产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0184, RRID:AB_2833377.
展开/折叠
60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; CH60_HUMAN; Chaperonin 60; Chaperonin, 60-KD; CPN60; fa04a05; GROEL; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 1 (chaperonin); Heat shock protein 60; Heat shock protein 65; heat shock protein family D (Hsp60) member 1; HLD4; Hsp 60; HSP 65; HSP-60; HSP60; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13;
抗原和靶标
- P10809 CH60_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGAMGGMGGGMGGGMF
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P10809 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
R3 | Methylation | Uniprot | |
K31 | Acetylation | Uniprot | |
K31 | Ubiquitination | Uniprot | |
K58 | Ubiquitination | Uniprot | |
T61 | Phosphorylation | Uniprot | |
S67 | Phosphorylation | Uniprot | |
S70 | Phosphorylation | Uniprot | |
K72 | Ubiquitination | Uniprot | |
T74 | Phosphorylation | Uniprot | |
K75 | Ubiquitination | Uniprot | |
T79 | Phosphorylation | Uniprot | |
K82 | Acetylation | Uniprot | |
K82 | Ubiquitination | Uniprot | |
S83 | Phosphorylation | Uniprot | |
K87 | Acetylation | Uniprot | |
K87 | Ubiquitination | Uniprot | |
Y90 | Phosphorylation | Uniprot | |
K91 | Acetylation | Uniprot | |
K91 | Ubiquitination | Uniprot | |
K96 | Acetylation | Uniprot | |
K96 | Ubiquitination | Uniprot | |
T105 | Phosphorylation | Uniprot | |
T113 | Phosphorylation | Uniprot | |
T114 | Phosphorylation | Uniprot | |
T115 | Phosphorylation | Uniprot | |
K125 | Acetylation | Uniprot | |
K125 | Ubiquitination | Uniprot | |
K130 | Acetylation | Uniprot | |
K130 | Ubiquitination | Uniprot | |
S132 | Phosphorylation | Uniprot | |
K133 | Acetylation | Uniprot | |
K133 | Ubiquitination | Uniprot | |
R141 | Methylation | Uniprot | |
K156 | Acetylation | Uniprot | |
K157 | Acetylation | Uniprot | |
S159 | Phosphorylation | Uniprot | |
K160 | Ubiquitination | Uniprot | |
T164 | Phosphorylation | Uniprot | |
K180 | Acetylation | Uniprot | |
S187 | Phosphorylation | Uniprot | |
K191 | Acetylation | Uniprot | |
K191 | Ubiquitination | Uniprot | |
K192 | Acetylation | Uniprot | |
K196 | Ubiquitination | Uniprot | |
T200 | Phosphorylation | Uniprot | |
K202 | Acetylation | Uniprot | |
K202 | Ubiquitination | Uniprot | |
T206 | Phosphorylation | Uniprot | |
K218 | Acetylation | Uniprot | |
K218 | Ubiquitination | Uniprot | |
R221 | Methylation | Uniprot | |
Y223 | Phosphorylation | Uniprot | |
S225 | Phosphorylation | Uniprot | |
Y227 | Phosphorylation | Uniprot | |
T231 | Phosphorylation | Uniprot | |
S232 | Phosphorylation | Uniprot | |
K233 | Acetylation | Uniprot | |
K233 | Ubiquitination | Uniprot | |
K236 | Acetylation | Uniprot | |
K236 | Ubiquitination | Uniprot | |
C237 | S-Nitrosylation | Uniprot | |
Y243 | Phosphorylation | Uniprot | |
S247 | Phosphorylation | Uniprot | |
K249 | Acetylation | Uniprot | |
K249 | Ubiquitination | Uniprot | |
K250 | Ubiquitination | Uniprot | |
S252 | Phosphorylation | Uniprot | |
S253 | Phosphorylation | Uniprot | |
S256 | Phosphorylation | Uniprot | |
K269 | Acetylation | Uniprot | |
K292 | Methylation | Uniprot | |
K292 | Ubiquitination | Uniprot | |
K301 | Ubiquitination | Uniprot | |
K352 | Acetylation | Uniprot | |
K352 | Ubiquitination | Uniprot | |
K359 | Acetylation | Uniprot | |
K359 | Ubiquitination | Uniprot | |
K364 | Acetylation | Uniprot | |
K369 | Acetylation | Uniprot | |
K369 | Ubiquitination | Uniprot | |
T381 | Phosphorylation | Uniprot | |
T382 | Phosphorylation | Uniprot | |
S383 | Phosphorylation | Uniprot | |
Y385 | Phosphorylation | Uniprot | |
K387 | Acetylation | Uniprot | |
K389 | Acetylation | Uniprot | |
K396 | Acetylation | Uniprot | |
K396 | Ubiquitination | Uniprot | |
S398 | Phosphorylation | Uniprot | |
T409 | Phosphorylation | Uniprot | |
S410 | Phosphorylation | Uniprot | |
K417 | Acetylation | Uniprot | |
K417 | Ubiquitination | Uniprot | |
T422 | Phosphorylation | Uniprot | |
T428 | Phosphorylation | Uniprot | |
C442 | S-Nitrosylation | Uniprot | |
C447 | S-Nitrosylation | Uniprot | |
T455 | Phosphorylation | Uniprot | |
K462 | Acetylation | Uniprot | |
K469 | Acetylation | Uniprot | |
K469 | Ubiquitination | Uniprot | |
T471 | Phosphorylation | Uniprot | |
K473 | Acetylation | Uniprot | |
K473 | Ubiquitination | Uniprot | |
T478 | Phosphorylation | Uniprot | |
K481 | Ubiquitination | Uniprot | |
S488 | Phosphorylation | Uniprot | |
S498 | Phosphorylation | Uniprot | |
S499 | Phosphorylation | Uniprot | |
Y503 | Phosphorylation | Uniprot | |
K516 | Acetylation | Uniprot | |
K516 | Methylation | Uniprot | |
K516 | Ubiquitination | Uniprot | |
T522 | Phosphorylation | Uniprot | |
K523 | Acetylation | Uniprot | |
K523 | Ubiquitination | Uniprot | |
T527 | Phosphorylation | Uniprot | |
T540 | Phosphorylation | Uniprot | |
K551 | Acetylation | Uniprot |
研究背景
Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Mitochondrion matrix.
Homoheptamer arranged in a ring structure. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. Interacts with 2 heptameric Hsp10 rings to form the symmetrical football complex. Interacts with HRAS (By similarity). Interacts with ATAD3A. Interacts with ETFBKMT and METTL21B. Interacts with MFHAS1.
(Microbial infection) Interacts with hepatits B virus/HBV protein X.
(Microbial infection) Interacts with HTLV-1 protein p40tax.
Belongs to the chaperonin (HSP60) family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
文献引用
Application: WB Species: Mouse Sample: Hepa1-6 cells
Application: WB Species: human Sample:
Application: IF/ICC Species: human Sample:
限制条款
产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.