| 产品: | GBA 抗体 |
| 货号: | DF8237 |
| 描述: | Rabbit polyclonal antibody to GBA |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Horse, Rabbit, Dog |
| 分子量: | 60 kDa; 60kD(Calculated). |
| 蛋白号: | P04062 |
| RRID: | AB_2841533 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8237, RRID:AB_2841533.
展开/折叠
Acid beta glucosidase; Acid beta-glucosidase; Alglucerase; Beta glucocerebrosidase; BETA GLUCOSIDASE, ACID; Beta-glucocerebrosidase; betaGC; D glucosyl N acylsphingosine glucohydrolase; D-glucosyl-N-acylsphingosine glucohydrolase; EC 3.2.1.45; GBA; Gba protein; GBA1; GC; GCase; GCB; GLCM_HUMAN; GLUC; Glucocerebrosidase (alt.); Glucocerebrosidase; GLUCOCEREBROSIDASE PSEUDOGENE; Glucosidase beta; Glucosidase, beta, acid; Glucosidase, beta; acid (includes glucosylceramidase); Glucosylceramidase; Imiglucerase; Lysosomal glucocerebrosidase; OTTHUMP00000033992; OTTHUMP00000033993;
抗原和靶标
- P04062 GLCM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P04062 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N58 | N-Glycosylation | Uniprot | |
| N98 | N-Glycosylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| N185 | N-Glycosylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| Y244 | Phosphorylation | Uniprot | |
| N309 | N-Glycosylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| Y412 | Phosphorylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| S523 | Phosphorylation | Uniprot |
研究背景
Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose. Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation. Also plays a role in cholesterol metabolism. May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol. The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction. Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide. Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol.
Lysosome membrane>Peripheral membrane protein>Lumenal side.
Note: Interaction with saposin-C promotes membrane association (PubMed:10781797). Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2 (PubMed:18022370).
Interacts with saposin-C. Interacts with SCARB2. Interacts with TCP1. May interact with SNCA; may inhibit the glucosylceramidase activity. Interacts with GRN; this interaction prevents aggregation of GBA-SCARB2 complex via interaction with HSPA1A upon stress.
Belongs to the glycosyl hydrolase 30 family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Metabolism > Glycan biosynthesis and metabolism > Other glycan degradation.
· Metabolism > Lipid metabolism > Sphingolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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