PNP Antibody - #DF8260
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8260, RRID:AB_2841550.
展开/折叠
FLJ94043; FLJ97288; FLJ97312; Inosine phosphorylase; Inosine-guanosine phosphorylase; MGC117396; MGC125915; MGC125916; NP; Np1; Nucleoside phosphorylase; PNP; Pnp1; PNPH_HUMAN; PRO1837; PUNP; Purine nucleoside orthophosphate ribosyltransferase; Purine nucleoside phosphorylase 5a; Purine nucleoside phosphorylase;
抗原和靶标
Expressed in red blood cells; overexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.
- P00491 PNPH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MENGYTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASIPLPDKAS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P00491 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| K95 | Acetylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| R168 | Methylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K211 | Acetylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| T242 | Phosphorylation | Uniprot | |
| Y249 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| K254 | Acetylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| S289 | Phosphorylation | Uniprot |
研究背景
The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.
Cytoplasm>Cytoskeleton. Cytoplasm.
Expressed in red blood cells; overexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.
Homotrimer.
Belongs to the PNP/MTAP phosphorylase family.
研究领域
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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