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IF/ICC
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DF8316 staining Hela cells by IF/ICC.

Figure 2.

Figure 3.

产品:	PANX1 抗体
货号:	DF8316
描述:	Rabbit polyclonal antibody to PANX1
应用:	WB IF/ICC
反应:	Human, Mouse, Rat
分子量:	48 kDa; 48kD(Calculated).
蛋白号:	Q96RD7
RRID:	AB_2841594

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

克隆:

Polyclonal

特异性:

PANX1 Antibody detects endogenous levels of total PANX1.

RRID:

AB_2841594
引用格式: Affinity Biosciences Cat# DF8316, RRID:AB_2841594.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

innexin; MRS1; Pannexin 1; Pannexin-1; Panx1; PANX1_HUMAN; PX1; UNQ2529;

抗原和靶标

免疫原:

Uniprot:

Q96RD7(PANX1_HUMAN) >>Visit HPA database.

基因/基因ID:

PANX1(24145)

序列:

Q96RD7 PANX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAIAQLATEYVFSDFLLKEPTEPKFKGLRLELAVDKMVTCIAVGLPLLLISLAFAQEISIGTQISCFSPSSFSWRQAAFVDSYCWAAVQQKNSLQSESGNLPLWLHKFFPYILLLFAILLYLPPLFWRFAAAPHICSDLKFIMEELDKVYNRAIKAAKSARDLDMRDGACSVPGVTENLGQSLWEVSESHFKYPIVEQYLKTKKNSNNLIIKYISCRLLTLIIILLACIYLGYYFSLSSLSDEFVCSIKSGILRNDSTVPDQFQCKLIAVGIFQLLSVINLVVYVLLAPVVVYTLFVPFRQKTDVLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISEVKSYKCLKVLENIKSSGQGIDPMLLLTNLGMIKMDVVDGKTPMSAEMREEQGNQTAELQGMNIDSETKANNGEKNARQRLLDSSC

翻译修饰 - Q96RD7 作为底物

Q96RD7

Site	PTM Type	Source
Y10	Phosphorylation	Uniprot
Y150	Phosphorylation	Uniprot
S182	Phosphorylation	Uniprot
S189	Phosphorylation	Uniprot
Y199	Phosphorylation	Uniprot
K201	Ubiquitination	Uniprot
K203	Acetylation	Uniprot
K204	Acetylation	Uniprot
K212	Acetylation	Uniprot
N255	N-Glycosylation	Uniprot
K307	Ubiquitination	Uniprot
K343	Ubiquitination	Uniprot
K355	Ubiquitination	Uniprot
K381	Ubiquitination	Uniprot
S385	Phosphorylation	Uniprot
K409	Ubiquitination	Uniprot
S425	Phosphorylation	Uniprot

研究背景

Q96RD7_PANX1_HUMAN

功能:

Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.

翻译修饰:

S-nitrosylation inhibits channel currents and ATP release.

细胞定位:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction. Endoplasmic reticulum membrane>Multi-pass membrane protein.

亚基结构:

Homohexamer. Forms homomeric or PANX1/PANX2-heteromeric intercellular channels on coexpression in paired Xenopus oocytes (By similarity).

蛋白家族:

Belongs to the pannexin family.

研究领域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

文献引用

1). Processing of angiocrine alarmin IL-1α in endothelial cells promotes lung and liver fibrosis. International immunopharmacology, 2024 (PubMed: 38723369) [IF=5.6]

2). Carbenoxolone has the potential to ameliorate acute incision pain in rats. Molecular Medicine Reports, 2021 (PubMed: 34013377) [IF=3.4]

Application: WB Species: Rat Sample:

Figure 2. Px1 and Cx43 expression in the spinal cords of IP model rats. Expression of Px1 and Cx42 in rats was detected via western blotting at (A) 2, (B) 4, (C) 6 and (D) 24 h post-surgery. The scanning density of (E) Px1 and (F) Cx43 expression normalized to control. *P<0.05, **P<0.01 and ***P<0.001 vs. C group; #P<0.05, ##P<0.01 and ###P<0.001 vs. NS group; ΔP<0.05, ΔΔP<0.01 and ΔΔΔP<0.001 vs. CBX group. Data are presented as the mean ± SD. C, control group; IP, incision pain; NS, normal saline; CBX, carbenoxolone; 10panx, pannexin-1 mimetic inhibitory peptide; Px1, pannexin 1; Cx43, connexin 43.

Application: IF/ICC Species: Rat Sample:

Figure 3. Co-localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red) expression was analyzed using an immunofluorescence assay. Magnification, ×200; scale bar, 10 µm. C, control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin-1 mimetic inhibitory peptide; Px1, pannexin 1; GFAP, glial fibrillary acidic protein.

Application: IF/ICC Species: rat Sample:

Figure 3. |Co‑localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red)expression was analyzed using an immunofluorescence assay.Magnification, x200; scale bar, 10 µm. C,control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin‑1 mimetic inhibitory peptide; Px1, pannexin 1;GFAP, glial fibrillary acidic protein

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PANX1 Antibody - #DF8316

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