HSP60 Antibody - #AF0184

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产品: HSP60 抗体
货号: AF0184
描述: Rabbit polyclonal antibody to HSP60
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Xenopus
分子量: 68kDa; 61kD(Calculated).
蛋白号: P10809
RRID: AB_2833377

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(80%), Sheep(100%), Rabbit(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
HSP60 Antibody detects endogenous levels of total HSP60.
RRID:
AB_2833377
引用格式: Affinity Biosciences Cat# AF0184, RRID:AB_2833377.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; CH60_HUMAN; Chaperonin 60; Chaperonin, 60-KD; CPN60; fa04a05; GROEL; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 1 (chaperonin); Heat shock protein 60; Heat shock protein 65; heat shock protein family D (Hsp60) member 1; HLD4; Hsp 60; HSP 65; HSP-60; HSP60; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13;

抗原和靶标

免疫原:
Uniprot:

P10809(CH60_HUMAN) >>Visit HPA database.

基因/基因ID:
HSPD1(3329)
描述:
HSP60 Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Belongs to the chaperonin (HSP60) family. Interacts with HBV protein X and HTLV-1 protein p40tax.
序列:
MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGAMGGMGGGMGGGMF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Xenopus
100
Rabbit
100
Horse
80
Chicken
67
Pig
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P10809 作为底物

Site PTM Type Enzyme
R3 Methylation
K31 Acetylation
K31 Ubiquitination
K58 Ubiquitination
T61 Phosphorylation
S67 Phosphorylation
S70 Phosphorylation
K72 Ubiquitination
T74 Phosphorylation
K75 Ubiquitination
T79 Phosphorylation
K82 Acetylation
K82 Ubiquitination
S83 Phosphorylation
K87 Acetylation
K87 Ubiquitination
Y90 Phosphorylation
K91 Acetylation
K91 Ubiquitination
K96 Acetylation
K96 Ubiquitination
T105 Phosphorylation
T113 Phosphorylation
T114 Phosphorylation
T115 Phosphorylation
K125 Acetylation
K125 Ubiquitination
K130 Acetylation
K130 Ubiquitination
S132 Phosphorylation
K133 Acetylation
K133 Ubiquitination
R141 Methylation
K156 Acetylation
K157 Acetylation
S159 Phosphorylation
K160 Ubiquitination
T164 Phosphorylation
K180 Acetylation
S187 Phosphorylation
K191 Acetylation
K191 Ubiquitination
K192 Acetylation
K196 Ubiquitination
T200 Phosphorylation
K202 Acetylation
K202 Ubiquitination
T206 Phosphorylation
K218 Acetylation
K218 Ubiquitination
R221 Methylation
Y223 Phosphorylation
S225 Phosphorylation
Y227 Phosphorylation
T231 Phosphorylation
S232 Phosphorylation
K233 Acetylation
K233 Ubiquitination
K236 Acetylation
K236 Ubiquitination
C237 S-Nitrosylation
Y243 Phosphorylation
S247 Phosphorylation
K249 Acetylation
K249 Ubiquitination
K250 Ubiquitination
S252 Phosphorylation
S253 Phosphorylation
S256 Phosphorylation
K269 Acetylation
K292 Methylation
K292 Ubiquitination
K301 Ubiquitination
K352 Acetylation
K352 Ubiquitination
K359 Acetylation
K359 Ubiquitination
K364 Acetylation
K369 Acetylation
K369 Ubiquitination
T381 Phosphorylation
T382 Phosphorylation
S383 Phosphorylation
Y385 Phosphorylation
K387 Acetylation
K389 Acetylation
K396 Acetylation
K396 Ubiquitination
S398 Phosphorylation
T409 Phosphorylation
S410 Phosphorylation
K417 Acetylation
K417 Ubiquitination
T422 Phosphorylation
T428 Phosphorylation
C442 S-Nitrosylation
C447 S-Nitrosylation
T455 Phosphorylation
K462 Acetylation
K469 Acetylation
K469 Ubiquitination
T471 Phosphorylation
K473 Acetylation
K473 Ubiquitination
T478 Phosphorylation
K481 Ubiquitination
S488 Phosphorylation
S498 Phosphorylation
S499 Phosphorylation
Y503 Phosphorylation
K516 Acetylation
K516 Methylation
K516 Ubiquitination
T522 Phosphorylation
K523 Acetylation
K523 Ubiquitination
T527 Phosphorylation
T540 Phosphorylation
K551 Acetylation

研究背景

功能:

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homoheptamer arranged in a ring structure. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. Interacts with 2 heptameric Hsp10 rings to form the symmetrical football complex. Interacts with HRAS (By similarity). Interacts with ATAD3A. Interacts with ETFBKMT and METTL21B. Interacts with MFHAS1.

(Microbial infection) Interacts with hepatits B virus/HBV protein X.

(Microbial infection) Interacts with HTLV-1 protein p40tax.

蛋白家族:

Belongs to the chaperonin (HSP60) family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

文献引用

1). Morphine-induced microglial immunosuppression via activation of insufficient mitophagy regulated by NLRX1. Journal of Neuroinflammation, 2022 (PubMed: 35414088) [IF=9.3]
2). Lentinan induces apoptosis of mouse hepatocellular carcinoma cells through the EGR1/PTEN/AKT signaling axis. Oncology Reports, 2023 (PubMed: 37264970) [IF=4.2]

Application: WB    Species: Mouse    Sample: Hepa1-6 cells

Figure 2. Effects of LNT on the EGR1/PTEN/AKT axis in Hepa1-6 cells. (A) Protein expression levels of EGR1, PTEN, p-Akt and phosphorylation of Akt after treatment with a gradient of LNT concentrations as detected by WB. All data for protein expression were normalized using β-actin as a loading reference. (B) Immunofluorescence co-staining was used to assess the localization and expression of EGR1 and PTEN in Hepa1-6 cells treated with a gradient of LNT concentrations. Images were observed at ×630 magnification. Scale bars, 50 µm (white). (C) WB was used to detect the expression of EGR1 in the nuclear and cytoplasmic fractions following treatment with a gradient of LNT concentrations. (D) WB was used to detect the expression of EGR1, PTEN, p-Akt, and phosphorylation of Akt after EGR1 overexpression. *P
3). Hsp60 exerts a tumor suppressor function by inducing cell differentiation and inhibiting invasion in hepatocellular carcinoma. Oncotarget, 2016 (PubMed: 27677587)

Application: WB    Species: human    Sample:

A-B. qRT-PCR and western blot analyses for the mRNA and protein expression of Hsp60 in tumor and paired peritumor tissues from HCC patients.

Application: IF/ICC    Species: human    Sample:

E. Immunofluorescence analysis of the Hsp60 expression in BEL-7402 cell (Scale bars, 10μm)

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