related marker, red), NIX (mitophagy-related marker, red) and DHE (indicating ROS-positive cells, red) in neurons in the spinal cord (original magnification 30×). Scale bar: 25 μm. (B–E) Quantitative analysis of levels of GSDMD (B), C-CASP1 (C), NIX (D) and DHE (E) in A. (F–H) The levels of 8-OHdG and AOPP in the spinal cord were detected by ELISA, and the levels of MDA were detected by the thiobarbituric acid assay. (I, L) Western blot assay for pyroptosis-related and mitophagy-related proteins. Data were normalized to GAPDH. (M) The levels of mitophagy-related genes in the spinal cord were detected by qPCR and normalized to β-actin. Data are expressed as the mean ± SEM (n = 6 mice per group). *P < 0.05 and **P < 0.01, vs. SCI group; #P < 0.05 and ##P < 0.01, vs. SCI + Bex group (one-way analysis of variance with the least significance difference post hoc test). ASC: Apoptosis-associated speck-like protein containing a CARD; Bex: bexarotene; BNIP3: BCL2/adenovirus E1B 19 kDa interacting protein 3; C-CASP-1: cleaved Caspase 1; DAPI: 4′,6-diamidino-2-phenylindole; DHE: dihydroethidium; ELISA: enzyme-linked immunosorbent assay; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GSDMD-N: gasdermin D-N; IOD: integrated optical density; MDA: malondialdehyde; NIX/BNIP3L: BCL2/adenovirus E1B 19 kDa interacting protein 3 like; NLRP3: NOD-like receptor thermal protein domain associated protein 3; SCI: spinal cord injury.

Figure 6 Bexarotene promotes mitophagy and decreases ROS levels after SCI. (A) Immunofluorescence staining for GSDMD (pyroptosis-related marker, green), C-CASP1 (pyroptosis-related marker, red), NIX (mitophagy-related marker, red) and DHE (indicating ROS-positive cells, red) in neurons in the spinal cord (original magnification 30×). Scale bar: 25 μm. (B–E) Quantitative analysis of levels of GSDMD (B), C-CASP1 (C), NIX (D) and DHE (E) in A. (F–H) The levels of 8-OHdG and AOPP in the spinal cord were detected by ELISA, and the levels of MDA were detected by the thiobarbituric acid assay. (I, L) Western blot assay for pyroptosis-related and mitophagy-related proteins. Data were normalized to GAPDH. (M) The levels of mitophagy-related genes in the spinal cord were detected by qPCR and normalized to β-actin. Data are expressed as the mean ± SEM (n = 6 mice per group). *P < 0.05 and **P < 0.01, vs. SCI group; #P < 0.05 and ##P < 0.01, vs. SCI + Bex group (one-way analysis of variance with the least significance difference post hoc test). ASC: Apoptosis-associated speck-like protein containing a CARD; Bex: bexarotene; BNIP3: BCL2/adenovirus E1B 19 kDa interacting protein 3; C-CASP-1: cleaved Caspase 1; DAPI: 4′,6-diamidino-2-phenylindole; DHE: dihydroethidium; ELISA: enzyme-linked immunosorbent assay; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GSDMD-N: gasdermin D-N; IOD: integrated optical density; MDA: malondialdehyde; NIX/BNIP3L: BCL2/adenovirus E1B 19 kDa interacting protein 3 like; NLRP3: NOD-like receptor thermal protein domain associated protein 3; SCI: spinal cord injury.

Figure 6 Immunoblotting analysis of mitophagy proteins in liver tissue of control and hyperthyroid rats. (A) Representative Western blot of Parkin, PINK1, SQSTM1/p62, LC3A/B-I:II, BNIP3L, C1-C5-CR, T1-T5-HR. (B–F) Relative levels of appropriate proteins with respect to the loading control (GAPDH). CR, control; HR, hyperthyroidism. **—p < 0.02, ***—p < 0.001 as compared with the control data (n = 5–6).