PNP Antibody - #DF8260

Fig. 1. Elevation of inosine level promotes BC survival under glucose starvation. A MDA-MB-231 tumor tissues from the core and margin regions were separated to subject to RNA-seq and GSEA analysis, showing enrichment of genes related to the indicated pathway (n = 3 mice per group). B Representative mass spectrometry imaging for Inosine-5′-monophosphate abundance in MDA-MB-231 and 4T1 wild-type tumors from NSG mice and Balb/C mice. Scale bar, 2 mm. C Tissues from core and margin regions of 4T1 xenografted tumors were used for inosine detection. Inosine concentrations were measured by ELISA kit (paired two-tailed Student’s t-test, n = 7 mice per group). D Schematic diagram depicting inosine metabolic pathway. E The level of genes in purine pathway gene sets in the core and margin regions of MDA-MB-231 xenografted tumors are shown as a heat map. F Relative Nt5c2, Pnp, and Ada1 RNA levels in core and margin regions of 4T1 cells xenografted tumors determined by RT-qPCR (paired two-tailed Student’s t-test, n = 4 mice per group). G 4T1 tumor tissues were harvested from Balb/C mice. Tissues from the core and margin regions were separated to collect whole cell lysates, followed by western blots analysis. Indicated protein levels were assessed by western blots. H Representative IHC images showing indicated protein staining of core and margin regions tissues separated from 4T1 tumors. Scale bar, 50 μm. I Tumor volume followed in indicated xenografted mice (two-way ANOVA, n = 4 mice per group). J Representative tumor images of indicated xenografted tumors in Balb/C mice were shown (n = 8 mice per group). K Tumor weight was monitored at the end of the experiment in indicated xenografted mice (unpaired two-tailed Student’s t-test, n = 8 mice per group). L Tissues of 4T1 and 4T1/NT5C2 KD cells xenografted tumors were used for inosine detection. Inosine concentrations were measured by ELISA kit (paired two-tailed Student’s t-test, n = 6 mice per group). M Representative IHC images showing Ki67 staining of core regions tissues separated from 4T1 and 4T1/NT5C2 KD tumors. Scale bar, 50 μm. N MDA-MB-231 labeled Lck-GFP (231/Lck-GFP) cells were treated with glucose–glutamine-deficient medium (-G-Q) or supplemented with inosine or glucose. The fluorescent images were taken at the indicated time. Scale bar, 500 μm. O Quantification of the fold change of the cell numbers for (N) (one-way ANOVA, n = 3 biological replicates).

Fig. 1. Elevation of inosine level promotes BC survival under glucose starvation. A MDA-MB-231 tumor tissues from the core and margin regions were separated to subject to RNA-seq and GSEA analysis, showing enrichment of genes related to the indicated pathway (n = 3 mice per group). B Representative mass spectrometry imaging for Inosine-5′-monophosphate abundance in MDA-MB-231 and 4T1 wild-type tumors from NSG mice and Balb/C mice. Scale bar, 2 mm. C Tissues from core and margin regions of 4T1 xenografted tumors were used for inosine detection. Inosine concentrations were measured by ELISA kit (paired two-tailed Student’s t-test, n = 7 mice per group). D Schematic diagram depicting inosine metabolic pathway. E The level of genes in purine pathway gene sets in the core and margin regions of MDA-MB-231 xenografted tumors are shown as a heat map. F Relative Nt5c2, Pnp, and Ada1 RNA levels in core and margin regions of 4T1 cells xenografted tumors determined by RT-qPCR (paired two-tailed Student’s t-test, n = 4 mice per group). G 4T1 tumor tissues were harvested from Balb/C mice. Tissues from the core and margin regions were separated to collect whole cell lysates, followed by western blots analysis. Indicated protein levels were assessed by western blots. H Representative IHC images showing indicated protein staining of core and margin regions tissues separated from 4T1 tumors. Scale bar, 50 μm. I Tumor volume followed in indicated xenografted mice (two-way ANOVA, n = 4 mice per group). J Representative tumor images of indicated xenografted tumors in Balb/C mice were shown (n = 8 mice per group). K Tumor weight was monitored at the end of the experiment in indicated xenografted mice (unpaired two-tailed Student’s t-test, n = 8 mice per group). L Tissues of 4T1 and 4T1/NT5C2 KD cells xenografted tumors were used for inosine detection. Inosine concentrations were measured by ELISA kit (paired two-tailed Student’s t-test, n = 6 mice per group). M Representative IHC images showing Ki67 staining of core regions tissues separated from 4T1 and 4T1/NT5C2 KD tumors. Scale bar, 50 μm. N MDA-MB-231 labeled Lck-GFP (231/Lck-GFP) cells were treated with glucose–glutamine-deficient medium (-G-Q) or supplemented with inosine or glucose. The fluorescent images were taken at the indicated time. Scale bar, 500 μm. O Quantification of the fold change of the cell numbers for (N) (one-way ANOVA, n = 3 biological replicates).