IL33 Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IHC
Cites

1/10

Western blot analysis of extracts from RAW264.

Western blot analysis of extracts from HepG2, using IL33 Antibody.

DF8319 at 1/100 staining Human liver tissue by IHC-P.

DF8319 at 1/100 staining Mouse ovary tissue by IHC-P.

FIGURE 6|The pro-tumour effect of IL-33 in vivo.

FIGURE 4|IL-33 promotes M2 macrophage polarization via ornithine decarboxylase (ODC) activation.

FIGURE 1|M2 macrophage infiltration and IL-33 production are enhanced with close correlation in oesophageal squamous cell carcinoma (ESCC).

Figure 1 IL33 is aberrantly expressed in the endometria of women with adenomyosis.

Fig.

产品:	IL33 抗体
货号:	DF8319
描述:	Rabbit polyclonal antibody to IL33
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
分子量:	31 kDa; 31kD(Calculated).
蛋白号:	O95760
RRID:	AB_2841596

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

克隆:

Polyclonal

特异性:

IL33 Antibody detects endogenous levels of total IL33.

RRID:

AB_2841596
引用格式: Affinity Biosciences Cat# DF8319, RRID:AB_2841596.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

C9orf26; CHROMOSOME 9 OPEN READING FRAME 26; DKFZp586H0523; DVS27; DVS27 related protein; IL 1F11; IL 33; IL-1F11; IL-33; IL1F11; IL33; IL33_HUMAN; Interleukin 1 family member 11; Interleukin 33; INTERLEUKIN 33 NFHEV; Interleukin 33 precursor; Interleukin-1 family member 11; Interleukin-33 (109-270); Interleukin33; NF HEV; NF-HEV; NFEHEV; NFHEV; Nuclear factor for high endothelial venules; Nuclear factor from high endothelial venules; OTTHUMP00000021041; RP11 575C20.2;

抗原和靶标

免疫原:

Uniprot:

O95760(IL33_HUMAN) >>Visit HPA database.

基因/基因ID:

IL33(90865)

表达:

O95760 IL33_HUMAN:

Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.

序列:

O95760 IL33_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKPKMKYSTNKISTAKWKNTASKALCFKLGKSQQKAKEVCPMYFMKLRSGLMIKKEACYFRRETTKRPSLKTGRKHKRHLVLAACQQQSTVECFAFGISGVQKYTRALHDSSITGISPITEYLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET

翻译修饰 - O95760 作为底物

O95760

Site	PTM Type	Enzyme	Source
K16	Acetylation		Uniprot

研究背景

O95760_IL33_HUMAN

功能:

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.

In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.

翻译修饰:

The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains. Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation. In vitro proteolytic cleavage by CASP1 was reported but could not be confirmed in vivo suggesting that IL33 is probably not a direct substrate for that caspase.

细胞定位:

Nucleus. Chromosome. Cytoplasmic vesicle>Secretory vesicle. Secreted.
Note: Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).

组织特异性:

Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.

亚基结构:

Forms a 1:1:1 heterotrimeric complex with its primary high-affinity receptor IL1RL1 and the coreceptor IL1RAP.

(Microbial infection) Interacts (in reduced form) with H.polygyrus ARI.

蛋白家族:

The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.

Belongs to the IL-1 family. Highly divergent.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)

文献引用

1). Honokiol suppresses the aberrant interactions between renal resident macrophages and tubular epithelial cells in lupus nephritis through the NLRP3/IL-33/ST2 axis. Cell Death & Disease, 2023 (PubMed: 36859530) [IF=9.0]

Application: WB Species: Mouse Sample:

Fig. 6: NLRP3 regulates IL-33 expression in renal resident macrophages.

2). Oesophageal squamous cell carcinoma–associated IL‐33 rewires macrophage polarization towards M2 via activating ornithine decarboxylase. CELL PROLIFERATION, 2021 (PubMed: 33305406) [IF=8.5]

Application: IHC Species: mouse Sample: tumour

FIGURE 6|The pro-tumour effect of IL-33 in vivo. A, Xenograft tumour growth curve. B, Tumour weight on day 21 after the first administration in each group. C, The body weight change in mice. Results are expressed as mean ± SEM (n = 5; **P < .01, ***P < .001). D, Kaplan-Meier survival curves of mice received different treatments, n = 10 mice per group. E, H&E staining of tumour tissues (scale bar = 200 μm). ***P < .001. Bars indicate mean and SEM of triplicate experiments and show a representative experiment of at least 3 independent experiments performed for each panel

Application: IF/ICC Species: human Sample: M2 macrophages

FIGURE 4|IL-33 promotes M2 macrophage polarization via ornithine decarboxylase (ODC) activation. A, IL-10, TGF-β and IL12p35 protein levels were determined by Western blot in IL-33–induced M2 macrophage with or without knocking down ODC. B, Immunofluorescence staining for IL-33–induced M2 macrophages with or without knocking down ODC. The red signal represents the staining of ODC, and the green signal represents the staining of ARG1 (scale bar = 20 μm).

Application: WB Species: human Sample: non-tumour and tumour

FIGURE 1|M2 macrophage infiltration and IL-33 production are enhanced with close correlation in oesophageal squamous cell carcinoma (ESCC). A, Representative images of IL-33+ cell, CD206+ cell and CD68+ cell in non-tumour and tumour tissue (scale bar = 50 μm).B, Western blot analysis of IL-33 and CD206 expression in non-tumour and tumour tissues, and GAPDH was used as a reference control.

3). SUMOylated IL-33 in the nucleus stabilizes the transcription factor IRF1 in hepatocellular carcinoma cells to promote immune escape. Science Signaling, 2023 (PubMed: 36917642) [IF=7.3]

4). Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo. Antioxidants, 2022 (PubMed: 36421424) [IF=7.0]

5). Decreased intracellular IL-33 impairs endometrial receptivity in women with adenomyosis. Frontiers in Endocrinology, 2022 (PubMed: 35937844) [IF=5.2]

Application: WB Species: Human Sample: endometria

Figure 1 IL33 is aberrantly expressed in the endometria of women with adenomyosis. (A) IL33 and HOXA10 protein expression in normal (n = 20) and patients with adenomyosis (n = 20) was analyzed by Western blot. (B) The intensities of IL33 signals were quantified from the 20 samples and normalized. to GAPDH. ***p < 0.001. (C) The intensities of HOXA10 signals were quantified from the 20 samples and. normalized to GAPDH. **p < 0.01. (D) Correlation between IL33 and HOXA10 protein expression levels (r = 0.7788, p < 0.0001). (E) Timed mid-secretory endometrial biopsies from healthy control and infertile women with adenomyosis were analyzed using immunohistochemistry (IHC). Rabbit or Goat IgG was used as the negative control. The arrows show the decreased IL33 and HOXA10 conjugates in the endometrial epithelium cell. (F) The integrated optical densities (IOD) of the expression of IL33 and HOXA10 in the endometrium using Image-Pro Plus System 6.0 image analysis software. (G) The mean density of the expression of IL33 and HOXA10 in the endometrium. using Image-Pro Plus System 6.0 image analysis software.

Application: IHC Species: Human Sample: endometria

6). Electroacupuncture prevents the development or establishment of chronic pain via IL-33/ST2 signaling in hyperalgesic priming model rats. Neuroscience letters, 2024 (PubMed: 38142925) [IF=2.5]

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IL33 Antibody - #DF8319

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