Phospho-JAK1 (Tyr1022)[Tyr1034] Antibody - #AF2012

Fig. 3 The underlying mechanism by which aligned fibers affected macrophage polarization. A Venn diagram showing differentially expressed genes. B KEGG pathway analysis between the A20 and R20 groups. C Heatmap of differentially expressed genes among the three groups. D Heatmap of macrophage polarization-related genes between the A20 and R20 groups. E Volcano diagram of differentially expressed genes. F Western blot analysis of the NF-κB signaling pathway. G Immunofluorescence staining showing the nuclear translocation of NF-κB p65. The nucleus is stained blue, and NF-κB p65 protein is stained red. H Western blot images and semiquantitative analysis of the JAK-STAT signaling pathway (*p < 0.05, **p < 0.01, n = 3)

Fig. 6. Involvement of the JAK1/STAT3 pathway and the paracrine effects of irradiation-induced senescent BMSCs on osteoblasts osteogenic differentiation. A-B:Activation of JAK1/STAT3 pathway after irradiation of 10Gy, as shown in western blot analyses of p-JAK1, T-STAT3 and p-STAT3, while 0.8μM JAK1 inhibitor intervention could effectively inhibite JAK1/STAT3 pathway, and the downstream p-STAT3 expression was more significantly blocked. C: ELISA results show the upregulated secretion of IL-6, IL-8 and MMP9 in the supernatant collected 72h after Downloaded from journals.physiology.org/journal/ajpcell at Lunds Univ Medicinska Fak Biblio (130.235.066.010) on April 8, 2020.10Gy-irradiation and suppressed secretion after adding 0.8μM JAK1 inhibitor following irradiation. D-G: Representative images and quantitative analysis of ALP activity and mineralized nodule area of osteoblasts co-cultured with different types of CM from irradiated BMSCs (Con CM, Con/JAKi CM, IRIS CM and IRIS/JAKi CM).Magnification, ×100. H-I: Relative mRNA and protein expression levels of ALP and OC of osteoblasts co-cultured with four types of CM from irradiated BMSCs. All data were analyzed from three independent experiments. P values were calculated by Student’s t-test and one-way ANOVA analysis. Results are presented as mean ± SD.*p < 0.01, **p < 0.01, ***p < 0.001. ALP: alkaline phosphatase; OC: osteocalcin; Con CM: CM from control BMSCs; Con/JAKi CM: CM from control BMSCs with JAKi intervention; IRIS CM: CM from irradiation-induced senescent BMSCs; IRIS/JAKi CM: CM from irradiation-induced senescent BMSCs with JAKi intervention. SD:standard deviation. Scale bar: 100μm (D, F).

Figure 5. | GM-CSF increase the mRNA expression level of Nav1.7, Nav1.8, 822 Nav1.9 channel through Jak2-Stat3 signaling pathway. (A) Relative expression of 823 p-Jak1, p-Jak2, p-Jak3, p-stat3 and p-stat5 in DRG neurons after incubation with 824 GM-CSF for 25 mins. (n=3, unpaired t-test, *P < 0.05 as compared to control)

Figure 6. Effect of BCL2L10 on its downstream gene expression profiles of human cancer pathway in HepG2 cells. (A) By human cancer pathway PCR array, ectopic expression of BCL2L10 up- or down-regulated several genes related to tumor proliferation, apoptosis, metastasis and angiogenesis. (B) Western blot was performed to confirm the downstream gene expression regulated by BCL2L10 in HepG2 cells. GAPDH was used as an internal control. (C) Schematic diagram of the molecular events for BCL2L10 function as a tumor suppressor through regulating cell cycle, proliferation, apoptosis metastasis and angiogenesis effectors.

Figure 3.| The gemcitabine-erlotinib (E+G) combination group inhibits the activity of the JAK-STAT pathway, as well as the expression of downstream HIF‑1α, cyclin D1 and p53. (E) Phosphorylation levels of JAK1 (Tyr1022), JAK2 (Tyr221), JAK3(Tyr981) and STAT3 (Tyr701) in BxPC-3 and PANC‑1 cells were analyzed by western blotting, respectively.

Figure 5 A‐F, The expression of JAK1, p‐JAK1, JAK2, p‐JAK2, STAT1, and p‐STAT1 in macrophages within each group as determined by western blot analysis. G‐I, Quantitative analysis of the bands measured by gray value. The experiment was carried out a minimum of three times, and values are expressed as the mean ± SD. *P  < .05 and **P < .01 compared with MI. JAK, Janus kinase; STAT, signal transducer and activator of transcription [Color figure can be viewed at wileyonlinelibrary.com]

Figure 4: Relative mRNA and protein expression levels of JAK1, STAT6, and SOCS1.