EEA1 Antibody - #DF8248

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产品: EEA1 抗体
货号: DF8248
描述: Rabbit polyclonal antibody to EEA1
应用: WB IHC IF/ICC
反应: Human
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 180 kDa; 162kD(Calculated).
蛋白号: Q15075
RRID: AB_2841542

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Bovine(83%), Horse(100%), Sheep(92%), Dog(92%)
克隆:
Polyclonal
特异性:
EEA1 Antibody detects endogenous levels of total EEA1.
RRID:
AB_2841542
引用格式: Affinity Biosciences Cat# DF8248, RRID:AB_2841542.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Early endosome antigen 1; Early endosome antigen 1, 162kD; Early endosome associated protein; EEA 1; EEA1; EEA1_HUMAN; Endosome associated protein p162; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; Zinc finger FYVE domain containing protein 2; Zinc finger FYVE domain-containing protein 2;

抗原和靶标

免疫原:
Uniprot:

Q15075(EEA1_HUMAN) >>Visit HPA database.

基因/基因ID:
EEA1(8411)
序列:
MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKEKVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTAKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDLQQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKKILKQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLKLELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQKTELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQDLKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQDIQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
92
Dog
92
Bovine
83
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15075 作为底物

Site PTM Type Enzyme
T9 Phosphorylation
S15 Phosphorylation
S18 Phosphorylation
S22 Phosphorylation
S23 Phosphorylation
T25 Phosphorylation
S49 Phosphorylation
S52 Phosphorylation
K58 Ubiquitination
S70 Phosphorylation
K81 Ubiquitination
S98 Phosphorylation
K100 Ubiquitination
S139 Phosphorylation
K158 Acetylation
K164 Acetylation
K164 Ubiquitination
K202 Ubiquitination
K228 Ubiquitination
T235 Phosphorylation
T241 Phosphorylation
S248 Phosphorylation
Y286 Phosphorylation
S295 Phosphorylation
S296 Phosphorylation
T301 Phosphorylation
S354 Phosphorylation
S356 Phosphorylation
S382 Phosphorylation
K422 Ubiquitination
K544 Ubiquitination
K552 Ubiquitination
T560 Phosphorylation
K592 Ubiquitination
S618 Phosphorylation
T621 Phosphorylation
S628 Phosphorylation
K634 Acetylation
S638 Phosphorylation
K645 Acetylation
T761 Phosphorylation
K824 Ubiquitination
T836 Phosphorylation
T837 Phosphorylation
K848 Methylation
T853 Phosphorylation
S856 Phosphorylation
T857 Phosphorylation
S863 Phosphorylation
K884 Ubiquitination
K895 Ubiquitination
T908 Phosphorylation
S926 Phosphorylation
K995 Ubiquitination
S1015 Phosphorylation
Y1021 Phosphorylation
S1024 Phosphorylation
T1027 Phosphorylation
K1029 Ubiquitination
K1125 Ubiquitination
K1133 Acetylation
K1138 Acetylation
K1142 Acetylation
T1159 Phosphorylation
K1178 Ubiquitination
K1183 Ubiquitination
T1252 Phosphorylation
S1265 Phosphorylation
K1312 Ubiquitination
K1319 Ubiquitination
K1340 Ubiquitination
T1392 Phosphorylation Q16539 (MAPK14)

研究背景

功能:

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

细胞定位:

Cytoplasm. Early endosome membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with RAB31. Interacts with ERBB2. Interacts with SAMD9 AND SAMD9L. May interact with PLEKHF2.

蛋白家族:

The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

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