| 产品: | UIMC1 抗体 |
| 货号: | DF8264 |
| 描述: | Rabbit polyclonal antibody to UIMC1 |
| 应用: | WB IHC |
| 反应: | Human |
| 预测: | Horse, Rabbit, Dog |
| 分子量: | 80 kDa; 80kD(Calculated). |
| 蛋白号: | Q96RL1 |
| RRID: | AB_2841553 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8264, RRID:AB_2841553.
展开/折叠
BRCA1-A complex subunit RAP80; Nuclear zinc finger protein RAP80; OTTHUMP00000161441; OTTHUMP00000223372; OTTHUMP00000223374; RAP80; Receptor associated protein 80; Receptor-associated protein 80; Retinoid X receptor interacting protein 110; Retinoid x receptor interacting protein; Retinoid X receptor-interacting protein 110; RIP110; Rxrip110; Ubiquitin interaction motif containing 1; Ubiquitin interaction motif containing protein 1; Ubiquitin interaction motif-containing protein 1; UIMC1; UIMC1_HUMAN; X2HRIP110;
抗原和靶标
Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
- Q96RL1 UIMC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPRRKKKVKEVSESRNLEKKDVETTSSVSVKRKRRLEDAFIVISDSDGEEPKEENGLQKTKTKQSNRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIAESLNSCRPSDASATRSRPLATGPSSQSHQEKTTDSGLTEGIWQLVPPSLFKGSHISQGNEAEEREEPWDHTEKTEEEPVSGSSGSWDQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQESTGRGSAFLKAVQGSGDTSRHCLPTLADAKGLQDTGGTVNYFWGIPFCPDGVDPNQYTKVILCQLEVYQKSLKMAQRQLLNKKGFGEPVLPRPPSLIQNECGQGEQASEKNECISEDMGDEDKEERQESRASDWHSKTKDFQESSIKSLKEKLLLEEEPTTSHGQSSQGIVEETSEEGNSVPASQSVAALTSKRSLVLMPESSAEEITVCPETQLSSSETFDLEREVSPGSRDILDGVRIIMADKEVGNKEDAEKEVAISTFSSSNQVSCPLCDQCFPPTKIERHAMYCNGLMEEDTVLTRRQKEAKTKSDSGTAAQTSLDIDKNEKCYLCKSLVPFREYQCHVDSCLQLAKADQGDGPEGSGRACSTVEGKWQQRLKNPKEKGHSEGRLLSFLEQSEHKTSDADIKSSETGAFRVPSPGMEEAGCSREMQSSFTRRDLNESPVKSFVSISEATDCLVDFKKQVTVQPGSRTRTKAGRGRRRKF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96RL1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Sumoylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K20 | Sumoylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| T24 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K31 | Sumoylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S46 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K59 | Ubiquitination | Uniprot | |
| K75 | Sumoylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K90 | Sumoylation | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| R97 | Methylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| K166 | Sumoylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| K215 | Ubiquitination | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| R239 | Methylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| K245 | Methylation | Uniprot | |
| K245 | Sumoylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K308 | Sumoylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| K382 | Sumoylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| T395 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S415 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| K480 | Sumoylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| K485 | Sumoylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| K544 | Sumoylation | Uniprot | |
| K544 | Ubiquitination | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| K559 | Sumoylation | Uniprot | |
| K559 | Ubiquitination | Uniprot | |
| K562 | Sumoylation | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K587 | Sumoylation | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| K607 | Sumoylation | Uniprot | |
| K607 | Ubiquitination | Uniprot | |
| K618 | Ubiquitination | Uniprot | |
| S627 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S632 | Phosphorylation | Uniprot | |
| K635 | Sumoylation | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| K642 | Ubiquitination | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| S677 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K680 | Acetylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| K696 | Acetylation | Uniprot | |
| K696 | Ubiquitination | Uniprot | |
| K697 | Ubiquitination | Uniprot | |
| T700 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | Uniprot |
研究背景
Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin Ref.37). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
Sumoylated.
Phosphorylated upon DNA damage by ATM or ATR.
Nucleus.
Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).
Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
Interacts with TSP57 (By similarity). Component of the ARISC complex, at least composed of UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with ABRAXAS1. Interacts with ESR1, NR6A1 and UBE2I.
The tandem UIM domains form a continuous 60 Angstrom-long alpha-helix and mediate binding to 'Lys-63'-linked ubiquitins. UIM1 and UIM2 bind to the proximal and distal ubiquitin moieties and recognize an 'Ile-44'-centered hydrophobic patch. Since UIMs don't interact with the 'Lys-63' isopeptide bond the UIM-linker region between the 2 UIM domains determines the selectivity for 'Lys-63'-linkage, and its length is very important for specificity.
The Abraxas-interacting region (AIR) mediates the interaction with ABRAXAS1.
Belongs to the RAP80 family.
研究领域
· Genetic Information Processing > Replication and repair > Homologous recombination.
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